ABSTRACT
Today, the consumers have more knowledge about the unwanted effects of synthetic antimicrobials in foods and they want food with more natural ingredients. There are many researches about antimicrobial effects of essential oils, but we did not found researches about Zatariamultiflora Bios on E.coli O157: H7 in minced beef. The aim of this study was to determinre the effect of Zatariamultiflora Bios essential oil on E.coli O157: H7 in minced. The antimicrobial effect of different concentrations of Zatariamultiflora Boiss essential oil at supplementation levels of [0, 0.005, 0.015, 0.03%] on E. coli O157: H7 was examined in minced beef. All of the above concentrations showed acceptable organoleptic properties in minced beef. Eo at 0.03% possessed a strong antibacterial activity against E.coli O157: H7 in minced beef. The correlation coefficient of different concentrations of Zatariamultiflora Boiss essential oil with logarithm of the numbers of E. coli O157: H7 was -0.701, -0.599 at 4 and 10°C respectively. It was found that effect of different concentrations of essential oil on growth rate of E. coli O157: H7 was statistically significant [p<0.01]. In this study, it was found that treatment of minced beef with different concentrations of essential oils showed an inhibitory activity against E. coli O157: H7 during storage at 4°C, but not at 10°C [p<0.01]. According to present study, storage at a proper refrigerated storage like 4°C is suggested. Zatariamultiflora Bioss essential oil can use as a natural preservative instead of chemical preservatives for meat products
ABSTRACT
Available drugs are not able to eradicate intracellular viral DNA in patients with hepatitis B virus [HBV] infections. HBsAg vaccine could induce immunity and subsequently eradicate Hepatitis B virus in proportions of these patients. Our aim was to evaluate the efficacy of HBsAg as a mode of therapy in inactive carriers. Forty two consecutive patients of inactive carriers were enrolled. All patients underwent liver biopsies. The modified Ishak score of all cases were less than 4. Twenty microgram of recombinant HBsAg vaccine injected intradermally 3 times [at 0, one and 6 months]. Biochemical and serological variables evaluated initially and 6 months after the last injection. The mean age was 39.6 +/- 11.12. Male/female ratio was [67.4%]. Two out of 42 cases lost their HBsAg [4.74%]. The difference was significant comparing to one percent annual spontaneous HBsAg loss [p=0.014]. In addition serum albumin level was significantly increased after vaccination [p=0.009]. Rest of the biochemical and serological variables had no significant changes comparing pre and post vaccination. Intradermal injection of HBV surface antigen vaccine could induce significant HBsAg loss. This mode of therapy is cheap, physiologic and without complication. However, the results of this study should be confirmed in further large controlled trial