Relationship between progesterone level on the day of human chorionic gonadotropin administration with outcomes of intra-cytoplasmic sperm injection in infertile couples

Gonadotropin-releasmg hormone agonists or antagonists are used in assisted reproductive technique cycles as premature luteinizing hormone secretion inhibition. Studies have been reported different and contradictory results on the serum progesterone effect on intra-cytoplasmic sperm injection. The purposeof this study was to evaluate the effect of serum progesterone level on the day of Human chorionic gonadotropin [HCG] administration on the intra-cytoplasmic sperm injection [ICSI] outcome in infertile women. 249 infertile couples candidated for ICSI were enrolled in the study. Their serum progesterone level on the day of HCG administration was measured and according to serum level, patients were divided into four groups of less than 0.9, 0.9-1.4, 1.5-1.9, and 7/= ng/mL. The four groups were compared with each other regarding fertility outcomes. Pregnancy rate was not significantly different among the four groups [p>0,05]. Also, there was no significant difference among the groups regarding frequency of abortion and ectopic pregnancy. Serum progesterone level on the day of HCG administration does not have any significant effect on pregnancy outcomes, including abortion, ectopicpregnancy, and pregnancy rate in patients undergoing ICSI treatment

[Evaluation of serum estradiol/ progestrone ratio on day of embryo transfer and it's effect on intra- cytoplasmic sperm induction outcome]

One of the important problems in fertilization in vitro [IVF] is failure of implantation. This could be the result of estrogen and progesterone effects in endometrial acceptance during ovulation stimulation. Although progesterone has a vital role in primary phase of pregnancy, but the estradiol role in luteal phase is unknown. The aim of this study is assessment of the ratio of estradiol to progesterone in embryo transfer day on Intracytoplasmic sperm injection [ICSI] outcomes. This is a cohort study. The subjects were 311 infertile women referred to Urmia Kosar infertility clinic and Urmia reproductive health research center who treated with ICSI method between August-Jan 2011. Five cc blood was drawn for determine of estradiol and progesterone in transfer day. Transfer occurred after a variable in vitro culture period ranging from 48 to 72 hours after ovulation induction. Chemiluminescent ELICA the level of mention hormones was used to determine the ratio of the progesterone level to the estradiol serum level and was compared based on treatment outcomes. A total of 311 patients, 115 [37%] were pregnant and happened abortion were 18 [5.8%]. The mean ratio of estradiol to progesterone in transfer day in two groups of miscarriage/ non miscarriage and pregnant/ non pregnant was 32.26 +/- 23.86, 28.17 +/- 26.5 and28.58 +/- 2.4, 36.09 +/- 4.39 respectively. There is no significant difference between two groups in regard of estradiol on progesterone ratio [P=0.5, P=0.2]. The results of this prospective cohort study show that there is no effect of estradiol to progesterone ratio on day of embryo transfer, successful pregnancy in ICSI cycles and abortion rate following of ICSI.