ABSTRACT
Objective: to identify the frequency of the rs143383 SNP in the GDF5 gene, which is located in the 5'-untranslated region of Turkish population with knee osteoarthritis [OA]
Study Design: a case-control study
Place and Duration of Study: orthopedics and Traumatology Department, Bozok University Medical Faculty, Yozgat, Turkey, from 2012 to 2014
Methodology: patients diagnosed with OA [n=94] and patients who did not have joint complaints [n=279] were enrolled in this study. Patients diagnosed with osteoarthritis according to the 1986 American College of Rheumatology osteoarthritis criteria and Kellgren and Lawrence scores were investigated, based on age, gender, and X-ray findings. Blood samples were taken for the identification of GDF5 [rs143383] SNPs by PCR/RFLP, according to a standard protocol
Results: this study included 373 patients. The OA group [25.2%; n=94] was characterized by specific genotypes: TT [39.4%; n=37]; heterozygotes [TC; 45.7%; n=43]; and homozygotes [CC; 14.9%; n=14]. The control group [74.8%; n=279] was comprised of TT [26.5%; n=74], TC [54.8%; n=153], and CC [18.6%; n=52] genotypes. An analysis of rs143383 SNP of the GDF5 gene polymorphism revealed that the rs143383 TT genotype had a higher risk for OA [crude OR=1.798, 95% CI=1.010-2.941, p=0.021]
Conclusion: this study demonstrated that there is a correlation of +104T/C polymorphism in the 5'-UTR of GDF5 with knee OA in a Turkish population
ABSTRACT
To evaluate immunity against measles and its relation with some variables among healthy subjects in 3 cities in Turkey. We carried out a cross-sectional study on measles antibody titers in the serum samples of 712 people from Antalya, 696 from Diyarbakir and 667 from Samsun, Turkey using particle agglutination test between February 2000 and October 2001. The study groups, informed and asked for their consent by midwives, consisted of randomly selected subjects of all ages older than 6 months. We implemented the study in 3 steps: physical examination, interview and blood collection. We considered titers of >/= 1:16 as positive and we observed lower seropositivity in Diyarbakir [90.8%] than Antalya [95.9%], and Samsun [94.9%] [p=0.040]. We also observed that seropositivity was lower among preschool group than older groups [p=0.006]. The number of doses of measles vaccine [p=0.001] and measles infection history [p=0.003] were found as a factor increasing the seropositivity ratio. There was no statistically significant between age groups [p=0.219], gender [p=0.148], residence [p=0.537], and number per household [p=0.983] among the provinces. Based on the findings, measles infection still has a high incidence in Turkey and the second dose of measles vaccine is extremely important in the prevention of measles transmission among school children and the community. Furthermore, we must improve our regional differences in routine vaccination services