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Aims@#The methylotrophic yeast Pichia pastoris is widely used to express foreign proteins fused to secretion signals. As the effect of the expression host on the final protein product is unclear, we compared the properties of an endoglucanase (eglB of Aspergillus niger) expressed in two different P. pastoris strains. @*Methodology and results@#Full-length cDNA encoding endoglucanase of A. niger strain ATCC10574 was isolated and expressed in P. pastoris X33 (the methanol utilisation plus phenotype, Mut+) and P. pastoris GS115 (slow methanol utilisation, MutS). EglB-GS115 showed the highest activity and stability at 60 °C while EglB-X33 was most active at 50 °C. EglB-X33 was active towards other substrates such as arabinogalactan, guar gum and locust bean gum besides its specific substrate, carboxymethyl cellulose (CMC). However, EglB-GS115 was only active on CMC. The affinity of EglB-X33 towards CMC (Km = 7.5 mg/mL and specific activity 658 U/mg) was higher than that of EglB-GS115 (Km = 11.57 mg/mL, specific activity 144 U/mg). @*Conclusion, significance and impact of study@#Although eglB was cloned in the same expression vector (pPICZαC), two different characteristics of enzymes were recovered from the supernatant of the different hosts. Thus, expression of recombinant enzyme in different P. pastoris strains greatly affects the physical structure and biochemical properties of the enzyme.
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Aim: There is still a need for new, selective COX-2 inhibitors with an improved safety profile, therefore, a series of diclofenac analogues were designed and different physicochemical properties were calculated such as log P, hydrogen donor, hydrogenacceptor, molecular weight and pKa etc and compared with diclofenac and study was aimed to design and calculate different physicochemical properties and attempt to introduce diclofenac derivatives with improved anti-inflammatory profile along with docking focusingon CO X-2. Materials and Method: Carrageenan-induced paw edema to evaluate the antiinflammatory activity of the conjugates 4 groups (n = 6) of Wistar rats (150–200 g) were examined. A first group of rats was used as a control without using the drug, group II received Diclofenac 20 mg kg–1, received PEG600-Diclo conjugate and PEG4000-Diclo conjugate (52.60 mg kg−1 and 214 mg kg−1 respectively), where the dose was molecularly equivalent to the diclofenac. Results: Result showed a significant (p<0.05) dose dependent increase in reaction time in mice in the method at the doses of 150 and 200 mg/kg body weight. Also docking studies specifically on COX-2 exhibited promising anti-inflammatory effect as demonstrated by statistically significant (p<0.05) inhibition of paw volume at the dose of 150 mg/kg body weight and the dose of 500 mg/kg body weight at the three hours of study. Conclusion: In this study molecular docking results, along with biological assay data, show that all compounds have a potential anti-inflammatory activity comparable to diclofenac.
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Introduction:The Republic of Djibouti is an African country that exhibits one of the highest incidence rate of tuberculosis in the world. The aim of this study was to evaluate the prevalence of multidrug resistant tuberculosis among new cases. Methodology: We studied retrospectively every tuberculosis case diagnosed over a 12month period in patients hospitalized at the French military Hospital of Bouffard. During this period; 1;274 samples from 675 patients were tested. Results: We isolated 266 mycobacteria corresponding to 180 cases of tuberculosis. Thirty three were fully susceptible and 57 met the tuberculosis criteria; with 46 primary resistance. No extensively drug resistant tuberculosis was found. Conclusion: Our results highlight a major concern about the situation in this part of the world
Subject(s)
Mycobacterium tuberculosis , Prevalence , TuberculosisABSTRACT
Staphylococcus aureus [S. aureus] is one of the most common causes of both community and hospital acquired bacterial infection. There is strong correlation between S aureus nasal carriage and disease progress. Nasal carriage is high among health care workers. Inappropriate usage of antibiotic may lead to emergence of resistant strains which has serious consequences. The objective of this study is to reveal the frequency of S aureus nasal carriage and its drug resistance among surgical personnel in National Ribat Teaching Hospital Khartoum Sudan. This is a hospital-based case study. Nasal smears were taken from medical workers in the surgical department and operational theater at National Ribat Teaching Hospital in Khartoum State, Sudan. Samples were processed, cultured, then susceptibility tests were performed using Bauer-Kirby disc diffusion methods following recommendations of National Committee for Laboratory Standards [NCCLS]. Results were analyzed and discussed. Sixty three samples were taken. Thirty were males. Growth was achieved in only eight [12.6%]. Majority showed resistance to penicillin. However, alls strain were sensitive to amoxicillin/calvunalic acid, vancomycin and oxacillin. This study gives an early alarm on the problems related to S. aureus colonization rate and its drug resistance. Nevertheless, the small number of our study group is a bit fall