ABSTRACT
Current retrospective analysis describes the laboratorial profile of patients hypothetically diagnosed with dengue at a First-Aid Health Unit in Recife, Pernambuco State, Brazil. Results of hemograms were assessed by counting platelets and transaminase dosages, in the first half of 2012. Further, 394 patients (252 females or 64% and 142 males or 34%) were listed, mostly during March and April. Hemograms with platelet counting was undertaken with 210 patients (53.3%) and hemogram plus transaminases dosages with 184 patients (46.7%). Thrombocytopenia, neutropenia and atypical lymphocytes occurred in both genders. Hematocrits were highest in males and transaminases were more altered in females. Patients attended at the health unit with clinical symptoms of classical dengue had a laboratory profile of non-specific exams which is a feature of infection by the dengue virus. Diagnosis could not be confirmed and the need of a fast test in the health unit services was mandatory. The above avoids dengue cases not being notified and treated or overestimated.
Estudo retrospectivo que objetivou descrever o perfil laboratorial dos usuários com hipótese diagnóstica de dengue em um Serviço de Pronto Atendimento (SPA) do Recife (PE). Foram avaliados resultados do hemograma com contagem de plaquetas e dosagem de transaminases, no primeiro semestre de 2012. Registraram-se 394 usuários, sendo 252 do sexo feminino (64%) e 142 do sexo masculino (36%). Hemograma com contagem de plaquetas foi realizado em 210 usuários (53,3%) e hemograma junto com dosagem de transaminases em 184 (46,7%). Trombocitopenia, neutropenia e linfócitos atípicos ocorreram em ambos os sexos. O hematócrito mostrou maior elevação em pacientes do sexo masculino. As transaminases estavam mais alteradas nas mulheres. Os usuários atendidos no SPA com sintomas clínicos de dengue clássica apresentaram perfil laboratorial de exames inespecíficos, característico de infecção pelo vírus da dengue, porém, o diagnóstico não pôde ser confirmado. Isto mostra a necessidade de implantação do teste rápido para dengue nos serviços de pronto atendimento, o que irá evitar que casos de dengue não sejam notificados e tratados, assim como outros sejam superestimados.
Subject(s)
Humans , Male , Female , Thrombocytopenia , Blood Cell Count , Dengue , Transaminases , NeutropeniaABSTRACT
The selection of new microorganisms able to produce antimicrobial compounds is hoped for to reduce their production costs and the side effects caused by synthetic drugs. Clavulanic acid is a β-lactam antibiotic produced by submerged culture, which is widely used in medicine as a powerful inhibitor of β-lactamases, enzymes produced by bacteria resistant to antibiotics such penicillin and cephalosporin. The purpose of this work was to select the best clavulanic acid producer among strains of Streptomyces belonging to the Microorganism Collection of the Department of Antibiotics of the Federal University of Pernambuco (DAUFPE). Initially, the strains were studied for their capacity to inhibit the action of β-lactamases produced by Klebsiella aerogenes ATCC 15380. From these results, five strains were selected to investigate the batch kinetics of growth and clavulanic acid production in submerged culture carried out in flasks. The results were compared with the ones obtained by Streptomyces clavuligerus ATCC 27064 selected as a control strain. The best clavulanic acid producer was Streptomyces DAUFPE 3060, molecularly identified as Streptomyces variabilis, which increased the clavulanic acid production by 28% compared to the control strain. This work contributes to the enlargement of knowledge on new Streptomyces wild strains able to produce clavulanic acid by submerged culture.