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1.
Chinese Journal of General Surgery ; (12)1993.
Article in Chinese | WPRIM | ID: wpr-522187

ABSTRACT

Objective To investigate the effect of gastric mucosal blood flow and Na + -K + -ATPase activity on gastric transmucosal potential difference during severe intraperitoneal infection in rats. Methods A rat model of intraperitoneal infection was established by cecal ligation and puncture(CLP). Laser Doppler blood flowmeter was used to examine the gastric mucosal blood flow and electric-physiological recorder was applied for measuring gastric mucosal potential difference. Assay of Na + -K + -ATPase activity in gastric mucosal tissue was conducted by biochemistry method. Results Gastric mucosal blood flow(GMBF,mV) in infected group was significantly lower than in control group(43.7?2.8 vs. 57.9?2.7,P

2.
Journal of Third Military Medical University ; (24)1988.
Article in Chinese | WPRIM | ID: wpr-678144

ABSTRACT

Objective To study the association of abdominal cavity metastasis of colorectal cancer with the expressions of CK20 mRNA, CD44v6 and P53 and the related significance. Methods CK20 mRNA in peritoneal fluid was detected by reverse transcription polymerase chain reaction (RT PCR) and proteins of CD44v6 and P53 in cancer tissues were examined immunohistochemically in 50 cases of colorectal cancer. Results Significant correlation was found between CK20 mRNA and the expressions of CD44v6 and P53 in cancer tissues. Conclusion The detection of CK20 mRNA in peritoneal fluid plus examination of CD44v6 and P53 in cancer tissues are helpful for the prediction of abdominal cavity metastasis of colorectal cancer and post operation treatment.

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