Effects of Baduanjin combined medication on cardiac rehabilitation in patients with acute myocardial infarction / 心血管康复医学杂志

Objective :To explore effects of Baduanjin combined medication on cardiac rehabilitation in patients with acute myocardial infarction (AMI).Methods :A total of 106 AMI cases treated in our hospital were enrolled .Ac-cording to random number table ,they were randomly and equally divided into medication group (received Tongxin-luo based on routine treatment ) and combined treatment group (received Baduanjin exercise based on medication group) ,both groups were treated for four weeks .Left ventricular end-diastolic volume (LVEDV) ,left ventricular end-systolic dimension (LVESd) ,left ventricular ejection fraction (LVEF) ,levels of tumor necrosis factor (TNF)-α ,high sensitive C reactive protein (hsCRP) ,interleukin (IL)-6 and N terminal pro brain natriuretic peptide (NT-proBNP) were observed and compared between two groups .Results : Total effective rate of combined treatment group was significantly higher than that of medication group (92.45% vs.75.47%,P＝0.017).Compared with be-fore treatment ,after treatment , there were significant rise in LVEDV and LVEF , and significant reductions in LVESd ,levels of hsCRP ,TNF-α ,IL-6 and NT-proBNP in two groups (P＜0.05 or ＜0.01).Compared with medi-cation group after treatment ,there were significant rise in LVEDV [ (153.58 ± 25.17) ml vs .(165.27 ± 25.46) ml] ,LVEF [(51.43 ± 4.28)% vs.(55.37 ± 4.26)% ] ,and significant reductions in LVESd [(33.46 ± 2.24) mm vs. (30.29 ± 2.32) mm] ,levels of hsCRP [ (4.54 ± 1.12) mg/L vs.(3.48 ± 1.05) mg/L] ,TNF-α [ (117.85 ± 15.46) ng/L vs.(96.59 ± 14.23) ng/L] ,IL-6 [ (14.38 ± 3.34) pg/ml vs.(10.67 ± 3.29) pg/ml] ,NT-proBNP [ (456.29 ± 53.76) ng/L vs.(368.49 ± 53.65) ng/L] in combined treatment group ,P＜0.05 or ＜0.01. Conclusion :Baduan-jin combined Tongxinluo capsule can effectively improve cardiac function ,reduce levels of inflammatory factors and improve therapeutic effect in patients with acute myocardial infarction .

Effects of shRNA targeting PRL-3 on proliferation,invasion and apoptosis of human colorectal cancer cells / 天津医药

Objective To investigate the effects of PRL-3 shRNA mediated by lentivirus on proliferation, invasion and apoptosis of human colorectal cancer SW480 cells. Methods There were three experimental groups in this study, which included blank control group, negative control group and transfected group. Colorectal cancer SW480 cells were transfected with lentiviral interference vector carrying PRL-3 shRNA to build a stable PRL-3-silencing cell line. The expression of PRL-3 mRNA was detected by real-time quantitative polymerase chain reaction (real-time PCR). Cell proliferation was analyzed by MTT method and colony formation assay.Invasion and migration were measured by Transwell assay and invasion chamber. Apoptosis rate was performed by flow cytometry. Results The stable PRL-3-silencing cell line was successfully constructed.Compared with the blank control group and negative control group,the relative expression levels of PRL-3 mRNA were reduced in transfected group after transfection with PRL-3 shRNA(P<0.05),but there was no significant difference between the blank control group and the negative control group.After transfection with PRL-3 shRNA for 72 h,the proliferation of SW480 cells was significantly lower in transfected group than that of the blank control group and the negative control group,and the proliferation decreased significantly in 120 h(P<0.05).Compared with the blank control group and negative control group, the ability of colony formation was also weakened in the transfected group (P<0.05). Compared with the blank control group and negative control group,the migration and invasion ability were decreased in the transfected group(P<0.05),and the apoptosis rate was increased in the transfected group(P<0.05).Conclusion PRL-3 shRNA can inhibit the expression of PRL-3 and the proliferation, promote the apoptosis of SW480, which indicates that PRL-3 may become a target for colorectal carcinoma treatment.

The compliance assessment of endocrine therapy for breast cancer survivors:a review / 中国实用护理杂志

Breast cancer is one of the most common cancers in China. With increasing of the incidence and mortality, the harm to women is becoming much serious. Estrogen positive patients need to take 5-10 years′ endocrine drugs to prolong survival and prevent recurrence. Endocrine therapy has become one of the most common and effective methods in the treatment of breast cancer. In order to achieve the desired effect of cancer treatment, reduce cancer metastasis and recurrence rate, good compliance is essential. In conclusion, this review summarizes the evaluation of compliance, various evaluation tools and their use.

Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells / 中国结合医学杂志

<p><b>OBJECTIVE</b>To explore the neuroprotective effects of baicalin against hypoxia and glucose deprivation-reperfusion (OGD/RO)-induced injury in SH-SY5Y cells.</p><p><b>METHODS</b>SH-SY5Y cells were divided into a control group, a OGD/RO group, which was subject to OGD/RO induction; and 3 baicalin groups subject to baicalin (1, 5, 25 μmol/L) for 2 h before induction of OGD/RO (low-, medium-, and high-dose baicalin groups). Cell viability was detected by thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometric analysis was used to detect cell apoptosis. Real-time polymerase chain reaction was performed to determine the mRNA expression of caspase-3 gene. Western blot analysis was conducted to determine the expression of nuclear factor (NF)-κB and N-methyl-daspartic acid receptor-1 (NMDAR1).</p><p><b>RESULTS</b>Baicalin could significantly attenuate OGD/RO mediated apoptotic cell death in SH-SY5Y cells; the apoptosis rates in the low-, medium- and high-dose groups were 12.1%, 7.9%, and 5.4%, respectively. Western blot and real-time PCR analysis revealed that significant decrease in caspase-3 expression in the baicalin group compared with the OGD/RO group (P<0.01). Additionally, down-regulation of NF-κB and NMDAR1 was observed in the baicalin group compared with those obtained from the OGD/RO group. Compared with the low-dose baicalin group, remarkable decrease was noted in the medium- and high-dose groups (P<0.01).</p><p><b>CONCLUSION</b>Baicalin pre-treatment attenuates brain ischemia reperfusion injury by suppressing cellular apoptosis.</p>

Clinical research of safflower injection on hibernating myocardial revascularization / 中国中药杂志

Coronary artery disease (CAD) is one of the leading causes of death. Safflower attracts great attention owing to its anti-ischemia/reperfusion injury effect. Ninety-three patients with CAD were included and randomized into safflower treatment group, PCI group and control group. Low-dose dobutamine stress echocardiography (DSE) was performed to measure end-systolic volume (ESV), end-diastolic volume (EDV), left ventricular ejection fraction (LVEF) and wall motion score index (WMSI) to determine the recovery of hibernating myocardium and cardiac function in all patients before treatment and after 3-month follow-up. The study was to investigate the effects of safflower on hibernating myocardial revascularization and cardiac function. It was found that LVEF was significantly improved, while the ESV and WMSI were significantly reduced after 2-week treatment in safflower and PCI treatment groups. No significant differences were found between safflower and PCI treatment groups in ESV, EDV, WMSI and LVEF after treatment Safflower injection effectively improved hibernating myocardial function.

Comparison of biological behavior between triple-negative breast cancer and non-triple- negative breast cancer / 南方医科大学学报

<p><b>OBJECTIVE</b>To summarize the epidemiological and biological features of triple-negative breast cancer (TNBC) and non-triple-negative breast cancer (non-TNBC) to provide reference for devising individualized therapy and making prognostic evaluation.</p><p><b>METHODS</b>The 5-year follow-up data were collected from 231 patients with pathologically established diagnosis of breast cancer treated in the Affiliated Hospital of Ningxia Medical University and Yinchuan People's Hospital between Jan. 2002 and Dec. 2004. The epidemiological and clinicopathological characteristics as well as the recurrence, metastasis and 5-year survival were compared between TNBC group and non-TNBC group.</p><p><b>RESULTS</b>TNBC accounted 17.3% of the total breast cancer cases enrolled in this study. The tumor size and rates of recurrence and metastasis (especially visceral metastasis) were significantly greater in TNBC group than in non-TNBC group (P<0.05). The TNBC patients showed significantly lower 3- and 5-year survival rates than the non-TNBC patients (P<0.05), and TNBC patients with positive lymph nodes in clinical stage II had also a lower 5-year survival (P<0.05). Cox regression model analysis identified the patients' age, primary tumor size, clinical stages and triple-negativity as the independent risk factors for breast cancer.</p><p><b>CONCLUSION</b>Compared to non-TNBC patients, patients with TNBC have higher rates of local recurrence and invasion, visceral metastasis and poorer prognosis, and a lower rate of 5-year survival. The triple negativity represents an independent factor for prognosis evaluation of breast cancer.</p>

The Application of Molecular Marking Technology in Diversity Research of Lactic Acid Bacteria / 微生物学通报

This paper outlines some molecular marking technology based on rDNA sequences and several DNA fingerprinting technology (RAPD, ARDRA, AFLP, REP/ERIC-PCR) used in classification, identifica-tion and diversity research in lactic acid bacteria. The principles, methods and progress in recent years of these technologies were also introduced. At the same time, this paper also compares the advantages and dis-advantages of these methods. People should choose suitable method according to their purposes.