ABSTRACT
Prostate cancer [PCa] is an important health problem in the aging male population in the world. It is the third most common cancer in the world. Despite of its importance, relatively little is known about its etiology. Sexually transmitted infections [STI] and urogenital pathogens such as Mycoplasma and Ureaplasma, have been proposed as a risk factor for prostate cancer development. This study aimed at detecting the prevalence of Ureaplasma urealyticum [U. urealyticum] and Mycoplasma genitalium [M. genitalium] in PCa and the controls group with benign prostate hyperplasia [BPH] in Shohada hospital. A total of 124 paraffin-embedded prostate tissues [62 PCa patients and 62 controls with BPH] were included in this study. The subjects'specimens were investigated by the polymerase chain reaction method for the presence of U. urealyticum and M. genitalium DNA. U. urealyticum was detected by standard PCR in 1.61% of the 62 PCa patients and there was no DNA U. urealyticum in the 62 controls with BPH. No M. genitalium was detected by standard PCR in the prostates of 124 paraffin-embedded prostate tissues. According to our results, there is no association between M. genitalium and U.urealyticum with PCa. We recommend further studies using a large sample to determine role of Ureaplasma and Mycoplasma in PCa because understanding the role of infectious agents on PCa might be useful for developing new therapeutic approaches and prevention of PCa
ABSTRACT
Assessment of the extent of the role of L. monocytogenes in human spontaneous abortions, using isolation methods and PCR analysis for the presence of actA and InlB genes. In this descriptive study, vaginal swabs were collected from 96 women with spontaneous abortions who referred to Tehran's hospitals. The samples were cultured in to Listeria specific media [PALCAM agar]. Then, the Listeria genus was verified by differentiation biochemical tests, such as, hemolysis on Blood agar, Catalase and Oxidase reactions, and motility at room temperature. PCR technique was performed on all samples and detected the actA and InlB genes of L. monocytogenes. In culture, 7 of 96 samples were positive for L.monocytogenes. With the PCR technique, actA and InlB genes were detected from 12 and 2 vaginal samples, respectively. The occurrence of pathogenic L. monocytogenes in cases of spontaneous abortions was 12.5%. It seems that the actA and InlB genes have important roles as essential virulence factors in pathogenic L.monocytogenes. The results show the PCR method is more sensitive, easier and faster than culture to detect L.monocytogenes