ABSTRACT
Background: Application of bio-stimulants is one of solutions to quantitative and qualitative changes in metabolites of medicinal plants sprouts
Objective: The aim of this study is to investigate the effect of chitosan bio-stimulation on trigonelline content of sprout under water stress
Methods: This study was conducted in three separate factorial experiments [three times of 4, 8 and 12 days after planting] based on completely randomized design with 15 treatment in 3 replications. The treatments were chitosan in five levels [0, 0.05%, 0.1%, 0.2% and 0.4%] and water stress in 3 levels [control without stress treatment, -0.2 and 0.4 Mpa]
Results: The result of variance analysis showed that chitosan, under stress and interaction effect of chitosan and water stress had significant effect on trigonelline content of 4, 8 and 12-day sprout. The trigonelline content decreased during the experiment in a way that the highest content of that was observed at the first days of seed germination
Conclusion: By induction of water stress and application of chitosan with optimum amount [to 0.2%] decrease in trigonelline content attained to the lowest trend during the experiment