ABSTRACT
Objective To study the expressions of DD3 mRNA and PSA mRNA in the prostate tissues and its diagnostic value in prostate cancer (PCa). Methods DD3 mRNA and PSA mRNA were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction ( FQ-RT-PCR) based on Taqman technique in the tissues of 21 cases of PCa and 39 cases of BPH. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of DD3 mRNA, PSA mRNA and DD3 mRNA/PSA mRNA. Results The expressions of DD3 mRNA and PSA mRNA, and DD3 mRNA/ PSA mRNA were significantly higher in PCa tissues than those in BPH tissues ( P 0.05 for all). The AUC-ROC of DD3 mRNA,PSA mRNA and DD3 mRNA/PSA mRNA were 0. 937 (95% CI,0. 879 -0. 995) , 0.755(95% CI,0.629 -0.880) and 0.839 (95%CI,0.738 -0.940),respectively. The sensitivity for DD3 mRNA,PSA mRNA and DD3 mRNA/PSA mRNA was 90. 5% ,81. 0% and 81. 0% , respectively, and the specificity was 85.0% ,62.0% and 66.7% at cutoff value of 1.4?105 copies/mg tissue,3.0?107 copies/ mg tissue and 5. 0?10-3,respectively. The sensitivity and specificity of simultaneous detection for DD3 mRNA and PSA mRNA were 100% and 85.0%. Conclusions Both DD3 mRNA and PSA mRNA expressions were significantly higher in PCa tissues than those in BPH tissues; and the quantitative detection of DD3 mRNA is more helpful for the diagnosis. The simultaneous detection of DD3 mRNA and PSA mRNA can improve the sensitivity in the diagnosis of PCa.
ABSTRACT
Objective To compare strong and weak points of two different approaches of endoscopic thyroidectomy and to discuss the complication prevention of endoscopic thyroidectomy.Methods There were 10 cases of breast areola approach endoscopic thyroid surgery(Areola Group) and 11 cases of transcervical approach video-assisted thyroid surgery(Cervical Group).The operation time,intraoperative blood loss,length of hospital stay,and complications between the two groups were compared.Results The operations were completed successfully in all the 21 cases.The operation time was significantly longer in the Areola Group(132.4?39.1 min) than in the Cervical Group(89.2?16.8 min)(t=3.347,P=0.003).The intraoperative blood loss was significantly less in the Areola Group(12.9?4.3 ml) than in the Cervical Group(18.2?5.5 ml)(t=-2.442,P=0.025).The postoperative hospital stay was significantly longer in the Areola Group(4.2?1.0 d) than in the Cervical Group(3.0?0.6 d)(t=3.373,P=0.003).Follow-up observations for 6 months in the 21 cases showed no recurrence of thyroid tumor or occurrence of hypothyroidism.Conclusions Compared with video-assisted thyroidectomy through transcervical approach,breast areola approach endoscopic thyroid surgery has longer operation time,but more satisfactory exposure,fewer complications,and better cosmetic outcomes.