Kinetic behaviour of proteolytic enzymes on yeast protein

The maximum velocity of the coupling reaction of pepsin and pancreatin on cell lysis higher for a C. utilis mutant, strain, being 0.68 and 1.0 for pepsin and pancreatin respectively. The enzyme velocity constant [k] for the C. utilis mutant was 0.0229 for pepsin and 0.278 for pancreatin. The high digestibility of this strain was due to its low glucan content [1.6%] compared with other strains. A higher levels of lysin was recorded for C. Utilis mutant [5.02% DW]. Methionine content of the mutant was higher than the parent [0.91% to 0.82% DW, respectively]