ABSTRACT
Aims: Our study was carried out to appraise the phytochemical screening and antioxidant potentials of Sonneratia caseolaris (Linn.) bark extracts. Study Design: For the purpose of this experiment the extracts were subjected for an in-vitro study. Place and Duration of Study: The study was carried out in August 2014 in the Department of Pharmacy, Southeast University, Dhaka, Bangladesh. Methodology: The various fractions of Sonneratia caseolaris (Linn.) barks as Ethanolic (ETF), ethyl acetate (EAF), chloroform(CLF) and pet ether (PTF) fractions-were obtained after extraction were subjected to preliminary phytochemical screening. The antioxidant capacity of these fractions were evaluated using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay .Total antioxidant activity and total phenolic content of ETF, EAF, CLF and PTF extracts of S. caseolaris were determined. Results: The phytochemical screening showed the presence of flavonoid, steroid, tannin compounds in large amounts. In DPPH scavenging assay among the extracts, ethanolic fractions exhibited the highest radical scavenging activity with IC50 of 4.57 μg /ml .The highest phenolic content was found in EAF extracts (63.00 mg of GAE / g. of dried extract) followed by CLF (36.25 mg of GAE / g. of dried extract) and PTF (26.28 mg of GAE /g. of dried extract). The highest total antioxidant activity was also found in ETF fraction (185 GAE/g of dried sample followed by EAF fraction (99.00GAE/g of dried sample), PTF (84.00 GAE/g of dried sample) and Chloroform (49.00 GAE/g of dried sample). Conclusion: Our result demonstrates that all the extractives of S. caseolaris have appreciable antioxidant activities. But, further study is necessary to isolate the active compounds.
ABSTRACT
Objectives: Youngia japonica (YJA), belonging to the family asteraceae, exhibits strong antiallergic, antioxidant and antitumor activities. The present study was carried out to assess the antioxidant potentials, analgesic, anti-inflammatory and CNS depressant activities of different fractions of YJA plant extracts. Study Design: For the purpose of this experiment the different plant extracts were subjected for an in-vitro and in-vivo study. Place and Duration of Study: The study was carried out on March 2015 in the Department of Pharmacy, Southeast University, Dhaka, Bangladesh. Methods: The antioxidant capacity of ethyl acetate (EA), pet ether (PET) and chloroform(CLF) extracts of YJA were investigated for free radical scavenging activity using DPPH and hydroxyl radical scavenging assay. Total antioxidant activity and total phenolic content of different extracts were determined spectroscopically. Analgesic activity was evaluated by using acetic acid induced writhing, formalin test and eddy’s hot plate method. Extracts of YJA were also investigated for anti-inflammatory activity using carrageenan induced hind paw edema model. The CNS depressant activity was evaluated by hole cross test. Results: In DPPH scavenging assay, CLF exhibited the highest DPPH scavenging activity (IC50 9.70 μg/ml). In case of hydroxyl radical scavenging assay, EA extracts showed the most significant activity (IC50 15.09 µg/ml). This result was in line up with the total phenolic content where EA extracts possessed the highest amount of it (43.92 mg of GAE / gm. of dried extract). Moreover, the highest total antioxidant activity was also found in EA fraction (109.30 GAE/gm of dried sample) that rationalizes the previous outcome. All fractions significantly (p<0.01) reduced the writhing and the number of licking in a dose dependent manner (100 and 200 mg/kg). The Extracts also showed significant (p<0.001) inhibition of carrageenan induced paw edema. A statistically significant (p<0.001) decrease in locomotor activity was also observed. Conclusion: The result demonstrates that the YJA has appreciable antioxidant, analgesic, anti-inflammatory and CNS depressant activities.
ABSTRACT
Aims: The study was carried out to assess the analgesic, anti-inflammatory and CNS depressant activity of the methanolic extract of Abelmoschus esculentus Linn. Seeds. Study Design: The Present study was designed to observe pharmacological activities of the crude extract of the plant Abelmoschus esculentus seeds. The study consisted of hot extraction of the seeds of the A. esculantus with methanol. Afterwards, Methanolic crude extract was filtered and the filtrate was evaporated. Finally, screening of analgesic, antiinflammatory and CNS depressant activity of crude extracts of A. esculantus on Swiss Albino mice. Place and Duration of Study: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh. January, 2013- July 2013. Methodology: The animals are divided into Four groups and each group consists of five mice. Analgesic activity was performed by acetic acid-induced writhing model and formalin induced licking and biting in mice. Anti-inflammatory effects of Abelmoschus esculentus seed extract were done by carrageenan induced anti-inflammatory method at the dose of 100 and 200 mg/kg b.wt., (p.o). The CNS depressant activity was evaluated by observing the reduction of locomotor and exploratory activities in the hole cross and opens field tests at the dose of 100 and 200 mg/kg body weight. Results: In statistical analysis, the dose (200 mg/kg) was found to exhibit (significant p=0.05) better analgesic activity (65.16% and 54.38%) against both acetic acid and formalin induced pain in mice which is about similar to standard drug Indomethacin. The extract of A. esculentus (100 and 200mg/kg) also showed sustained inhibition (54.97% and 65.56%) of paw edema at the 4th hour compared to Indomethacin (74.17%). Besides this A. esculentus (significant p=0.05) seed extract (100 and 200mg/kg p.o.) also possesses depressant activity at 90min in both methods. Conclusion: this study recommends that the methanolic extract of the Abelmoschus esculentus seeds has significant CNS depressant, analgesic and anti-inflammatory properties.