ABSTRACT
Objectives: the goal of this study, is to demonstrate the lethal effect of topical Cedar wood oil [P-methyl -d- 3- tetrahydroacetophenone] administration alone and1 or in combination with the solvent: Demzo [Dimethylsulphoxide], and Propolis [a natural bee wax extract, or resinous substance collected by bees from tree exudates], in experimental schistosomiasis mansoni. It is a trial to explore the repercussions of using these compounds upon worm and tissue egg loads, and oograrn pattern in experimental murine schistosomiasis mansoni
Methods: in this study, a group of 40 Swiss albino mice was used. This group was further subdivided into four small subgroups. Subgroup I: constituted control untreated mice. Infection was done by dermal inoculation of 80 Schistosoma mansoni cercariae per mouse through a ring, after shaving the abdominal skin. Subgroup II constituted abdominally shaved mice, wiped with absolutely 100 ul per mouse Demzo + 800 ul per mouse Cedalwmd oil then infected immediately after with 80 Schistosoma mansoni cercariae. Subgroup III: abdominally shaved mice, wiped with absolutely 100 ug per mouse Proplis + 100 ul per mouse Demzo + 800 ul per mouse Cedarwood oil, then infected immediately after with 80 Schistosoma mansoni cercariae. Subgroup IV constituted mice wiped over the abdominal skin with absolutely 50 ug per mouse Propolis + 100 ul per mouse Demzo+ 800 ul per mouse Cedarwood oil and again, infected immediately after with 80 Schistosoma mansoni cercariae. All animals were sacrificed 6 weeks post infection
Results: mice given absolutely [100 ug per mouse Propolis + 100ul per mouse Demzo + 800 ul per mouse Cedar wood oil], revealed a statistically significant drop [P< 0.001] in the total worm recovery, when compared to the respective untreated controls. This value was less evident in the group given half the dose of Propolis [50 ug], and least evident in the group given Demzo and Cedarwood oil only. Again, the former group revealed the least number of tissue egg load [both hepatic and intestinal], and more mature than immature eggs in the oograrn, compared to the latter two groups
Conclusion : cedarwood oil could be successfully used in conjunction with Demzo and Pro- polis as a topical prophylactic agent in experimental schistosomiasis mansoni infection. This constitutes a hopeful and promising tool in endemic areas. In Egypt, this could be of special interest when indigenous or newly coming foreigners are exposed to unpreventable infection, such as fishing or rowing in the Nile River
ABSTRACT
The goal of this study, is to evaluate the effect of a combination between an anthelmintic drug praziquantel [CAS 55268-74-1 EMBAY 8440, Biltricide] , and a muramyl dipeptide derivative Adamantylamide Dipeptide [AdDP] [CAS 768-94-5 [Amantadine]] in potentially tolerized Schistosoma mansoni infected, egg-injected C57BU6 mice . It is also a trial to elucidate the repercussions of this drug combination upon worm and tissue egg loads and oogram pattern.. A group of 120 C57BU6 mice was used in the experiment. This group was further subdivided into five small subgroups. Subgroup I constituted infected control mice. Subgroup II: received four intravenous doses [10ug each via the tail vein] of soluble egg antigen [SEA] on days -7, -5, -3 and -2 before infection. Subgroup III: included infected mice given AdDP 12 mg subcutaneously in 0.2 ml saline. Subgroup IV: infected mice given Antigen [SEA] + AdDP. Subgroup V: included mice given Antigen [SEA] + AdDP + PZQ [500 mg/Kg for two successive days]. Sacrifice was done 10 weeks post infection. Egg-injected mice given the combination regimen Praziquantel + Adamantylamide Dipeptide [PZQ + AdDP], compared to infected untreated control, revealed absence of worm recovery at perfusion and 100% dead ova in the oogram. Again an evident reduction in the hepatic and intestinal tissue egg loads was recorded in this group compared to infected untreated [wether egg injected or not] control mice