Potential Mechanism of Taraxaci Herba Against Bladder Cancer： A Review / 中国实验方剂学杂志

Bladder cancer （BCa） is the most common malignant tumor of the urinary system， and its incidence is increasing year by year. At present， for all patients with resectable non-metastatic muscle-invasive BCa， radical cystectomy + bilateral pelvic lymph node dissection is strongly recommended， but they still face the risk of recurrence， metastasis and death. In recent years， the proportion of patients with advanced and metastatic BCa is increasing among patients with newly diagnosed BCa. Although current treatment models are diverse， they often struggle to achieve significant efficacy due to their low effectiveness and adverse effects， resulting in low survival rates for patients with advanced and metastatic BCa. Therefore， the treatment of BCa still faces great challenges， and there is an urgent need to discover an effective new antitumor drug. With the improvement of medical standards， traditional Chinese medicine has shown great advantages in the treatment of BCa. Traditional Chinese medicine is mild and easy to accept， and can inhibit tumor progression through a multi-pathway， multi-way and multi-target manner， so as to exert its anticancer effect. Taraxaci Herba is a medicinal and food homologous plant， which has many biological activities， such as antibacterial， anti-inflammatory， anti-oxidation， anti-tumor， protecting liver and gallbladder， reducing blood sugar and enhancing immunity， and it has shown a clear anticancer effect in breast cancer， liver cancer， gastric cancer， tongue cancer and lung cancer. By reviewing previous studies worldwide， this article summarizes the mechanism of Taraxaci Herba extract in inducing autophagy and apoptosis， inhibiting cell migration and invasion， regulating cell cycle and proliferation， regulating cell metabolism， inhibiting tumor angiogenesis， combining the effects of chemotherapeutic drugs， and regulating the transduction of related signal pathways. On this basis， this study systematically elaborates on the potential mechanism of Taraxaci Herba against BCa， in order to provide a theoretical basis for the research and treatment of BCa.

Solitary renal duplication with misaligned malrotation of upper and lower moieties complicated by complete staghorn stones in the lower moiety: a case report / 中华泌尿外科杂志

Solitary kidney, renal duplication and malrotation are rare congenital renal malformations in urology department, and probably contributed to some complications such as obstruction, hydronephrosis, infection, stones. In this case report, we firstly presented a male patient with rarely multiple renal malformations, including solitary kidney, renal duplication, misaligned malrotation of upper and lower moieties, and accompanied by complete staghorn stones and hydronephrosis, who was treated with open pyelolithotomy under general anesthesia. After the operation, obstruction, hydronephrosis, and infection were relieved.

The Early Diagnostic Value of Dynamic Changes for High-sensitive Troponin I in Patients With Acute Coronary Syndrome / 中国循环杂志

Objective: To explore the early diagnostic value of dynamic changes for high-sensitive troponin I (hs-cTnI) in patients with acute coronary syndrome (ACS). Methods: A total of 186 ACS patients treated in our hospital from 2014-02 to 2015-04 were studied. The patients were divided into 2 groups:AMI group, n=169 and UA group, n=17, in addition, there was a Control group, n=13 healthy subjects. Blood levels of hs-cTnI were measured at admission and 2h, 4h after admission. Dynamic changes of hs-cTnI was calculated by slop coefifcient of hs-cTnI (Δhs-cTnI) at 3 time points and relative changes of hs-cTnI values. ROC curve was made by SPSS 16.0 statistical software and the best diagnostic cutoff point was determined by Youden index. Results: In clinical practice, hs-cTnI=1 ng/ml was critical condition, hs-cTnI=0.15 ng/ml was recommended for AMI diagnosis. When hs-cTnI Conclusion: AMI could not be effectively diagnosed by hs-cTnI alone at admission, using combined relative changes of hs-cTnI andΔhs-cTnI was superior to 0 h hs-cTnI for AMI diagnosis.

