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Objective To investigate the impact of hepatitis A vaccine (HAV) intervention on clinical manifestations and impulse oscillometry (IOS) pulmonary function in children with asthma.Methods Eighty asthma children in the pediatric department of the Southwest Hospital of Third Military Medical University were selected as the control group,78 children were in the intervention group.The attenuated live HAV was adopted to conduct the intervention.The clinical manifestations and IOS pulmonary function were observed in 6 months and 12 mohths after inoculation.The case control study and analysis were performed.Results The symptom control and partial control rate at 6 months after HAV vaccine intervention in the intervention group was 89.8 ％,which was higher than 86.3 ％ in the control group,but the difference was not statistically significant (P＞0.05).The symptom control and partial control rate at 12 months after intervention in the intervention group was 92.3％,which in the control group was 87.6％,the asthma symptoms control situation in the intervention group was improved markedly,the difference was statistically significant (P＜0.05).The peripheral airway viscosity resistance (R5-R20) at 6 months after HAV vaccine intervention in the intervention group was lower than that in the control group,but there was no statistically significant difference (P＞ 0.05);Zrs,Fres,R5 at 12 months after intervention in the intervention group were lower than those in the control group,the difference was statistically significant (P＜0.05).Conclusion HAV vaccine intervention could improve clinical symptoms control and IOS lung function in children with asthma after one year.HAV vaccination has a protective effect on asthma in children.
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Objective To compare the effect between Saccharomyces boulardii sachets and tetragenous viable bifidobacterium tablets in the treatment of rotaviral gastroenteritis of children .Methods A total of 191 patients (below 2 months to 8 years old) with rotaviral gastroenteritis admitted to this hospital from January 2012 to May 2014 were divided randomly into control groups (smectite ,conventional treatment ,number=62) ,tetragenous viable bifidobacterium tablets group (conventional treatment combined with tetragenous viable bifidobacterium tablets ,number= 64) ,saccharomyces boulardii group (conventional treatment combined with saccharomyces boulardii ,number=65) .Therapeutic effects were compared between 3 groups after 3 days and 5 days of treat‐ment .Results The obvious curative rate ,effective ratio and total rate in the groups of saccharomyces boulardii and tetragenous via‐ble bifidobacterium tablets were higher than those in the control group(P0 .05) .There was significant difference in 3 days and 5 days therapeutic effect among 3 groups (P<0 .05) .The duration of diarrhea in the group of Saccharomyces boulardii was lower than this in the group of tetragenous viable bifidobacterium tablets (P<0 .05) .Conclusion The therapeutic effect of Saccharomyces boulardii is similar to that of tetragenous viable bifidobacterium tablets in treatment of children with Rotaviral gastroenteritis .The duration of diarrhea in the group of Sac‐charomyces boulardii was lower than that of the group of tetragenous viable bifidobacterium tablets .
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Objective To investigate the risk factors which affect complex febrile seizures developing into epilepsy. Methods Retrospective analysis of 162 complex febrile seizure cases was carried out. Results Fifteen out of 35 children who had familial history of epilepsy developed into epilepsy. Twenty out of 55 cases who had neurological abnormalities developed into epilepsy. Nineteen out of 37 cases of who the length between fever and febrile seizure was less than 1 h developed into epilepsy, but only 13 in 125 patients developed into epilepsy when the interval between fever and seizure was more than 1 h. Conclusion The predictive risk factors for complex febrile seizures developing into epilepsies are familial history of epilepsy, abnormal neurological development, and short intervals between fever and febrile seizure activities.
