ABSTRACT
In spite of the availability of effective antimicrobial therapy, Otitis media with effusion [OME] is still an important infection leading to serious health problems in both children and adults. [Streptococcus pneumonia] S. pneumoniae is one of the most frequently isolated bacteria from middle ear effusion [MEE] samples of OME patients. Since it is fastidious bacteria, various problems may arise in the rapid diagnosis of OME settings. Knowing which bacterium is involved is essential for the prognosis and treatment of otitis media with effusion, as the high frequency of its presentation may be linked to the etiology and/or course of the process in many patients
Objective: the aim of this study was to evaluate the use of nested polymerase chain reaction [PCR] assay for S.pneumonia as a diagnostic tool in patient with OME, and to detect the relation of the organism to other factors contributed to pathogenesis of OME
Subjects and Methods: middle ear fluid samples were aspirated from 34 patients presented with OME. Each sample was subjected to cultivation on selective media, and nested PCR test using specific primers directed to selected pneumolysin gene fragment of S.pneumonia
Results: S.pneumonia was recovered by culture in 4/34 [11.87%] of middle ear fluid samples; 3/4 [75%] from children and ¼ [25%] from Adults. Nested PCR detected S.pneumonia in 11/134 [32.4%] of the samples; 8/11 [72. 7%] were children and 3/11 [27.3%] were adults. All culture positive samples were PCR positive, but 7 [63.6%] of the PCR positive samples were culture negative. There were strong effects of the age, pervious history of acute otitis media, sinusitis and allergic rhinitis. The recovery rate of the organism was [72.7%, 54.5 %, 36.4% and 27.3%] respectively
Conclusion S.pneumonia was detected in high rates in MEE samples. This suggests that it may play a role in the pathogenesis of otitis media with effusion. In addition, PCR was more sensitive compared to culture for detection of S. pneumoniae in MEE samples
ABSTRACT
Fungi have been increasingly recognized as important pathogens in sinusitis. Fungal infection, mainly by moulds, can impose a severe acute and chronic sinusitis in the immunocompromised host as chronic myeloid leukemia. Polymerase Chain Reaction [PCR] is an important tool in diagnosis of fungi even in small amount in the secretions. Is to detect the causative pathogenic fungi in chronic maxillary sinusitis in cases of chronic myeloid leukemia patients by using PCR technique. 20 patients of chronic myeloid leukemia suffing from maxillary sinusitis and 20 pathients with chronic maxillarry sinusitis not suffering from any immunocompromised disease were used as control group, both groups were subjected to intranasal antrostomy then, antral lavage were subjected to fungal culture and PCR techniques. PCR detect 80% positive fungi in test group but there was negative in control group, Aspergillus spp., A. fumigatus and Penicillium spp. and Candida were present in positive cases, 20% of cases were detected by simple fungal culture. There is significant difference between PCR and fungal culture [P<0.05]. PCR technique is sensitive tool in diagnosis of fugal infection in chronic maxillary sinusitis in patients with chronic myeloid leukemia; PCR is superior to ordinary fungal culture and can detect funji even in small amount of fungal spores, Aspergillus spp., A. fumigatus and Penicillium spp. and Candida albicans were present in cases of chronic myeloid leukemia with sinusitis
Subject(s)
Humans , Male , Female , Maxillary Sinusitis , Fungi , Polymerase Chain ReactionABSTRACT
The treatment and method of rehabilitation of laryngeal cancer depends on the clinical staging and site of the tumour .The early detection of laryngeal cancer and less tissue loss leads to good results of rehabilitation. Current methods of vocal rehabilitation after total laryngectomy include development of esophageal speech, use of electro-larynx and Trach-eoesophageal fistula with prosthesis. Also the early detection of laryngeal cancer will make a minor tissue loss after procedures, and so limit the problems of rehabilitation [speech and swallowing]. The aim of this study is to compare the voice results of patients who underwent: total laryngectomy with provox prosthesis and conservative laryngectomy. This study included 2 groups; 19 patients who underwent laryngectomy for control of laryngeal carcinoma subdivided into 2 groups according to surgical procedures 9 patients with total laryngectomy with provox processes and 10 patients with conservative laryngectomy and 6 normal as control group. After voice analysis for the two test groups and comparison to the control group, there was significant difference between conservative group and provox group as regards intelligibility score and number of words per minute and also Shimmer and noise to harmonic tests of this study showed that conservative laryngectomy group is relatively better than provox group in many parameters of analysis; this may be due less tissue loss in conservative group