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Journal of Zhejiang Chinese Medical University ; (6)2006.
Article in Chinese | WPRIM | ID: wpr-565594

ABSTRACT

[Objective]To observe the indexes of quality control of nature and quantity of Yanluotong Infusion.[Method]Make authentication to the 2 main components of Ligusticum wallichii and rhizoma sparganii on their nature with Silica gel G thin-layer chromatography;make content measurement to puerarin with RP-HPLC,take Kromasil 100-5 C18(4.6mm?250mm,5?m)as chromatogram column,methanol-water(30:70)as flow phase;the measured wavelength is 250nm.[Result]The puerarin sampling in the range 0.06~0.65?g is in good linear relationship with the summit area,the relative coefficient r=0.9999,the intraday and days nicety RSD are respectively 0.85% and 0.48%(n=5);the average sample reclaim rates of high,middle and low concentrations(n=3)are respectively 101.2%,101.1% and 101.4%,RSD is 1.1%,0.94% and 0.41% separately.[Conclusion]Silica gel G thin-layer chromatography and RP-HPLC are correct and reliable for nature and quantity control,with good repeatability,and can be taken as quality control standard of the preparation.

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