ABSTRACT
A new series of A-[5-Mercapto-1.3.4-thiadiazol-2-yl]-2-phenylacetamide derivatives [3a-3j] were synthesized via an amidation reaction using EDC and HOBt in acetonitrile solvent at room temperature condition. Chemical structures were characterized by [1]H NMR, IR and MS spectroscopic methods and related melting points were also determined. The anticancer activity was evaluated using MTT procedure in-vitro. All compounds were tested against SKNMC [Neuroblastoma], HT-29 [Colon cancer] and PC3 [Prostate cancer] cell lines. According to the toxicological data, none of the synthesized derivatives exerted superior activity than doxorubicin as reference drug. Derivatives with Ortho chlorine [compound 3d], meta methoxy [compound 3h] and meta fluorine [compound 3b] substituents on the phenyl ring exhibited the best cytotoxic activity against SKNMC [IC[50] = 4.5 +/- 0.035 microM], HT-29 [IC[50] = 3.1 +/- 0.030 microM] and PC3 [IC[50] - 12.6 +/- 0.302 microM] cell lines respectively