Antihperglycaemic and Antihyperlipidaemic Activities of Aqueous Ethanol Root Extract of Pseudocedrela Kotschyi on Alloxan-Induced Diabetic Rats.

Aim: To evaluate the antihyperglycaemic and antihyperlipidaemic properties of Pseudocedrela kotschyi in alloxan induced diabetic rats. Materials and Methods: Fasting blood glucose (FBG) was conducted by first inducing diabetes through intraperitoneal administration of alloxan monohydrate (150 mg/kg body weight) (bwt). The diabetic rats, 5 per group received graded extract doses (100, 250 and 500 mg/kg) or glibenclamide (10 mg/kg) or 0.5mL acacia solution (2 %w/v) for 15 days. Blood was collected on days 0, 3, 5, 7, 9, 11, 13, 15 for glucose estimation. In postprandial test, three extract groups (100, 250 and 500 mg/kg) and the control were arranged, each comprised of 5 rats. Each animal was administered orally with glucose at a dose of 2g/kg bwt followed by extract administration 30min later. Blood glucose was monitored at 30, 60 and 120 min intervals. In hypoglycaemic study, the extract was administered at doses of 100, 250 and 500 mg/kg bwt. Lipid profile was analyzed by modified enzymatic procedure and glycated haemoglobin (HbA1C) by standard protocol. Results: The diabetic rats treated with the extract/glibenclamide showed weight gain. They also experienced dose (250 and 500 mg/kg bwt) dependent decrease in glycaemia with maximum decrease of 259.1±3.0 (24.9%) and 266.1±2.9 (25.3%) respectively while glibenclamide, 227.0±3.8 (36.0%). The postprandial test showed that the extract induced lower blood glucose level after 60 min. The extract also showed to have good hypoglycaemic activity at doses of 250 and 500 mg/kg bwt respectively. The pancreatic tissue analysis from the rats treated with the root extract indicated substantial beta cells survivor. An appreciable decrease in HbA1C level was found in the extract and glibenclamide treated compared to the negative control. In lipid profile study, Pseudocedrela kotschyi extract was observed to have ameliorated dyslipidaemia. Conclusion: The extract showed efficacy in attenuating hyperglycaemia, inducing hypoglycaemia and ameliorating dyslipidaemia.

Modulating Roles of Ethanolic Roots Extract of Crossopteryx febrifuga on Blood Glucose, Lipid Profile, Glycosylated Haemoglobin and Cytoarchitectural Changes on Pancreatic Beta Cells in Alloxan-Induced Diabetic Rats.

Aim: The study investigated the modulating roles of ethanolic roots extract of Crossopteryx febrifuga (CF) for its antihyperglycemic, antihyperlipidemic, glycosylated hemoglobin effects and cytoarchitectural changes on pancreatic beta cells in alloxaninduced diabetic rats Study Design: Experimental diabetes using animal models. Methodology: Twenty- Five (25) male albino rats were randomly divided into five (5) experimental groups: control, diabetic, standard drug (glibenclamide 10 mg/kg body wt) and C. febrifuga (375 and 500 mg/kg bwt) treated diabetic groups The animals in four out of five groups were fasted for 18 h and were made diabetic by injecting with a single dose of alloxan (ALX) 150 mg/kg, Diabetic rats 5 per group received graded doses (375 and 500 mg/kg bwt) of the extracts and glibenclamide 10 mgkg-1 for 15days. Blood was collected on days 0, 5, 10 and 15 for glucose estimation. Lipid profile was measured using DiaSys Kits from Germany which utilized the colorimetric method. Insulin Assay was measured using Monobind Insulin Microplate Elisa test while HbA1C was analyzed by Biosystem Kits (Barcelona Kits, Spain) using chromatographic method. Twenty (20) male albino rats were randomly distributed to four groups; I, II, III and IV with each consisting of five animals received 20% (w/v) glucoseorally at a dose of 0.5ml /100 g bwt. After 30 min, the animals received extracts as follows: Group I, C. febrifuga (500 mg/kg bwt); Group II, C. febrifuga (250 mg/kg bwt); Group III, C. febrifuga (100 mg/kg bwt); Group IV, 0.5 ml (2% w/v) acacia solution and served as control. Blood glucose levels were then monitored at 30, 60, and 120 min. intervals and reported as the average glucose level of each group. Results: A significant reduction in postprandial sugar level was observed after 60min in all treatments. Diabetic rats without treatment showed significant increases (p<0.05) in the levels of blood glucose, triglycerides, total cholesterol, low density lipoprotein LDL-cholesterol while the high density lipoprotein HDL-cholesterol level were significantly decreased (p<0.05) compared to normal rats. In addition, the diabetic rats treated with the CF and glibenclamide showed significant decrease (p<0.05) in blood glucose, TG and LDLcholesterol levels and a significant decrease (p<0.05) in HDL-cholesterol level compared to diabetic untreated rats. There were significant reductions (p0.05) in low density lipoprotein (LDL)-cholesterol levels and significant increase (p0.05) in the treated diabetic group compared to the negative control. Apart from these, cytoarchitectural changes also revealed the protective nature of the ethanolic roots extract of Crossopteryx febrifuga against alloxan induced necrotic damage of pancreatic tissues. Conclusion: The ethanolic roots extract of Crossopteryx febrifuga modulated hyperglycemic by potentiating insulin release from the beta cells of pancreas and ameliorated dyslipidaemia.

Testiculo Protective Effects of Ethanolic Roots Extract of Pseudocedrela kotschyi on Alloxan Induced Testicular Damage in Diabetic Rats.

Aims: The study was designed to investigate the testiculo protective effects of ethanolic roots extract of Pseudocedrela kotschyi on alloxan-induced testicular damage in diabetic rats. Study Design: Experimental diabetes using animal models. Place and Duration of Study: Department of Anatomy, College of Medicine, Lagos State University, Ikeja, Lagos, Nigeria, between January, 2013 and May, 2013. Methodology: Twenty male rats were divided into four groups: Group I consisted of nondiabetic rats that received only the vehicle; group II-IV was injected with a single dose of alloxan (ALX) of 150 mg kg-1 intraperitoneally; groups III and IV were given ethanolic roots extract of Pseudocedrela kotschyi orally, 3 days after the ALX administration, at daily doses of 250 and 500mg kg-1 respectively for a period of 30 days. After 4 weeks of treatments, all the rats were sacrificed. Results: Administration of 150 mg kg-1 of alloxan to male rats induced diabetes and significantly reduced the body and testicular weights, testosterone levels, sperm count and motility, significantly increased the glucose level and decreased the levels of antioxidant enzymatic and non-enzymatic markers such as glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and while the level of malondialdehyde (MDA) was significantly increased. By contrast, rats given the ethanolic roots extract of Pseudocedrela kotschyi had significantly increase (p<0.05) in body weight gain, whereas the glucose levels significantly improved (p<0.05) in treated diabetic male rats. In addition, this extract improved the reproductive system of the diabetic male rats by significantly increasing the testis and epididymis weights, testosterone levels, sperm count and motility, reduced testicular GSH, CAT, SOD, GPx and significantly decreasing MDA.The extract had no deleterious effects and testicular cytoprotection damaged by ALX. Conclusion: We concluded that the treatment with the ethanolic roots extract of Pseudocedrela kotschyi could reverse the adverse effects of ALX-diabetes on reproductive system of male rats which exhibits antihyperglycemic and fertility activities.