ABSTRACT
The present study reports the chemical composition, antioxidant, antibacterial, antidiarrheal as well as the spasmolytic activity of Zanthoxylum rhetsa [DC] essential oil, fractionated oil and its principal constituent [Terpinen-4-ol]. The constituents of essential oil were characterized by GC-FID and GC-MS. The pharmacological and biological activities of oil, its fraction and principal constituent were carried out in-vivo and in-vitro. The oil was rich in a group of monoterpene family, constituted mainly of terpinen-4-ol [25.43%], sabinene [16.50%], beta-pinene [10.4%], alpha-Terpineol [7.63%], gamma-Terpinene [5.64%], alpha-pinene [4.33%], and linalool [3.25%]. The antioxidant capacities of the oil, fractions and terpinen-4-ol were assessed by using spectrophotometry to measure free radical scavenger 2,2-diphenyl-1-picrylhydrazyl [DPPH]. Furthermore, the oil, its fractions and terpinen-4-ol exhibited appreciable antioxidant, antibacterial, antidiarrheal and non-selective spasmolytic activity. The study suggests that the oil and its main active constituent [terpinen-4-ol] of the studied plant would have high potential in the treatment of stress and gastrointestinal diseases
ABSTRACT
A simple, sensitive, and inexpensive high performance liquid chromatographic method has been developed for simultaneous determination of ofloxacin and ornidazole in pharmaceutical formulations. Chromatographic separation was achieved on a BDS-C[18]-Hypersil column [250 mm x 4.6 mm i.d., 10 microm]. Mobile phase was 80% water [containing 0.55 ml/L of triethylamine as peak modifier] and 20% acetonitrile; final pH was adjusted to 3.0 with orthophosphoric acid. Detection was done at 284 nm. Response was a linear function of concentration in the range 1-20 micro g/ml for ofloxacin and 2.5-50 microg/ml for ornidazole; the correlation coefficients were 0.9998 and 0.9995, respectively. The limit of detection were 0.01 and 0.02 microg/ml for ofloxacin and ornidazole respectively, where as limit of quantitation were 0.05 and 0.1 micro g/ml. The accuracy result for ofloxacin and ornidazole at eighty percent drug [80%], hundred percent [100%], and one hundred and twenty percent [120%] were ranged from 99.6-100.9%. The inter- and intra-day precision was less than 1%. Total elution time for the two components was less than 9 min