Thymidylate synthase genetic polymorphisms and cancer risk: a meta-analysis of 37 case-control studies / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Several studies have evaluated the association between polymorphisms of thymidylate synthase (TS) and cancer risk in diverse populations but with conflicting results. By pooling the relatively small samples in each study, it is possible to evaluate the association using a meta-analysis.</p><p><b>METHODS</b>A comprehensive search was conducted to identify all case-control studies on TS on a 28-bp tandem repeats in 5'untranslated region (5'UTR) and a 6-bp insertion (ins) and deletion (del) mutation in 3'UTR of the gene and cancer risk. Meta-analysis was conducted using a fixed and random effect model.</p><p><b>RESULTS</b>Our meta-analysis on a total of 13 307 cancer cases and 18 226 control subjects from 37 published case-control studies showed no significant association between the risk of cancer and the 5'UTR 28-bp tandem repeats polymorphism (3R/3R vs. 2R/2R: OR = 1.06, 95%CI, 0.93 - 1.20) or the 3'UTR 6-bp ins/del polymorphism (del6/del6 vs. ins6/ins6: OR = 0.93, 95%CI, 0.81 - 1.08) with significant between-study heterogeneity. In the cancer type- and ethnic subgroup-stratification analyses, we did not find any association between TS polymorphisms and cancer risk either.</p><p><b>CONCLUSION</b>TS 5'UTR 28-bp tandem repeats and 3'UTR 6-bp ins/del polymorphisms may not be associated with cancer risk.</p>

Role of HO/CO in IL-beta induced pancreatic islets apoptosis and the effect of fructose-1, 6-disphosphate / 中国应用生理学杂志

<p><b>AIM</b>To investigate the protective role of HO/CO systems in IL-1beta induced islest apoptosis and to explore the mechanisms of the protective effect of fructose-1, 6-disphosphate (FDP).</p><p><b>METHODS</b>The pancreases of the rats were removed to collect islets cells. The cells were incubated with IL-1beta with/or FDP. Cell activity, insulin secretion, HO-1 activity, CO content and apoptotic percentage were detected.</p><p><b>RESULTS</b>HO-1 activity and CO content of the normal control group were low. IL-1beta induced a significant decrease of cell activity and insulin release, flow cytometry analysis showed that apoptotic percentage of islet cells remarkably increased following the addition of IL-1beta, FDP obviously improved the islets cellular activity damaged by IL-1beta, and basic amount of insulin secretion and stimulated by high glucose were improved (P < 0.01). Content of CO and activity of HO-1 were higher in the IL-1beta group than the normal control group (P < 0.05), and there were significant differences between the FDP groups and IL-1beta group. FDP decreased cell apoptotic percentage. Activities of HO-1 and content of CO were higher than that in the IL-1beta group (P < 0.01).</p><p><b>CONCLUSION</b>FDP can attenuate the IL-1beta induced apoptosis of cultured beta cells, the mechanism of which may be improved HO-1 activity and CO content.</p>

Inhibition of the proliferation and invasion of pancreatic cancer cells by MAT1 gene-targeted short interfering RNA in vitro / 肿瘤

Objective: To observe the influence of silencing MAT1 gene by short interfering RNA (siRNA) on the proliferation and invasion of human pancreatic cancer cells and discuss the feasibility of using MAT1 gene as therapeutic target for treatment of pancreatic cancer. Methods: BxPC-3 cells were transfected with sequence-specific siRNA targeting MAT1 gene with liposome mediation. The mRNA and protein expression of MAT1 were confirmed by RT-PCR and Western blot, respectively. The cell proliferation was measured by Trypan blue exclusion staining. The invasion ability was determined by Boyden chamber model in vitro. Results: The mRNA and protein expression of MAT1 were significantly down-regulated by siRNA in BxPC-3 cells compared with the control groups. The expression of MAT1 mRNA was reduced by 55.2% and 64.3% at 24 h and 48 h, respectively (P <0.01). The cell proliferation and invasion ability of BxPC-3 cells were significantly inhibited in vitro (P < 0.01). Conclusion: The results suggest that siRNA targeting MAT1 gene inhibits the cell proliferation and invasion of BxPC-3 cells in vitro. MAT1 may be a potential target for gene therapy of human pancreatic cancer.

