ABSTRACT
Objectives: To determine the most effective method of sterilization and disinfection of extracted human teeth foruse in dentalcolleges
Study Design: Case Control study
Place and Duration of Study: This study was conducted at OMFS and Microbiology Department, KMDC, Karachi from June 2013 to December 2013
Materials and Methods: Freshly extracted human teeth [n=50] were obtained and sent for bacteriological processing. Teeth were dividing into 5 groups; samples were taken pre and post treatment. A platinum wire loop was flamed in red heat and cooled; sample was inoculatedinC for 48 hours. Colony count was noted to observe the quantity of microorganism, which determines the efficacy of the sterilizing method
Results: The results of the study revealed that the autoclave, hot air oven had shown no growth.While5% sodium hypochlorite, hydrogen peroxide and normal saline had shown positive growth of microorganisms
Conclusion: Autoclave and Hot air oven are effective methods of sterilization of extracted human being teeth for use in dental college in preclinical settings