ABSTRACT
Background : The emergence of strains of S.enterica with multiple drug resistance (MDR) is of great concern worldwide.The extracts of flowers of Thonningia sanguinea are used in traditional medicine in Ivory Coast to treat diarrhoeal diseases including salmonellosis. Previous studies had shown inhibition of the MDR strain Salmonella Enteritidis lysotype 6. Objectives: The present study focused to investigate the effect of the extract of the flowers of Thonningia sanguinea on some clinical MDR strains of Salmonella namely S.Tyhpi; S. Typhimurium; S. Hadar and a sensitive strain (S.Enteritidis). Methods : The antimicrobial parameters were determined by double dilution with agar slant method. This method led us to determine MIC; IC 50 and MBC. Results: The MDR strain of S.Typhimurium presented the highest MIC (2.5 mg/ml) whereas the other two MDR strains (S.Hadar; S.Typhi) and the sensitive one (S.Enteritidis) had the same MIC (1.25 mg/ml). The four strains presented the same MBC (2.5 mg/ml). The MDR strain of S.Typhi is the most susceptible strain to the aqueous extract of the flowers of Thonningia sanguinea according to The IC 50 values. Conclusions: The aqueous extract of Thonningia sanguinea can provide an alternative therapy for the treatment of salmonellosis; mainly for typhoid fever caused by MDR strains of S Typhi. The extract also inhibits S.Hadar a MDR emerging strain in Ivory Coast
Subject(s)
Anti-Bacterial Agents , Balanophoraceae , Plant Extracts , Salmonella enterica , Sprains and StrainsABSTRACT
Nous avons teste l'action des extraits (extrait total aqueux; extrait ethanolique 70; residu insoluble dans l'ethanol 70; extrait ethanolique 100et extrait butanolique) de Mitracarpus scaber (Rubiaceae) sur la croissance in vitro des streptocoques ? hemolytiques en milieu liquide et sur milieu solide de Milieu-Hinton. Cette plante codifiee MISCA dans notre etude; a toujours ete utilisee dans la pharmacopee ivoirienne contre les infections cutanees. Les resultats de notre etude montrent que les souches des treptocoques ? hemolytiques sont sensibles a tous les extraits testes. Cependant; seul l'extrait butanolique a une meilleure activite bactericide sur la croissance in vitro de ces souches. Les parametres antibacteriens obtenus sont les suivants : CMI = 3;13 mg/ml; CMB = 0;39 mg/ml et CI50 = 0;02 mg/ml