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1.
Mem. Inst. Oswaldo Cruz ; 99(1): 53-55, Feb. 2004. graf
Article in English | LILACS | ID: lil-356443

ABSTRACT

The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r² = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.


Subject(s)
Humans , Male , Female , Adult , Interferon-gamma , Mycobacterium tuberculosis , T-Lymphocytes , Tuberculosis , Cell Culture Techniques , Enzyme-Linked Immunosorbent Assay , Interferon-gamma , Tuberculin Test , Tuberculosis
2.
Mem. Inst. Oswaldo Cruz ; 97(8): 1097-1099, Dec. 15, 2002. tab, graf
Article in English | LILACS | ID: lil-326339

ABSTRACT

The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease


Subject(s)
Antigens, Bacterial , Antigens, CD , Antigens, Differentiation, T-Lymphocyte , CD4-Positive T-Lymphocytes , CD8-Positive T-Lymphocytes , Lymphocyte Activation/immunology , Mycobacterium tuberculosis , Receptors, Interleukin-2 , Acyltransferases , Bacterial Proteins , Ferritins , Flow Cytometry , Tuberculin
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