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1.
Rev. argent. microbiol ; 32(1): 39-43, ene.-mar. 2000.
Article in Spanish | LILACS | ID: lil-332539

ABSTRACT

An indirect enzyme linked immunosorbent assay was developed. Infected and non infected allantoic fluids precipitated with polyetilenglycol 6000 were used as antigen and control antigen, respectively. Serum samples were diluted 1/20 and a commercial horse radish peroxidase-labelled rabbit anti-equine IgG was used as second antibody. The reaction was developed using azino-diethylbenzotyazol-sulfonate (ABTS). Cut-off was determined by ratio sample (Rs). The hemagglutination inhibition test was used as a reference test for the 391 samples analyzed. Of these, 301 sera were positive by hemagglutination inhibition test and indirect ELISA, 75 were negative by both techniques, and 15 were positive by indirect ELISA and negative by hemagglutination inhibition test. Using hemagglutination inhibition test as standard, the indirect ELISA showed a relative specificity and sensitivity of 83.3 and 100, respectively. This indirect ELISA is useful as screening test.


Subject(s)
Humans , Animals , Rabbits , Enzyme-Linked Immunosorbent Assay , Orthomyxoviridae Infections/diagnosis , Influenza, Human , Influenza A virus/isolation & purification , Hemagglutination Inhibition Tests , Sensitivity and Specificity
2.
Arq. bras. med. vet. zootec ; 51(4): 299-305, ago. 1999. ilus, tab
Article in Portuguese | LILACS | ID: lil-260991

ABSTRACT

Um sistema de western blotting (WB) foi desenvolvido para detecçäo de anticorpos contra o vírus da leucose em soros bovinos. Foram utilizadas amostras de soros de 233 animais naturalmente infectados e soros de vacas experimentalmente infectadas. O teste de imunodifusäo em ágar (AGID) foi usado para comparaçäo dos resultados. Graus diferentes de reatividade foram observados em soros positivos ao AGID, quando testados em WB frente a um ou aos dois antígenos mais importantes (gp51 e p24). Outras proteínas (gp30, p15, p12 e p10) näo foram detectadas por nenhum soro positivo ao AGID, sendo que três bandas correspondentes à proteína p24 foram observadas ocasionalmente. Em soros obtidos por inoculaçäo experimental, anticorpos contra a proteína p24 foram detectados entre a segunda e a quarta semanas após a inoculaçäo e, em alguns animais, detectaram-se anticorpos anti-gp51 mais tardiamente. O estudo de soros de campo com AGID e WB mostrou concordância de 90,9 por cento sendo que apenas 1,7 por cento dos soros negativos pelo AGID foram positivos ao WB e 7,2 por cento dos resultados näo conclusivos por AGID foram definidos por WB (4,2 por cento como positivos e 3 por cento como negativos)


Subject(s)
Animals , Male , Female , Blotting, Western , Diagnosis , Enzootic Bovine Leukosis , Cattle
3.
Braz. j. med. biol. res ; 31(6): 771-4, jun. 1998. ilus
Article in English | LILACS | ID: lil-210964

ABSTRACT

The genomes of 10 equine herpesvirus 1 (EHV-1) strains isolated in Argentina from 1979 to 1991, and a Japanese HH1 reference strain were compared by restriction endonuclease analysis. Two restriction enzymes, Bam HI and Bgl II, were used and analysis of the electropherotypes did not show significant differences among isolates obtained from horses with different clinical signs. This suggests that the EHV-1 isolates studied, which circulated in Argentina for more than 10 years, belong to a single genotype


Subject(s)
Genetic Variation , Genome , Herpesvirus 1, Equid/genetics , Argentina , Deoxyribonuclease BamHI , Deoxyribonucleases, Type II Site-Specific , Electrophoresis , Herpesvirus 1, Equid/isolation & purification
4.
Rev. argent. microbiol ; 20(3): 137-40, 1988. tab
Article in Spanish | LILACS | ID: lil-78143

ABSTRACT

Se comparó la morfología de las placas de lisis producidas pro tres cepas de virus Herpes equino tipo 1: SP1, aislada en nuestro laboratorio, y dos cepas de referencia, Kentucky B adaptada a cultivos celulares (Ky Bcc) y Kentucky B atenuada por pasajes en hamster (Ky Ba). Se observó que la cepa Ky Bcc formó placas pequeñas de tamaño variable, de aproximadamente 1 mm de diámetro y de bordes irregulares. La cepa Ky Ba formó placas de tamaño uniforme de aproximadamente 2-3 mm y bordes lisos. La morfología de las placas formadas por la cepa SP1 fue coincidente con Ky Bcc. Se concluye que la cepa SP1 es una cepa salvaje de origen abortigénico y semejante a la cepa Ky Bcc en su propiedad de formar placas líticas


Subject(s)
Animals , Herpesviridae/growth & development , Herpesvirus 1, Equid/growth & development , Viral Plaque Assay , Herpesvirus 1, Equid/classification
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