ABSTRACT
El libro resalta que la lepra continúa siendo una enfermedad presente en Colombia y que aún constituye un problema de salud pública importante por los costos sociales, económicos y de sufrimiento humano que conlleva. Sabiendo que la literatura sobre el tema es escasa en nuestro medio, este libro surge como una herramienta de consulta creada para médicos y otros profesionales de salud, con la certeza de que es preciso mejorar la oportunidad del diagnóstico. Siendo fundamental que, durante su proceso formativo, todos los profesionales de la salud adquieran conocimientos sobre dicha enfermedad, que cada día se hace más visible por sus secuelas y diagnóstico tardío.
The book highlights the fact that leprosy continues to be a disease present in Colombia and that it is still a major public health problem due to the social, economic and human suffering costs it entails. Knowing that the literature on the subject is scarce in our country, this book is intended as a reference tool for doctors and other health professionals, in the knowledge that it is necessary to improve the timeliness of diagnosis. It is essential that, during their training process, all health professionals acquire knowledge about this disease, which is becoming more and more visible every day due to its sequelae and late diagnosis.
Subject(s)
Humans , Animals , Male , Female , Child , Colombia , Leprosy , Epidemiology , Leprosy/classification , Leprosy/genetics , Leprosy/history , Leprosy/pathology , Leprosy/epidemiology , Mycobacterium lepraeABSTRACT
En este trabajo se investigó la presencia de las principales proteínas de unión a IGF-I en tejidos de rata y el tamaño de los complejos formados en cada caso. Mediante análisis de Western ligand blot (WLB) y cromatografía de exclusión molecular, se separaron e identificaron cuatro proteínas de unión, correspondientes a las IGFBP-1 a IGFBP-4. En todos los tejidos analizados, se detectó la proteína IGFBP-3, aunque su concentración varió con el tipo de órgano, siendo muy abundante en hígado, riñon y corazón y escasa en órganos linfoides (timo y bazo). IGFBP-1 e IGFBP-2 se identificaron en hígado y pulmón y, además, en corazón y músculo esquelético, órganos donde hasta el momento no se han identificado los correspondientes mARN, como tampoco IGFBP-4, identificada en este trabajo en pulmón. En conclusión, los resultados obtenidos indican un perfil típico para las IGFBP de acuerdo con el tejido, aunque no es claro aún si su presencia es el resultado de su síntesis in situ o de un transporte transcapilar. La identificación de sus mensajeros es un paso esencial para comprender el papel regulador que estas proteínas desempeñan en la biodisponibilidad del IGF-I en los diferentes tejidos
Subject(s)
Rats , Insulin-Like Growth Factor Binding Proteins/analysis , Blotting, Western/statistics & numerical dataABSTRACT
In order to evaluate the presence of specific IgG antibodies to Borrelia burgdorferi in patients with clinical manifestations associated with Lyme borreliosis in Cali, Colombia, 20 serum samples from patients with dermatologic signs, one cerebrospinal fluid (CSF) sample from a patient with chronic neurologic and arthritic manifestations, and twelve serum samples from individuals without clinical signs associated with Lyme borreliosis were analyzed by IgG Western blot. The results were interpreted following the recommendations of the Centers for Diseases Control and Prevention (CDC) for IgG Western blots. Four samples fulfilled the CDC criteria: two serum specimens from patients with morphea (localized scleroderma), the CSF from the patient with neurologic and arthritic manifestations, and one of the controls. Interpretation of positive serology for Lyme disease in non-endemic countries must be cautious. However these results suggest that the putative "Lyme-like" disease may correlate with positivity on Western blots, thus raising the possibility that a spirochete genospecies distinct from B. burgdorferi sensu stricto, or a Borrelia species other than B. burgdorferi sensu lato is the causative agent. Future work will focus on a survey of the local tick and rodent population for evidence of spirochete species that could be incriminated as the etiologic agent
Subject(s)
Humans , Antibodies, Bacterial/blood , Borrelia burgdorferi/immunology , Immunoglobulin G/blood , Lyme Disease/etiology , Blotting, Western , Colombia , Lyme Disease/blood , Lyme Disease/immunology , Scleroderma, Localized/complicationsABSTRACT
It is well established that nutrition is an important regulator of both serum insulin-like growth factor-I (IGF) and its binding proteins (IGFBPs). The Western ligand blot method (WLB) for simultaneous determinations of IGFBPs in serum or plasma sambples was evaluated and validated with emplasis on its reproducible capabilities. After electrophoretic separation and transfer, the membranes were incubated with a mixture of recombinant labeled human (GF-I/IGF-II(rhIGF-II) and band intensities measured by autoradiography. The typical electrophoretic profile for pig serum, as determined with molecular weight markers, showed four mainbands of approximately 42-39,32,30-28 and 24 kDa which seemed to correspond to IGFBP-3, IGFBP-2, IGFBP-1 and IGFBP-4 respectively. Likewise, a trinlet of approximately 42-39 kDa (IGFBP-3), a broad area called OGFBP-30 region (most probably IGFBP-1, -2 and -3 variants) and a third band of 24 kDa IGFBP-4) were seen in rat samples. Determination of IGFBP/2 and 1 in rat serum samples, as two separate bands on 12 por ciento gels was difficult due to their close electrophoretic migration and possibly to the reported lower levels of IGFBP-2 in adult rat serum. Dilutions tested on 0.2 µm nitrocellulose membranes with samples volumes between 0.25 to 1.5 µl(1:10-1:60 dilutions), showed IGFBPs curves with good linearity which suggest first, that there exist a quantitative relation betweem the amount of each protein and densitometric response and second, that the transfer of the proteins was linear across the range of 0.25 to 1.5 µl(1:10-1:60 dilutions). Moreover, the results also suggest that losses were aqually spread and that the proteins retained their binding pro[erties after the process. Reproducibility showed intra-assay coefficients of variation (CVs) of 15 per cent or lower using either a transfer device without cooling system or a combination of a transferdevice with cooling system and manually defined band boundaries. In summary, it was shown that the optimized experimental conditions here described for the WLB method, allow realiable simultaneous measurements of the main pig and rat serum IGFBPs and therefore, could be utilised to detect changes in the profile after dietary manipulations
Subject(s)
Animals , Rats , Insulin , Insulin-Like Growth Factor Binding Proteins/analysis , Methods , Plasma , Rats , SwineABSTRACT
Se presentan 8 pacientes con diagnostico de esporotricosis, comprobado por el aislamiento de Sporothrix schenckii en cultivo, quienes habian recicido tratamiento previo con yoduro de potasio si presentar mejoria o experimentar efectos secundarios que obligaran a la supresion del tratamiento. Tales pacientes fueron tratados con el nuevo derivado imidazolico, itraconazol. Todas las lesiones desaparecieron y los cultivos se negativizaron en un periodo variable de 60 - 180 dias. No se presentaron efectos secundarios, ni alteracion de las pruebas de laboratorio. Por todo lo anterior, el itraconazol se convierte en una alternativa para el tratamiento de la esporotricosis