ABSTRACT
Objective: The aim of this study was to assess the impact of resveratrol [RST] on oxidative stress induced by methotrexate in rat ileum tissue
Materials and Methods: Twenty-four rats were divided into 4 groups with 6 in each group. Each rat was orally administered the following every day for 30 days: group 1 [MTXG], methotrexate [MTX; 5 mg/kg]; group 2 [RMTXG], MTX [5 mg/kg] plus RST [25 mg/kg/day]; group 3 [RSTG], RST alone [25 mg/kg/day], and group 4 [controls], distilled water. After the rats had been sacrified, the ilea were removed for the assessment of malondialdehyde [MDA], total glutathione [tGSH] and glutathione peroxidase [GSH-Px]. Gene expression analyses for interleukin-1beta [IL-1beta], tumor necrosis factor-alpha [TNF-alpha] and myeloperoxidase [MPO] were also performed. Hematoxylin and eosin-stained paraffinembedded sections of the ileum were analyzed under a light microscope and the findings were recorded. Statistical analyses of the data were performed using one-way ANOVA
Results: The administration of MTX in group 1 yielded a higher level of MDA [8.33 +/- 2.5 micro mol/g protein, p < 0.001] and lower levels of tGSH [0.97 +/- 0.29 nmol/g protein] and GSH-Px [5.22 +/- 0.35 U/g protein, p < 0.001] compared to the other groups. MTX also increased IL-1beta [40.33 +/- 5.43 gene expression levels], TNF-alpha [6.08 +/- 0.59] and MPO gene expression [9 +/- 1.41] in group 1 compared to the controls [11.33 +/- 2.07, 2.15 +/- 0.33 and 3.43 +/- 0.48, respectively, p < 0.001]. The impact of RST on IL-1beta, TNF-alpha and MPO gene expression induced by MTX was observed as a reversal of these findings [p < 0.05]. Severe inflammation, damage to the villus epithelium and crypt necrosis was observed histopathologically in the MTXG group, whereas only mild inflammation was seen in the RMTXG group
Conclusion: In this study, ileal damage caused by MTX was inhibited by RST