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@#BACKGROUND OF STUDY: Not only in developed countries but also in Mongolia it is vital problem to decide how to improve human life’s quality, to make clear the measurement to take, discover human body’s change, aging mechanism and predict from these diseases. The research on herders’ biological aging which is connected to the geography, nature, environment, climate, inhabitant, culture and labor’s specialty hasn’t been done yet. So it causes the basis to write this research. AIMS: Defining the nomadic herder’s biological aging MATERIAL AND METHODOLOGY: All the herders in Gobi-Altai were included in this research. They wereclassified into ages such as 30-39, 40-49, 50-59 and 60-69, and 202 female and 212 male herders were chosen accidentally. The model types of research “Cross-Sectional” method is used for this, the equipment called “Tanita” which is used for measuring body is used for collecting information and also SPSS-22programmis used for producing result. RESULT OF STUDY: 1. 49% of all research participants are male, 51% are female. 2. Herder’s biological aging is 50,4+9,4 and it shows that 6,3 older than calendar ages. 3. Herders’ aging was different for male and female herders. 81,8 percent of the male herders get older very fast whereas 69,4 of female herders get older slowly. CONCLUSION: • Male herder’s aging is 14 years older than womenThe older female and male herders get, the less their difference in their biological age becomes. • The percentage of the herders whose aging is getting fast is 21-26 % older than biological aging of the UB city’s people. • Biological age and sex’s connection (r=0,0001 p=0,00488) between biological age and weight’s index is (r=0,00488, p=0,001) is different and statistical significant.
ABSTRACT
BackgroundIn the present time Photodynamic therapy is a largely experimental treatment modality which is under development for application in both of neoplastic and non–neoplastic diseases. PDT involves a light-sensitive compound (photosensitizer), light and molecular oxygen. The photosensitizer is excited to its singlet state by light of the appropriate wavelength. Responses to photodynamic treatment are dependent on the photosensitizer used the an illumination conditions, the oxygenation status of the tissue and the type of cells involved.CoalTo study new photosensitizers from the pyrazole derivatives which is based on chlorine and porphyrin and produced from sea algae to the lung cancer cell (A549) that is cultured in vitro medium and tested by two ways that are light treatment and dark treatment. Furthermore we define how the photosensitizer dictate cancer cells by test MTT/3-/4.5-dimethylthiazole-2- yl/-2.5-biphenyl tetrzolium bromide a yellow tetrazole/, and how to change the cell morphological characteristics by its micro photo and determine potosensitizers that dictates on the least doze of the cell.Materials and MethodsThe cell line tested was A549 (human lung carcinoma cell). The cell line was obtained from the cell line bank at Seoul National University’s Cancer Research Center (Korea) and were grown in medium RPMI-1640 (Sigma-Aldrich) with 10% fetal bovine serum, glutamine, penicillin and streptomycin at 37 0C in humidified atmosphere of 5% CO2 in air. Phosphate buffered saline (PBS) (Sigma-Aldrich), microscope (Olympus, CK40-32 PH, Japan), Laser irradiation (BioSpec LED 670-700 nm, Russia), ELISA-reader (BioTek, Synergy HT, USA), trypsin-EDTA solution, incubator (37 0C, 5% CO2) were used. The PDT was carried out using a diode laser generator apparatus (BioSpec LED, Russia) equipped with a halogen lamp, a band-pass filter (670-700 nm), and a fiber optics bundle. The wavelength was set at 670±1 nm. Duration of the light irradiation, under PDT treatment, is calculated taking into account the empirically found effective dose of light energy in J/cm2. Figure 1 shows micrographs from an optical microscope illustrating the morphological changes of A549 cells at different points of time after PDT.ResultsThe morphological changes for all tested photosensitizers were revealed by the same patterns, hence we have shown the morphological changes for compound 8 in this paper (Fig 1). Untreated A549 cells as a control did not show any significant morphological changes. The changes in the state and activity of cellular organelles induced by PDT were clearly observed after 3 h. PDT treatment against A549 cells induced plasma membrane disruption and cell shrinkage, indicating the plasma membrane as the main target for the photosensitizer: the membrane of A549 cells began to shrink immediately after PDT, and cell death processes commenced with cytoplasm leakage around the membrane for the first 3 h. After 24 h, the membrane had disintegrated, confirming the loss of cell viability.СоnclusionThe morphological changes were determined in 3 h, 24 h and 48 h after PDT. The bioactivity of 23 new photosensitizers was examined. Among them, 8 photosensitizers showed a promising effect for PDT, therefore, their in vitro biological results are displayed in this study. It was observed from the experimental results that the tested photosensitizers showed more promising effects for PDT or light toxicity. These photosensitizers inhibited more than 50% cells at 2.5 μM after 24 h. Untreated cells as control did not show any significant morphological changes. The PDT treatment cells induced to plasma membrane disruption and cell shrinkage. After 24 h of treatment the membrane was disintegrated, and confirmed the loss of cell viability.