Analysis on Application of Salviae Miltiorrhizae Radix et Rhizoma Related Health Food / 中国实验方剂学杂志

With the improvement of living standard and enhancement of health consciousness, Salviae Miltiorrhizae Radix et Rhizoma, as a common medicinal material that can be widely used in health food, is focused an increasing number of scholars′ attention at home and abroad. Based on Salviae Miltiorrhizae Radix et Rhizoma health food published by the food website of National Medical Products Administration (NMPA), this paper combs and analyzes their dosage forms, functional components, health-care functions and prescriptions of traditional Chinese medicine (TCM). Meanwhile, the Apriori algorithm module in SPSS Modeler 15.0 is used to explore the formulation rules of Salviae Miltiorrhizae Radix et Rhizoma health food. The results showed that capsules was the most common (about 55.34%), and the infusions and beverages were rare. In the aspect of health-care function, it is mainly used to auxiliary hypolipidemic, auxiliary protective function to chemical liver injury, enhance immunity and dispel chloasma. Among 92 cases of the health food with auxiliary hypolipidemic, the common combination of TCM was Salviae Miltiorrhizae Radix et Rhizoma-Gynostemmatis Pentaphylli Herba-Puerariae Lobatae Radix. Among 55 cases of the health food with auxiliary protective function to chemical liver injury, the common combination of TCM was Salviae Miltiorrhizae Radix et Rhizoma-Puerariae Lobatae Radix-Schisandrae Chinensis Fructus. Among 54 cases of the health food with immune-enhancing, the common combination of TCM was Salviae Miltiorrhizae Radix et Rhizoma-Astragali Radix-Lycii Fructus. Among 46 cases of the health food with dispelling chloasma, the common combination of TCM was Salviae Miltiorrhizae Radix et Rhizoma-Angelicae Sinensis Radix-grape seeds. The selection of prescription compatibility of Salviae Miltiorrhizae Radix et Rhizoma health food with different health-care functions is basically consistent with the TCM treatment with syndrome differentiation theory and modern medical theory. This article interprets the application of Salviae Miltiorrhizae Radix et Rhizoma health food from the perspective of dosage forms, functional components, health-care functions and compatibility of prescriptions, which can provide a basis and reference for precise and efficient research and development of this kind of health food.

Research progress on functional soft candy of traditional Chinese medicine / 中国中药杂志

Functional soft candy of traditional Chinese medicine( TCM) can be used to supplement vitamins,enhance immunity,assist in lowering blood sugar,assist in lowering blood lipid,assist in lowering blood pressure,relax the bowel,reduce weight and fight againstoxidation.It has attracted much attention due to its effective improvement in the bad taste and incompatibility of traditional medicines.TCM functional soft candy and health food made by gel,sweetener and Chinese herbal medicines( which can be used both as food and medicine) approved by the State Food and Drug Administration were selected as the research objects in this paper.We combed and analyzed the research situation of health food dosage forms and effects,the functional factors of TCM functional soft candy,gelatin,sweetener,production process,and quality evaluation,etc.,and then,we discussed their existing problems and development prospects.After reviewing the relevant literature on TCM functional soft candy and health food researches published in recent years,we analyzed and summarized their dosage forms,health effects,gels,sweeteners,production process,quality evaluation and safety.The following problems in the research of TCM functional soft candy were pointed out: for examples,differences in the sensory evaluation of soft candy;whether the combination of several similar gels and sweeteners had an effect on human health; the lack of laws and regulations in health foods and the lack of uniform standards for quality evaluation.In summary,TCM functional soft candy is a new type of health food,which changes the dosage form of health food based on capsules and tablets.This move conforms to the physiological and psychological needs of consumers.Therefore,it is in line with the requirement of " Health China 2030" Planning Outline.

In vivo antimalarial activity of synthetic hepcidin against Plasmodium berghei in mice / 中国天然药物

The present study was designed to investigate the antimalarial activity of synthetic hepcidin and its effect on cytokine secretion in mice infected with Plasmodium berghei. The mice were infected with P. berghei intravenously and treated with hepcidin according to 4-day suppression test and Rane's test. The serum levels of interleukins (IL-1β, IL-2, IL-6, IL-10, IL-12p70, and IL-17A), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in the experimental mice were determined using a cytometric bead array (CBA) kit. The survival rate of the infected mice was also registered. Additionally, the serum iron, alanine transaminase (ALT), aspartate transaminase (AST), and total bilirubin (BIL) were detected to evaluate liver functions. Hepcidin exerted direct anti-malarial function in vivo and increased survival rate in a dose-dependent manner. In addition, the secretion of T helper cell type 1 (Th1), Th2, and Th17 cytokines, TNF-α, and IFN-γ were inhibited by hepcidin. In conclusion, our results demonstrated that synthetic hepcidin exerts in vivo antimalarial activity and possesses anti-inflammatory function, which provides a basis for future design of new derivatives with ideal anti-malarial activity.

Research of SOCS1 silent DC vaccine on laryngocarcinoma therapy / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#Investigate the specific antitumor mechanism of SOCS1 silent DC vaccine and discuss the prospect of RNAi in the gene therapy for laryngocarcinoma in order to provide novel ideas of DCs clinical applications.@*METHOD@#Dendritic cells derived from peripheral blood monocytes were cultured in vitro in the presence of GM-CSF, IL-4 and TNF-alpha. The morphological feature of DC was observed with inverted microscope. RNAi vector were transfected into DC. The expression of SOCS1 protein was detected with Western blot. The effective target sequences of siRNA against SOCS1 were screened out. The surface markers of mature DC, including CD83, CD86 and HLA-DR, were detected with flow cytometry. The concentration of IFN-gamma in the supernatant was assayed by ELISA. The proliferative ability of T cell stimulated by DC and the specific killing activity of cytotoxic T lymphocyte (CTL) induced by DC were evaluated by MTT assay.@*RESULT@#Dendritic cells were obtained successfully. The RNAi vector was proved to be right by sequencing. The expression of SOCS1 decreased significantly under the influence of the 5th interference sequence. SOCS1 silent dendritic cells which were loaded with Hep-2 antigen had high expressions of CD83 (85.61 +/- 0.96)%, CD86 (96.86 +/- 1.20)% and HLA-DR (98.02 +/- 0.94)%. It could also stimulate the proliferation of T cells effectively as well as could increase the production of IFN-gamma, eventually enhanced the specific killing effect of CTL. The killing activity was more higher than that in control group when the effect cells and target cells were mixed up at the ratio of 50:1 (P < 0.01).@*CONCLUSION@#SOCS1 silent DC vaccines which were loaded with Hep-2 antigen could induce effective and specific anti-laryngocarcinoma immune responses.