ABSTRACT
Acrylamide [AA] has many applications in the chemical industry. It has been shown to be a reproductive toxicant in animals and is associated with risk of cancer. The objective of this study was to investigate the protective effect of 5-aminosalicylic acid [5-ASA] against AA induced testicular and geno-toxicity. Experimental study. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. Animals were orally gavaged with AA at a dose of 45 mg/kg/day for five consecutive days and 5-ASA was injected concomitantly at two different doses, 25 and 50 mg/kg/day. Effect on epididymal sperm count, on histological changes in the testis, on COMET assay in blood leukocytes, on serum testosterone level and on CYP2E1 expression in liver and testis [S9] fractions. COMET assay undertaken on blood leukocytes showed geno-toxicity in the form of COMET cells with increased tail movement, while ELISA of serum testosterone showed severe reduction in testosterone level, which was reversed by concomitant 5-ASA treatment. ELISA of CYP2E1 showed a two-fold higher concentration in control liver S9 when compared to control testis S9. 5-ASA [50 mg/kg] induced the liver CYP2E1, potentially increasing AA metabolism and clearance. Light microscopy examination showed multinucleated giant cells and tubular atrophy in the testis after AA treatement. At the used dose, AA caused toxic effects in male rat that can be reduced by concomitant treatment with 5-ASA, which might be considered as an antidote to AA toxicity in victims of AA poisoning
ABSTRACT
Acrylamide [AA] has been shown to be a reproductive toxicant in animals and is associated with risk of cancer. The objective of this study was to evaluate the dose-dependent acute testicular toxicity of AA in rats. Experimental study King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia Forty-eight rats Animals were loaded with AA orally at doses [5, 15, 30, 45, 60 mg/kg/day] for five consecutive days Histopathological effects of AA on testis and epididymis AA induced a significant body weight reduction, increase in testis / body weight ratio and a significant reduction in sperm count, in the two groups treated with 45 mg and 60 mg/kg/day. Abnormal sperm shapes were detected in all groups. Histopathological signs of AA toxicity on testes and epididymis included; degeneration of spermatogonia, widening of intercellular junctions and degeneration of peritubular myoid cell. Sertoli cells showed darkening of its nuclei, detachment from the basement membrane, increase in the number and size of lipid droplets in their cytoplasm, failure of sperm release and phagocytosis of some sperms. Leydig cell atrophy was observed which contributed to sperm defects and various abnormal histopathological lesions including apoptosis in rat testis. A possible cause of tail intersegmentation seen in mature sperm tails was clarified by electron microscope [EM]examination. AA induced harmful effects on the testis evidenced by degeneration of spermatogenic and Sertoli cells and Leydig cells atrophy in addition to reducing sperm count and appearance of abnormal sperms