Effect of Garcinia binucao crude leaf extract supplementation on lifespan of Drosophila melanogaster chronically exposed to alcohol

Background@#Consumption and abuse of alcohol remains a significant cause of concern worldwide. Furthermore, there is evidence of the association between chronic alcohol use and reduced life expectancy. @*Objectives@#To study the effects of Garcinia binucao extract (GBE) supplementation on lifespan of Drosophila melanogaster, in the presence or absence of chronic alcohol exposure.@*Methods@#D. melanogaster was mass cultured and given GBE supplementation in high (1 mg/mL) and low (200 µg/mL) sublethal doses. D. melanogaster flies were divided into groups - with and without chronic alcohol exposure, and their respective lifespans were monitored. @*Results@#In D. melanogaster without alcohol exposure, mean lifespan was highest in the control flies (38.15 days), followed by high-dose GBE (34.42 days), low-dose GBE (33.24 days), and DMSO (22.29 days). In D. melanogaster chronically exposed to alcohol, the longest mean lifespan was observed in flies treated with high-dose GBE (33.80 days), followed by low-dose GBE (33.63 days), the DMSO group (30.30), and the control group (29.65 days), but the differences were not statistically significant. Comparing groups with and without chronic alcohol exposure, the mean lifespan of the control group chronically exposed to alcohol significantly decreased by 9.51 days (p < 0.05). In GBE treatment groups, mean lifespan significantly decreased by 0.82 days in high-dose set-up (p < 0.05), and significantly increased by 0.39 days in the low-dose set-up (p < 0.05) upon chronic alcohol exposure. @*Conclusion@#Garcinia binucao extract supplementation ameliorated the observed reduction in lifespan of Drosophila melanogaster chronically exposed to alcohol.

Ethnomedicinal survey of valuable plants used by Eskaya Traditional Healers in Bohol Island, Philippines

Objectives@#A recent review on the status of Philippine ethnobotany showed the lack of ethnobotanical studies in the Visayas group of islands. The use of medicinal plants by the Eskaya, an Indigenous ethnic group in the province of Bohol in Visayas, Philippines, has not yet been documented. This study determines the ethnomedicinal knowledge of traditional healers in selected Eskaya communities in Bohol.@*Methods@#Fifteen (15) Eskaya traditional healers were interviewed using a structured questionnaire, and the valuable medicinal plant species were identified through guided field walks. Informal group discussions with the community were also conducted. Moreover, the fidelity level, informant consensus factor (ICF), and use values (UV) were calculated.@*Results@#Our results showed that the majority of the Eskaya healers belong to an older age group (38 – 71 years old). Eighty-five medicinal plants recorded belong to 44 plant families—with Family Lamiaceae having the highest number of species represented. The leaf is the most common plant part used for healing. Blumea balsamifera was an essential plant with a UV of 0.8. Cardiovascular diseases have the highest ICF values of 0.6, and the most common plants used for these diseases include Cymbopogon citratus, Zingiber officinale, and Luffa cylindrica.@*Conclusion@#Our study yielded a record of native and non-native medicinal plants used by the Eskaya healers. This initial listing is also their contribution to promoting medicinal plants that are accessible and abundant in their communities.

Regional differences in online interest in COVID-19 infection and COVID-19 vaccine in pregnancy: A Google Trends analysis / Philippine Journal of Obstetrics and Gynecology

Background@#With the advent of the COVID-19 pandemic, pregnant women may turn to online information searches regarding COVID-19 and COVID-19 vaccination. @*Objective@#We aimed to determine global online interest in COVID-19 infection and COVID-19 vaccination in pregnancy. @*Materials and Methods@#We utilized Google Trends data to determine the global search volume index (SVI) for the search terms “COVID-19 in pregnancy” and “COVID vaccine pregnancy” from January 1, 2020, to February 12, 2022, and ranked the top 15 search queries via their individual SVIs to assess the worldwide distribution of interest. Using Pearson correlation, we correlated several economic and pandemic-related country-specific data with the SVIs for the two search terms, while we utilized a one-way analysis of variance to country income groups with the SVIs using ANOVA. Significant correlations were denoted with P < 0.05.@*Results@#SVI for “COVID-19” in pregnancy showed a minimal and nonsignificant downward trend in 2022. SVI for this search term was positively correlated with the SVI for “COVID vaccine pregnancy” (P < 0.05). On the other hand, SVI for “COVID vaccine in pregnancy” showed an exponential decline from November 2020 to 2022. SVI for this search term was also negatively correlated with deaths per 100,000 cases (P < 0.05). Although this was nonsignificant, high-income countries showed higher online interest with the two search terms than low- to low-middle-income countries. There were no significant correlations among specific economic and pandemic-related country-specific data and SVI for both search terms. @*Conclusion@#We observed a constant online interest in COVID-19 in pregnancy during the time period of the study, which may reflect the watchful engagement of people in the absence of face-to-face physician consultations. Peak online interest in COVID-19 vaccine in pregnancy declined steadily, which may reflect either vaccine hesitancy or an increase in public knowledge of COVID-19 vaccines. High-income countries have apparently higher online interest in COVID-19 vaccines, which may be due to access to early mass administration to the general public. These data can be used as a reference regarding future policy-making in the setting of potentially massive public health emergency in the future.

Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines

Objective: To evaluate the cytotoxic activities of Premna odorata (P. odorata) leaves and bark, Artocarpus camansi (A. camansi) and Gliricidia sepium against selected human cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methods: The crude extracts of P. odorata, A. camansi and Gliricidia sepium were subjected to liquid-liquid partitioning by using hexane and ethyl acetate to separate compounds based on their polarity. The fractions were tested for their cytotoxic activity against human colon cancer cell line (HCT116), breast cancer cell line (MCF-7), lung adenocarcinoma cell line (A549) and Chinese hamster ovary cell line (AA8) by using MTT assay. Results: Based on the standard values of toxicity set by the study of Suffness and Pezzuto, P. odorata leaves and P. odorata bark hexane fractions and A. camansi leaves were all considered highly cytotoxic against the selected human cancer cell lines. P. odorata bark hexane extract exhibited the highest selectivity index for HCT116, MCF-7 and A549 cancer cell lines. Conclusions: The results obtained indicated that P. odorata leaves and bark and A. camansi leaves have excellent cytotoxic activity and warrant further studies to isolate novel compounds for chemotherapeutic use.