ABSTRACT
The aim of this study is to compare Candida albicans strain genotype isolates from oral cavity of immunocompromised patients due to different immunologic impairments with apparently normal carriers. Four populations were studied: 1) HIV positive hospitalized patients, 2) HIV negative immunocompromised patients (leukemia, lymphoma, organ transplant recipients), 3) drug addicts prior to AIDS pandemia in Argentina, 4) apparently normal carriers. DNA extracted was digested with the enzyme Eco RI, electrophoresed, transferred to nitrocellulose membrane and hybridized with the 27A probe labelled with 32P. The comparison between the profiles obtained permitted the differentiation of 16 genotypes. The distribution of the strains led to the conclusion that: a) all the isolated strains from AIDS patients were closely related and distributed in only three genotypes (1, 3, 11); b) a major genetic relationship between the isolates from AIDS patients and HIV negative immunocompromised patients was observed; c) strains from carriers showed a minor genetic similarity with those obtained from AIDS patients; d) characteristic profiles belonging to any of the studied groups were not found; e) significant genomic changes have not been observed during the last twenty years.
ABSTRACT
Dentin treatment before adhesion of composites is performed both to enhance adhesion and to remove the microbial contents of the smear layer. The purpose of these experiments was to evaluate the germicide potential of several dentin treatments used in adhesive systems and of some cleansing solutions. Different germs involved in caries processes were used (Candida Albicans, Streptococcus mutans and Actinomyces naeslundii) to prepare suspensions. Half a milliliter of each of the suspensions was transferred to test tubes and an equal volume of the following substances was added: Scotch Prep Dentin Primer (P), Gluma Cleanser (G), Cleaner Sol. (C), Tubulicid Blue (TB) and Red Label (TR), Blue Experimental Solution (SB) and Red Experimental Solution (SR) and sterile distilled water (control). The preparation was incubated at 37 degrees C for seven days to test viability. P, TR, TB and SB produced complete inhibition of germs tested. The results reveal that, [quot ]in vitro[quot ], not all the substances tested exert a germicide effect on the microorganisms analyzed.
ABSTRACT
Se evaluo la inmunidad celular en 52 enfermos dragadictos, comparandola con un grupo de 30 no adictos. Para tal fin se utilizaron pruebas cutaneas con derivado proteinico purificado, candidina, parche de dinitroclorobenceno, prueba de la inhibibion de la migracion de linfocitos con derivado proteinico purificado de tuberculina y candidina, y ventana cutanea. Los resultados fueron diferentes en ambos grupos e indicarian que los toxicomanos tenian deprimida la respuesta celular