ABSTRACT
Objective: To evaluate the antipiroplasmic activities of methanolic extract of Olea europaea (MOE) and acetonic extract of Acacia laeta (AAL) against Babesia and Theileria parasites in vitro and evaluate the chemotherapeutic effects of these extracts against Babesia (B.) microti in vivo. Methods: Fluorescence assay using SYBR Green 1 nucleic acid stain was used to detect inhibitory effects of the two extracts as well as the combination effects of the two extracts with diminazene aceturate and atovaquone on four Babesia species and Theileria equi in vitro while for in vivo experiments, 8-weekold female BALB/c mice were injected intraperitoneally with 1 × 10
ABSTRACT
Silver nanoparticles are being used increasingly for biomedical purposes because of their broad antimicrobial potential. Their effects on cellular systems, however, have been addressed in only a few studies. We evaluated the effects of these nanoparticles on the antioxidant status of groups of five male Wistar rats. Six groups of rats were given 100, 1000 or 5000 mg/kg daily through an oral cannula for 7 or 14 days, one group received 5000 mg/kg for 21 days, and a control group received distilled water. The animals were sacrificed 24 h after the end of treatment, and serum and tissue homogenates were prepared. Silver nanoparticles significantly [p < 0.05] increased the concentrations of malondialdehyde and superoxide dismutase but decreased the levels of reduced glutathione, glutathione S-transferase and catalase. These results indicate that silver nanoparticles may cause lipid peroxidation and alter antioxidant status in a manner that may cause oxidative stress