Effects of stimulation of intact vagus nerve on systemic inflammatory response in rats / 中华烧伤杂志

<p><b>OBJECTIVE</b>To observe the effects of stimulation of intact vagus nerve (IVNS) on systemic inflammatory response in rats.</p><p><b>METHODS</b>The model of systemic inflammatory response was reproduced by lipopolysaccharide (LPS). One hundred Sprague Dawley rats were randomly divided into A group (with intravenous injection of LPS), B group (with stimulation of efferent nerve trunk of vagus nerve after intravenous injection of lipopolysaccharide and vagotomy), C group (with stimulation of intact vagus nerve after intravenous injection of LPS), D group (with vagus nerve stimulation after injection of equivalent isotonic saline), E group (with intravenous injection of equivalent isotonic saline), with 20 rats in each group. Five rats of each group were used to determine mean aortic pressure (MAP) before injection and l0 min, 0.5 h, 1 h, 2 h, 4 h and 6 h after injection. The serum levels of tumor necrosis factor (TNF) alpha and interleukin (IL) 10 were determined with enzyme linked immunosorbent assay before injection and 2, 4 and 6 hour after injection.</p><p><b>RESULTS</b>The level of MAP rose in A, B, C groups at 10 min after injection, especially in A group (134.40 +/- 7.3 mm Hg, l mm Hg = 0.033 kPa, P < 0.05), but it dropped in above groups at 30 min after injection. The level of MAP in A group was obviously lower than that in B, C groups during 10 min -6 h after injection. The serum level of TNF-alpha in A group was significantly higher than that in B, C groups at 2, 4, 6 hours after injection (P < 0.05). Compared with that in C group, the serum level of IL-10 in A, B groups lowered markedly at 4, 6 hours after injection (P < 0.05).</p><p><b>CONCLUSION</b>IVNS can stabilize hemodynamics and exert have anti-inflammatory effects at early stage of systemic inflammatory response.</p>

The initial approach of two sides hypogastric artery ligation and isolation with laparoscope before excision of presacral tumor / 中华外科杂志

<p><b>OBJECTIVE</b>To observe the significance of excision of presacral tumor after two sides hypogastric artery ligation and tissue dissociation with laparoscope.</p><p><b>METHODS</b>Twenty-one patients with sacral tumor were performed excision of presacral tumor after two sides hypogastric artery ligation and tissue dissociation with laparoscope.</p><p><b>RESULTS</b>All sacral tumor were removed successfully, the mean volume of operative blood was 800 ml (range 500-1900 ml), and all the patients were followed up 3-25 months, averaged time 11 months. One patient was recurred after 2 months of operation (the patient was Ewing's sarcoma, and refused to accept radiotherapy and chemotherapy after operation), 1 patents died of brain metastases after 9 months of operations. There were no recurrence in the others patients.</p><p><b>CONCLUSIONS</b>The excision of presacral tumor after two sides hypogastric artery ligation and tissue dissociation with laparoscope is an effective operation method, with the advantages of decreasing the operative blood and difficulty of sacral tumor excision, and diminishing the operation wound.</p>

Changes of sympathovagal balance in endotoxemia rats / 中华创伤杂志

Objective To investigate the changes of sympathovagal balance and the effects of va- gus stimulation on sympathovagal balance in endotoxemia rats.Methods Twenty-four Spragne Dawley rats were randomly divided into four groups.The frequency domain of heart rate variability(HRV)com- ponent was analyzed at 0 min,2 ,4 and 6 hours after intravenous injection of lipopolysaccharide(LPS, 5mg/kg)or physiologic saline,and cervical vagal nerve was stimulated(5mv,2ms,1Hz,5 min lasted, 20 min interval)when LPS or physiologic saline was injected.The levels of Noepinephine(NE)and Ace- tylcholin(ACh)were measured in liver tissues.Results Normalized low frequency(LFnm),hormali- zed high frequency(HFnm),very low frequency(VLF),LF/HF values and liver ACh were significantly increased(P＜0.05)and the level of liver NE was significantly decreased (P＜0.05)after LPS admin- istration.Vagal nerve stimulation markedly increased HFnm but decreased LFnm,VLF,LF/HF values, and the liver ACh also significant increased(P＜0.05 ).Conclusion The results suggest that the ac- tivity of sympathetic and vagal nerve was increased during endotoxemia,but the sympathetic activity was more excitable than that of vagal nerve.Vagal nerve stimulation increased the tone of vagus nerve while the tone of sympathetic nerve was decreased in this study.This may be beneficial for anti-inflammatory activity of vagal nerve.