ABSTRACT
The prevalence of hepatitis C virus (HCV) genotypes in Southern Brazil was studied in the plasma of 100 HCV-RNA-positive patients attended in Porto Alegre, South of Brazil. Reverse transcriptionpolymerase chain reaction (RT-PCR) products from the 5' noncoding region were double digested with RsaI-HaeIII and BstNI-HinfI and analyzed by restriction fragment length polymorphism (RFLP). Three genotypes (1, 2 and 3) were demonstrable, the most prevalent being HCV type 1 (55 of 100 patients, 55 per cent), followed by HCV type 3 (37 of 100 patients, 37 per cent) and HCV type 2 (8 of 100 patients, 8 per cent). There was an unusual high prevalence of genotype 3, in contrast to the majority of published data from the Southeast region.
Subject(s)
Humans , Hepacivirus/genetics , Brazil/epidemiology , Polymerase Chain ReactionABSTRACT
Já dispomos de informaçöes científicas que indicam a viabilidade técnica da produçäo de uma vacina contra carrapatos e outros artrópodes. Buscando alternativa de controle do Boophilus microplus, os autores estudaram essa questäo fundamental e urgente, devido à resistência às drogas e os malefícios de resíduos químicos nos produtos de origem animal e no meio ambiente