Mechanism of alleviation of STZ induced pancreatic islets apoptosis by fructose-1,6-disphosphate / 基础医学与临床

Objective To investigate the protective role of FDP to STZ induced islest apoptosis and the potential mechanisms.Methods The pancreases of the rats were treated to collect islets cells.The cells were incubated with STZ with/or FDP.Cell morphology,insulin secretion,HO-1 activity,CO content,SOD activity,GSH-px activity,iNOS activity were examined.No conetent and apoptotic percentage was detected.Results HO-1 activity and CO content of the normal control group were low.STZ induced a significant decrease of cell activity and insulin release,flow cytometry analysis showed that apoptotic percentage of islet cells remarkably increased following the addition of STZ,FDP obviously improved the islets cellular activity damaged by STZ,basic amount of insulin secretion and stimulated by high glucose were improved(P

Intracytoplasmic sperm injection in cases with history of in vitro fertilization failure / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To evaluate the effect of intracytoplasmic sperm injection (ICSI) in the management of cases with a history of conventional in vitro fertilization (IVF) failure.</p><p><b>METHODS</b>Two groups of patients, 19 with normal semen parameters and a history of IVF failure (metaphase II oocytes: 0-30 %) and 28 with severe male factor infertility received ICSI technology during the same period. Ovarian stimulation was achieved by conventional procedure. Transvaginal ultrasound-guided oocyte collection was done 35-37 h after human chorionic gonadotrophin (hCG) injection. Only metaphase II oocytes were selected for microinjection.</p><p><b>RESULTS</b>Fertilization was achieved with ICSI in all the patients. The fertilization rate (75.6 % +/-21.1 % vs. 73.9 % +/-19.2 %), cleavage rate (85.1 % +/-19.3 % vs. 82.7 % +/-22.1 %), clinical pregnancy rate per embryo transfer cycle (31.6 % vs. 28.6 %) and implantation rate per embryo (15.3 % vs. 14.4 %) did not differ significantly between the two groups.</p><p><b>CONCLUSION</b>ICSI is a valuable method for couples with a history of IVF failure. These patients may have a similar ICSI result as in severe male infertility.</p>

The influence of taurine on IL-1?induced inhibition of insulin release from rat pancreaticislet cells / 中国糖尿病杂志

The effect of taurine on IL-1?induced inhibition of insulin release was investigated with cultured neonatal rat pancreatic islet cells.The results indicated that:①Insulin release, both the basal and glucose-stimulated,could be significantly inhibited by addition of IL-1?(5～20U/ml).②The inhibitory effect of IL-1? on insulin release could be reversed by taurine and this effect was directly related to the dose and action time of taurine.

Effects of taurine on the change of apoptosis induced by IL-1?, TNF-? and IFN-? in rat pancreatic islet cells / 中国病理生理杂志

AIM: To investigate the changes of apoptosis in isolated pancreatic islet cells, insulin secretion, expression of Bcl-xL and Bax induced by combination of IL-1?, TNF-? and IFN-?, and effects of taurine on them. METHODS: Isolated pancreatic islet cells from Wistar rat were incubated in monolayer in vitro. NO-2/ NO-3 production, NOS activity, insulin secretion, the protein expression of Bcl-xL and Bax, percentage of islet cell apoptosis and DNA fragmentation in pancreatic islet cells incubated with combination of IL-1?, TNF-? and IFN-? were measured, and the effects of taurine on the changes of them were further investigated. RESULTS: Combination of IL-1?, TNF-? and IFN-? induced a significant increase in percentage of pancreatic islet cell apoptosis, NO-2/ NO-3 production and NOS activity, DNA ladder appearance, a decrease in insulin content, up-regulation in the protein expression of Bax and down-regulation in the protein expression of Bcl-xL (P