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Objective To establish a simple and sensitive method for the determination of plasma drug concentration in rats after intragastrical and intravenous administration of paclitaxel loaded nanoparticles and to evaluate its pharmacokinetic characteristics .Methods AgilentTC-C18 column (250 mm × 4 .6 mm ,5 μm) was used for HPLC at the flow rate 1 ml/min with ketoconazole as internal standard and methanol acetonitrile water (45:20:35) as the mobile phase .The statistical mo-ment method was applied to calculate the pharmacokinetic parameters and to evaluate pharmacokinetic characteristics .Results There was a good liner relationship (r=0 .9997) when rat blood concentration of Paclitaxel ranged from 0 .5 to 20μg /ml .The accuracy and precision were in line with the requirements of biological sample analysis .The t1/2 were 3.86 ,3.76 ,3.35 and 2 .62 h after intravenous injection of three lab-made paclitaxel nanoparticles and paclitaxel solution via rat tail vein .The t1/2 were 5 .28 and 3 .72 h respectively after intragastrical administration of lab-made paclitaxel nanoparticles and paclitaxel suspen-sion .Conclusion This method can be used for the pharmacokinetic study of paclitaxel nanoparticles in rats .The paclitaxel loaded nanoparticles exhibited significantly longer in vivo retention time than paclitaxel injection and paclitaxel suspension .
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Objective To explore the feasibility of bletilla striata polysaccharide as a drug delivery material .Method With hydrophobic cholesterol succinyl bletilla striata polysaccharide (CHSB) as the carrier ,the model drug paclitaxel (PTX) loaded nanoparticles were prepared via dialysis method .The morphology of nanoparticles was observed by Transmission Elec-tron Microscope (TEM) .The particle size ,distribution and zeta potential were characterized by Dynamic Light Scattering in-strument (DLS) .The entrapment efficiency ,drug loading and in vitro release were determined by high performance liquid chromatography (HPLC) .Differential Scanning Calorimetry (DSC) was used to confirm the drug form in the drug loaded nan-oparticles .The in vitro antitumor activity of nanoparticles was assayed by MTT .The uptake of nanoparticles by QGY-7703 liver cancer cells was observed by fluorescence labeling method .Results The prepared nanoparticles had spherical shape with uniform particle size distribution .The drug was loaded in the interior of the nanoparticles .Drug loading and encapsulation effi-ciency were affected by the CHSB in certain range .The cytotoxicity of drug loaded nanoparticles on liver cancer cells was stron-ger than the free drug .The fluorescence of Rhodamine B labeled drug nanoparticles were observed in the cells .Conclusion Cholesterol succinyl bletilla striata polysaccharide (CHSB) is highly feasible as an insoluble drug carrier .It can be used as a po-tential Nano carrier material .
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Objective To explore the related risk factors of aortic esophageal fistula (AEF) after esophageal carcinoma operation.Methods According to the design of case-control study and matching proportion of 1:3,18 cases of AEF after esophageal carcinoma operation treated in the thoracic surgery department of Daping Hospital of Third Military Medical University from 2000 to 2015 served as the observation group,and contemporaneous 54 cases of esophageal carcinoma operation,who had approximate basic diseases or risk factors,were selected as the control group,18 pairs were formed.The risk factors possessed by the observation group before disease onset such as age,sex,tumor TNM stage and tumor location served as the matching variables.The non-matching variables including operation time,preoperative body mass index (BMI),amount of intraoperative hemorrhage,preoperative LDL level,anastomosis mode,postoperative pulmonary complications,postoperative arrhythmia,preoperative hypertension,preoperative diabetes and number of removed lymph nodes were performed the case-control study.Results The univariate Logistic analysis preliminarily screened out 5 risk factors,including preoperative obesity,preoperative LDL level,postoperative pulmonary complications,anstomosis mode and number of removed lymph nodes,the difference in other factors was not statistically significant (P＞ 0.05).The multivariate non-conditional Logistic stepwise regression analysis revealed that preoperative obesity (OR =8.63,95 ％ CI=1.35-17.60),preoperative LDL level (OR =0.81,95 ％ CI =0.23-0.98) and the number of intraoperatively removed lymph nodes (OR =0.32,95 ％ CI =0.14-0.59) had statistical differences between the observation group and control group,but the difference in other factors had no statistical significance.Conclusion Preoperative obesity,preoperative LDL level and number of removed lymph nodes might be the risk factors for AEF occurrence after esophageal carcinoma operation.
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The establishment of negotiation and consultation mechanism between medical insurance management agency and medical institution,could proceed the development of provider payment system reforms.This paper discussed the policy basis and the basic principle of negotiation and consultation in provider payment system reforms,and the subject,level and model,content,the carrying out and so on.
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<p><b>OBJECTIVE</b>To identify the clinical and pathological features of acute myeloid leukemia with B lymphoproliferative disorders.</p><p><b>METHODS</b>The characteristics of 3 cases of acute monocytic leukemia with untreated chronic lymphocytic leukemia/monoclonal B-cell lymphocytosis were reported with literatures review.</p><p><b>RESULTS</b>The patients presented with a history of anemia, bleeding and/or fever. Acute monocytic leukemia was diagnosed by bone marrow morphology, cytochemistry and pathology studies. Immunophenotyping by flow cytometry analysis showed a significant population of absolute B-lymphocyte count of > 5×10(9)/L in a patients, similar to that of chronic lymphocytic leukemia.</p><p><b>CONCLUSIONS</b>The association of acute monocytic leukemia and untreated chronic lymphocytic leukemia/monoclonal B-cell lymphocytosis was a rare event. The abnormal B lymphocytes was likely to be misdiagnosis. Thus, it was important to combine several kinds of laboratory studies, especially flow cytometry to identify this rare disorder.</p>