Percepção de oportunidades de aprendizado de funcionários técnico-administrativos no âmbito universitário / Perception learning opportunities of technical-administrative staff in the university scope / Percepción del personal técnico y administrativo sobre las oportunidades de aprendizaje en las universidades

Resumo Este estudo tem por objetivo identificar a percepção de oportunidades de aprendizagem e sua relação com o estilo de liderança exercido pelos funcionários técnico-administrativos no âmbito universitário, a intenção de rotatividade e capital psicológico no trabalho. Foi realizado um estudo transversal, com 102 funcionários técnico-administrativos, por meio de um questionário de autopreenchimento contendo dados referentes a idade, escolaridade, tempo de serviço na instituição e se o respondente exercia um cargo de liderança. Foram aplicadas escalas relacionadas à percepção de oportunidades de aprendizagem, avaliação do estilo gerencial, intenção de rotatividade e capital psicológico no trabalho. Os resultados revelam que os funcionários identificam oportunidades de aprendizado e a presença de três estilos gerenciais no âmbito universitário. Também se observou que os funcionários se identificam como capitais psicológicos no trabalho e possuem baixa intenção de rotatividade. Assim, este trabalho buscou ampliar a literatura ainda escassa envolvendo funcionários técnico-administrativos em âmbito universitário. (AU)

Abstract This study aimed to identify the perception of learning opportunities and its association with the leadership style of the administrative-technical staff within the university scope, the turnover intention, and the psychological capital at work. For that, this cross-sectional study was conducted with data on age, education level, length of service in the institution, and leadership position of 102 administrative and technical employees, collected by means of a self-administered questionnaire. Perceived learning opportunities, management style, turnover intention, and psychological capital at work were evaluated using scales. The results show that the administrative-technical staff identifies the three management styles and learning opportunities within the university scope. Moreover, they identify themselves as psychological capitals at work and possess low turnover intention. This study is expected to expand the (still scarce) literature on administrative and technical staff in the university context. (AU)

Resumen Este estudio tuvo como objetivo identificar las oportunidades de aprendizaje y su relación con el estilo de liderazgo adoptado por el personal técnico y administrativo en las universidades, la intención de rotación y el capital psicológico en el trabajo. Este es un estudio transversal realizado con 102 personales técnicos y administrativos mediante la aplicación de un cuestionario de autoinforme con datos sobre la edad, la educación, el tiempo de servicio en la institución y si ejercían cargo de liderazgo. Se aplicaron las escalas en cuanto a la percepción de las oportunidades de aprendizaje, la evaluación del estilo de gestión, la intención de rotación y el capital psicológico en el trabajo. Los resultados muestran que el personal identifica oportunidades de aprendizaje y la presencia de tres estilos de gestión en la universidad. También se observó que ellos se identifican como capital psicológico en el trabajo y tienen la intención de baja rotación. Por lo tanto, este estudio trató de ampliar la literatura todavía escasa, con la participación del personal técnico y administrativo en el ámbito universitario. (AU)

How has dental pulp stem cells isolation been conducted? A scoping review

Abstract: The objective of this study was to realize a scoping review the literature in order to identify the profile of DPSCs isolation and analyze the possible risk factors that could change the native behavior of these cells. An initial search was conducted using the following MeSH terms: "(dental pulp stem cell [MeSH])"; "(dental pulp [MeSH])" AND "(stem cell [MeSH])"; "("dental pulp stem cell" [MeSH]")". The electronic search was done without date restriction up to and including April 2014, in PubMed, Scopus, Scielo and ISI Web of Knowledge databases. Studies were submitted to inclusion and exclusion criteria and 222 articles were included. Data showed that over the past 15 years many studies have been conducted using DPSCs. However this is the first systematic review regarding the isolation of stem cell, and more specifically of dental pulp stem cells. The isolation of dental pulp stem cells showed great variability, hampering the development of standard protocols to achieve in vitro dental pulp stem cells with similar characteristics. This scoping review combined, for the first time, the methodologies used for dental pulp stem isolation, highlighting the most frequently used.

Etiologic factors associated with oral squamous cell carcinoma in non-smokers and non-alcoholic drinkers: a brief approach

Oral squamous cell carcinoma (OSCC) is the fifth most common type of cancer in the world. The effect of some etiological factors is well established in the literature, such as consumption of tobacco and alcohol. However, approximately 15 to 20% of all oral cancer cases occur in patients without the traditional risk factors, reflecting in numerous cases of OSCC in non-smokers and non-alcoholic drinkers. Also, several studies have suggested a possible association between human papillomavirus and OSCC. Under these aspects, the purpose of this study is to address cases of oral cancer in non-smokers and non-drinkers focusing on the role of HPV, thus contributing to improve the diagnosis and monitoring of OSCC. A computer database search was performed using the Pubmed database. The search key words were: epidemiology, oral squamous cell carcinoma, risk factors and human papillomavirus. It is important to assess patients without the potential risk factors, since this knowledge may help identifying other features associated with the occurrence of oral cancer, enabling an appropriate clinical management and monitoring.

O carcinoma espinocelular (CEC) de boca é o quinto mais comum no mundo. O efeito de alguns fatores etiológicos está bem estabelecido na literatura, como o fumo e o consumo de álcool. No entanto, aproximadamente 15 a 20% dos casos de câncer bucal acometem pacientes sem os tradicionais fatores de risco e isso se traduz em inúmeros casos de CEC bucais em indivíduos não fumantes e não etilistas. Além disso, dados da literatura têm sugerido uma possível associação entre o vírus papiloma humano (HPV) e o carcinoma espinocelular de boca. Em virtude desse aspecto, desenvolveu-se este trabalho na perspectiva de revisar as características mais associadas a esse grupo de pacientes, contribuindo assim para a melhoria no diagnóstico e no acompanhamento dos mesmos. Os artigos que serviram de apoio para o estudo foram localizados por meio da base de dados Pubmed, utilizando os descritores "epidemiologia", "carcinoma espinocelular de boca", "fatores de risco" e "vírus papiloma humano". É importante avaliar pacientes sem tais fatores de risco, uma vez que o conhecimento pode auxiliar na análise de outras características associadas com a ocorrência de carcinoma. Além disso, possibilita melhorar o manejo clínico e monitoramento.

