ABSTRACT
<p><b>OBJECTIVE</b>To obtain transgenic Pinellia ternata plants resistant to fungus by transfer Chitinase and beta-1,3-Glucanase gene from Trichoderma harzianum.</p><p><b>METHOD</b>Using hygromycin phosphotransferase as the selection marker, the Chitinase gene (ech42), beta-1,3-Glucanase gene (gluc78) and both gene pCAMBIA(ech42 + gluc78) driven by CaMV35S promoter were transferred into P. ternata callus via Agrobacterium-mediated transformation.</p><p><b>RESULT</b>PCR results confirmed that the regenerants were identified to be transgenic lines and the RT-PCR results confirmed that foreign genes construction were transfer to mRNA. Two foreign genes were inherited stably to T5 generation according to PCR results of the lines.</p><p><b>CONCLUSION</b>The results showed that chitinase gene ech42 and beta-1, 3-glucanase gene gluc78 respectively or together introducing and co-integrating into P. ternata</p>
Subject(s)
Agrobacterium tumefaciens , Genetics , Metabolism , Chitinases , Genetics , Metabolism , Fungal Proteins , Genetics , Metabolism , Gene Expression Regulation, Plant , Gene Transfer Techniques , Genetic Vectors , Genetics , Metabolism , Glucan 1,3-beta-Glucosidase , Genetics , Metabolism , Pinellia , Genetics , Metabolism , Transformation, Genetic , TrichodermaABSTRACT
AIM: To investigate the effect of Shenmai Injection on the expression of bFGF and PCNA of gastric cancer in mouse. METHODS: RT-PCR was used to measure the expression of bFGF and PCNA in gastric cancer cell cultured with Shenmai Injection in three concentrations.immunohistochemistry used for protein synthesis in mouse gastric cancer model. RESULTS: 140 ?L/mL of Shenmai Injection concentration inhibited 63% of bFGF and PCNA gene expression. CONCLUSION: Shenmai Injection can inhibit the express of bFGF and PCNA.