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Objective To evaluate the hepatocyte function and ultramicrostructure of marrow mesenchymal stem cells (MMSCs) derivation hepatocyte like cells. Methods MMSCs were isolated from fetal marrow. MMSCs were cultured and induced in vitro in 1% Matrigel as matrix, 2.5 ?mol/ml azacitidine (AZA) pretreatment for 10～12 h, hepatocyte growth factor (HGF) 10 ng/ml+fibroblast growth factor 4 (FGF4) 10 ng/ml+hepatocyte growth medium (HGM). Albumin (ALB) level in culture supernatant was determined with enzyme linked immunosorbent assay (ELISA), capability for dealing with ammonia into urea was detected with urease method, staining for glycogen of undifferentiated MMSCs and MMSCs derivated hepatocyte like cell at induction days 7, 14, 21 and 28 was conducted with periodic acid Schiff's (PAS) test, ultramicrostructure of MMSCs was observed by electromicroscope. Results Undifferentiated MMSCs did not produce ALB and urea. Following treatment with FGF4 and HGF, ALB and urea production by induced MMSCs increased in a time dependent manner. Glycogen storage was first seen by day 14, and maximum levels were seen after day 28, enhancement of glycogen staining for induced MMSCs showed that hepatocyte like cell had glycogen synthesis capability. Undifferentiated MMSCs possessed the construction features of juvenile cell. Induced MMSCs at day 28 had differentiated and had the polarity construction features of epithelioid cell. Conclusions The induced MMSCs has hepatocyte specific construction and functional features.
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Objective To evaluate the efficacy of a new hybrid bioartificial liver support system in the treatment of severe viral hepatitis. Methods A hybrid liver support system consisting of plasma exchange device, plasma perfusion column (charcoal) and a hollow fiber reactor inoculated with 1?10 9 aggregated human hepatocytes or porcine hepatocytes was developed. Ten patients with severe viral hepatitis were treated using this hybrid system. Results The effective rate of the hybrid bioartificial liver support system was 70% (7/10). The mental status and signs of toxicity of patients were improved markedly. Two patients of coma Ⅲ woke up and grade Ⅱ encephalopathy in 4 patients disappeared after treatment. At the same time, a marked decrease in total serum bilirubin and prothrombin time was noted after treatment. Finally, Three out of 10 patients recovered completely. Two patients were bridged to liver transplantation. Five patients died from the complication of hepatic failure or discharge refused advice due to worsening of disease.Conclusions The evident clinical improvement shows that this artifical liver system has prominant liver support effects for the patients with severe viral hepatitis and liver failure.
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In order to evaluate the acid-hase disorders(ABD)in patients with severe viral hepatitis correctly,274 parameters of blood gas and electrolyte were analyzed.According to the combined analysis on the pre-estimated compensatory formula(PCF).anion gap(AG).and potential bicarbonate and chlorine,there were only 78 times of single ABD(28.5%),101 times of double ABD(36.7%),and 95 times of triple ABD(34.6%),which indicates that multiple ABD was quite common in patients with severe viral hepatitis.Among various forms of ABD.respiratory al-kalosis(222 times and 81.0%)was the commonest.then metabolic alkalosis(188 times and 68.6%).metabolic acidosis(136 times and 49.3%),and respiratory acidosis(20 times and 7.3%)occupied the 2nd.3rd.and 4th position on the list.The ratio between alkalemia and acidemia was 2.43 '?1.The incidences of complications and mortality were significantly higher in patients with mixed ABD than in those with simple ABD.
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In order 10 correctly evaluate acid-base disturbances (ABD) in patients with severe viral hepatitis, 274 measurements of blood gases and electrolytes are analysed by the combination of ABD-PCF, AG, potential bicarbonate and chlorine (Cl~). According to the results of ABD-PCF, 142 times (51.8%) were diagnosed as simple ABD and 132 times (48.2%) as double ABD. When AG, potential bicarbonate and Cl- were used to analyse further, simple ABD was diagnosed 78 times only, double ABD 100 times, triple ABD 94 times, and quartet ABD 2 times only. Increased 64 times (48.5%) of mixed ABD were diagnosed by the combined method. This results suggests that the latter was important for correct evaluation of mixed ABD, especially for triple and quartet ABD. The incidences of complications and mortality were significantly higher in patients with mixed ABD than those with simple ABD.