Buccal cells submitted to three different storage conditions before DNA extraction

This study evaluated quantitatively and qualitatively the effect of the storage time of samples before the application of the cell lysis solution (CLS) for extracting DNA from buccal cells (BC). BC from the upper and lower gutter region were collected from 5 volunteers using special cytobrushes (Gentra), totaling 3 collections for each individual. In the control group (n=10), CLS was applied soon after BC collection. In the other two groups, samples were stored at room temperature (n=10) or at 4°C (n=10). After CLS application, DNA was extracted according to the manufacturer's instructions (Puregene DNA Buccal Cell Kit; Gentra Systems, Inc.). The DNA obtained was evaluated by two calibrated blind examiners using spectrophotometry and analysis of DNA bands (0.8 percent agarose gel electrophoresis). The obtained data were submitted to one-way ANOVA. The means and standard deviations for DNA extracted under immediate, room temperature and cooling temperature conditions were 3.5 ± 0.7, 3.0 ± 0.6 and 4.1 ± 1.8 µg, respectively (p=0.385). No significant differences were found in relation to the amount of DNA for the different storage conditions. However, in the visual analysis of the DNA bands, no trace of DNA degradation was detected when CSL was applied soon after DNA collection, while DNA bands with degradation could be observed in the other groups. Within the limitations of the study, it may be concluded that CLS should be applied soon after DNA collection in order to obtain high-quality DNA from BC.

Evaluation of identification cases involving forensic dentistry in the city of Pelotas, RS, Brazil, 2004-2006

Aim: This study investigated the use of dental arch examination as a forensic technique for body identification at the Institute of Forensic Medicine (IML, acronym in Portuguese) of Pelotas (RS), a city located in the South of Brazil. Methods: The data collected for the study referred to the period between 2004 and 2006, when a forensic dentist was part of the IML staff. The post-mortem records with regard to the entry of unidentified bodies that had undergone dental identification by the forensic dentists were analyzed quantitatively. Results: Ten unidentified bodies entered the IML and all of them were submitted to dental arch examination for body identification. However, the conclusive identification was based on the analysis of DNA, because the victims’ dental records were not accurate, complete and updated. Only five assessed bodies had been referred from Pelotas police station, two from Pedro Osório police station, one from Capão do Leão police station, one from Arroio Grande police station and one had no information about its origin. Conclusions: The current configuration of Pelotas IML staff does not include a forensic dentist, and the presence of this professional is needed, for the city is reference for referral of forensic cases from the surrounding region.

Comparison between DNA obtained from buccal cells of the upper and lower gutter area

This study compared quantitatively and qualitatively the DNA extracted from buccal cells collected from the upper or lower gutter areas. Buccal cells were collected from the upper (n=15) and lower gutter (n=15) region from 15 volunteers using a special cytobrush (Gentra), totaling 2 collections from each individual. DNA was extracted from the samples according to the manufacturer's instructions. The DNA obtained was qualitatively and quantitatively evaluated by 2 calibrated blind examiners using spectrophotometry and analysis of DNA bands (0.8 percent agarose gel electrophoresis). Data was statistically analyzed by one-way ANOVA (?=0.05). Means and standard derivation (SD) for total DNA yield from the upper and lower gutter area were 12.2 ?g (12.0) and 9.4 ?g (8.5), respectively (p=0.821). There was higher (p<0.05) DNA purity for the upper gutter (1.79; 0.05) when compared to lower gutter area (1.66; 0.10). Regarding to the DNA quality, no differences were observed between the 2 location sites, but all samples showed similar degree of degradation. In conclusion, it would be recommendable that buccal cells for DNA extraction be collected from the upper gutter area in the attempt to increase DNA purity.

O objetivo do presente estudo foi comparar quantitativamente e qualitativamente o DNA extraído de células epiteliais bucais coletadas do fundo de sulco superior e inferior. Foram coletadas células bucais do fundo de sulco superior (n=15) e inferior (n=15) de 15 voluntários utilizando escovas citológicas especiais (Gentra), totalizando 2 coletas por voluntário. Após a coleta o DNA foi extraído conforme o protocolo indicado pelo fabricante (Puregene DNA Buccal Cell Kit; Gentra Systems, Inc.). O DNA obtido foi avaliado quantitativamente e qualitativamente por dois examinadores calibrados cegos utilizando espectrofotometria e análise das bandas de DNA (gel de agarose 0,8 por cento, por eletroforese). Os dados foram submetidos a ANOVA a um critério, com p<0,05. As médias e desvio padrão (DP) para o rendimento total de DNA do fundo de sulco superior e inferior foram respectivamente 12,2 ?g (12,0) e 9,4 ?g (8,5) (p=0,821). Houve maior (p<0,05) pureza de DNA no fundo de sulco superior (1,79; 0,05) quando comparado com o fundo de sulco inferior (1,66; 0,10). Quanto à qualidade do DNA, não foi observado diferenças entre os dois locais testados, no entanto todas as amostras mostraram níveis de degradação semelhantes. Em conclusão seria recomendável coletar células bucais, para extração de DNA, do fundo de sulco superior na tentativa de aumentar a pureza do DNA.