ABSTRACT
PURPOSE: The purpose of this study was to evaluate the clinical efficacy of reverse transcription- polymerase chain reaction (RT-PCR) in detecting rotaviral gastroenteritis in children comparing with that of commercial immunoassays. METHODS: Stools from 79 children admitted Korea University Hospital due to diarrhea were collected from December 1999 to February 2000. Immunoassays were done using commercial rotavirus Latex kit and Rotatec (ELISA) kit. RT-PCR was performed to amplify group A rotavirus, most commonly pathogenic to human, using VP4- and VP7-specific primers. The detection rates of immunoassays and RT-PCR were compared. RESULTS: ELISA assay was superior to LA assay and moderately concordant with RT-PCR in detecting rotaviral gastroenteritis. CONCLUSION: Although RT-PCR is known very sensitive, it does not have significant advantage over immunoassay in detecting rotaviral gastroenteritis.
Subject(s)
Child , Humans , Diagnosis , Diarrhea , Enzyme-Linked Immunosorbent Assay , Gastroenteritis , Immunoassay , Korea , Latex , Polymerase Chain Reaction , RotavirusABSTRACT
A 59-year-old female patient with rheumatoid arthritis showed hypokalemic metabolic alkalosis, normotensive hyperreninemic hyperaldosteronism and high urinary prostaglandin level. She was thought to have Bartter's syndrome. But, her kidney biopsy specimen showed chronic interstitial nephritis. She have used acetaminophen containing analgesics for recent three years. So we thought her disease was caused by drug. But, in this case, clinical manifestations are correspond with Bartter's syndrome and we have witnessed a successful respond to kalium replacement, angiotensin converting enzyme inhibitor, prostaglandin inhibitor and spironolactone administration.
Subject(s)
Female , Humans , Middle Aged , Acetaminophen , Alkalosis , Analgesics , Arthritis, Rheumatoid , Bartter Syndrome , Biopsy , Glycogen Storage Disease Type VI , Hyperaldosteronism , Kidney , Nephritis, Interstitial , Peptidyl-Dipeptidase A , Prostaglandin Antagonists , SpironolactoneABSTRACT
BACKGROUND: Different distributions of cytokeratins (CKs) have been found in various layers of the epidermis and in the structure of cutaneous appendages. Benign tumors of the epidermis or cutaneous appendages usually retain the characteristic distribution of CKs of their origin. OBJECTIVE AND METHOD: To elucidate the nature and differentiation of various eccrine gland tumors, we performed immunohistochemical staining using a panel of monoclonal antibodies against CKs in a normal eccrire gland, syringoma, eccrine hidrocystoma, eccrine poroma and clear cell hidradenoma. The tissue distribution of various keratins in the normal eccrine gland was compared with that in varioas eccrine gland tumors. RESULTS: With regard to CKs expression, the results indicated that syringoma represents a tumor differentiating toward both the uppermost part of the dermal duct and the lower intraepidermal duct, and eccrine hidrocystoma represents a tumor differentiating toward the luminal cell of the dermal duct of the eccrine gland. Eccrine poroma cells seemed to be most closed related to the uppermost dermal duct of eccrine gland. We also found that analyse distribution of CKs was insufficient to elucidate the histogenesis of clear cell hidradenoma. CONCLUSION: Analysing CKs is helpful in the analysis of epidermal neoplasms with differentiation toward the eccrine gland.
Subject(s)
Acrospiroma , Antibodies, Monoclonal , Eccrine Glands , Epidermis , Hidrocystoma , Keratins , Peptides , Phenobarbital , Poroma , Syringoma , Tissue DistributionABSTRACT
BACKGROUND: Different distributions of cytokeratins (CKs) have been found in various layers of the epidermis and in the structure of cutaneous appendages. Benign tumors of the epidermis or cutaneous appendages usually retain the characteristic distribution of CKs of their origin. OBJECTIVE AND METHOD: To elucidate the nature and differentiation of various eccrine gland tumors, we performed immunohistochemical staining using a panel of monoclonal antibodies against CKs in a normal eccrire gland, syringoma, eccrine hidrocystoma, eccrine poroma and clear cell hidradenoma. The tissue distribution of various keratins in the normal eccrine gland was compared with that in varioas eccrine gland tumors. RESULTS: With regard to CKs expression, the results indicated that syringoma represents a tumor differentiating toward both the uppermost part of the dermal duct and the lower intraepidermal duct, and eccrine hidrocystoma represents a tumor differentiating toward the luminal cell of the dermal duct of the eccrine gland. Eccrine poroma cells seemed to be most closed related to the uppermost dermal duct of eccrine gland. We also found that analyse distribution of CKs was insufficient to elucidate the histogenesis of clear cell hidradenoma. CONCLUSION: Analysing CKs is helpful in the analysis of epidermal neoplasms with differentiation toward the eccrine gland.
Subject(s)
Acrospiroma , Antibodies, Monoclonal , Eccrine Glands , Epidermis , Hidrocystoma , Keratins , Peptides , Phenobarbital , Poroma , Syringoma , Tissue DistributionABSTRACT
Condyloma acuminatum occurs in perianal and genital area by infection of human papillomavirus (HPV) and appeais as fairly soft verrucous papules or a cauliflower-like mass. So far 60 HPV types have been identified. Among them, condyloma acuminatum is predomi-nantly associated with HPV 16, 18 and rarely with 16, 18. HPV 16, 18 is regarded as a high-risk HPV infection because of the association of HPV 16, 18 with Bowens disease, cervical cancer, and anogenital cancer. In additioin, a significant appearance of cervical anaplastic lesions is preceded by condyloma acuminatum and a high prevalence of cervical dysplasia has been found in female consorts of men with geriital warts. Malignant transformation has been observed in rare incidences of condyloma acumina a on the external genitalia. We present a case of condylc ma acuminatum showing malignant transforrnation. The patient also has uterine cervical carcinonia. We detected HPV 16 in the lesion using polymerase chain reaction.
Subject(s)
Female , Humans , Male , Bowen's Disease , Genitalia , Human papillomavirus 16 , Incidence , Polymerase Chain Reaction , Prevalence , Uterine Cervical Neoplasms , WartsABSTRACT
Artichia with papular lesions is a rare congenit,al disease characteriezed by the association of alopecia and papular lesions. We report a case of atrichia with papular lesions in 12-year-old. Alopecia of the scalp and eye brows were present at birth. Nurnerous papular lesions are presentation the trunk, buttock and inguinal area. The histopathologic iinding of a papular lesion showed ker till-filled cyst, and eruptive vellous hair cyst. The patient showed reticulat.ed hyperpigmentation on the hands and feet in addition to the characteristic feature of atriclia with papular lesions.
Subject(s)
Child , Humans , Alopecia , Buttocks , Foot , Hair , Hand , Hyperpigmentation , Parturition , ScalpABSTRACT
Reticulate acropigmentation of Kitamura (RAPK) is an autosomal dominant dermatosis comprising of reticulate slightly clepessed pigmentation of the extensor surfaces of the hands and feet with palmar pits. It has been suggested that this may be the same disease as Dawling Degos disease (DDD), an autosomal dorninant condition which is characterized by a reticulate pigmentation of flexures, comedo-like lesicns and pitted scars. We present a case of RAPK in a 49-year-old female who had reticulate, brownish, slightly depressed pigmentation on the extremities with t,he involvement of flexures, the predilection sites of DDD.