ABSTRACT
In this study zero, first and second order derivative spectrophotometric method were developed for the estimation of sitagliptine. In zero order spectrophotometery, absorbance value was measured at 267nm. In first derivative spectrophotometry amplitudes were measured at 213nm. In second derivative spectrophotometry amplitudes were measured at 276nm. Calibration curves were linear between the concentration range of 20-60μg/ml, 20-60μg/ml and 40-80μg/ml respectively. The % RSD value is less than 2% and the recovery were near 100% for all methods. This method has been validated for linearity, accuracy and precision and found to be rapid, precise, accurate and economical and can be applied for routine estimation of sitagliptine in solid dosage form. The validation of method was carried out utilizing ICH-guidelines.
ABSTRACT
A simple, rapid and accurate RP-HPLC method was developed for the determination of levosalbutamol in pure and tablet dosage form by RP-HPLC method using C18 BDS column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of Acetonitrile and buffer in the ratio of 20:80 (v/v) was used and maintain the pH 3. The flow rate was maintained at 1 mL/min and the injection volume was 20 μL . Detection wavelength with UV detector at 276 nm and run time was kept 10 min. The retention time of levosalbutamol was 5.4 min. The method was linear over the concentration range 7-12 μg/ml. The recovery was found to be 100.44± 0.27%. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations.
ABSTRACT
A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of ibuprofen and famotidine in tablet dosage forms using C18 column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of Methanol: Water: Phosphate buffer in the ratio of 70:20:10 (v/v/v). The flow rate was 1.0 ml/min and detection wavelength was carried out at 284 nm. The retention times of ibuprofen and famotidine were 3.6 min and 7.8 min, respectively. The method was linear over the concentration range for ibuprofen 2-10 μg/ml and for and famotidine 2-10 μg/ml. The recoveries of ibuprofen and famotidine were found to be in the range of 99.037-100.766% and 99.703-100.433% respectively. The validation of method was carried out utilizing ICHguidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.