ABSTRACT
Lead is one of the world wide using metals which have been used since ancient time. It is also a toxin, it has adverse effects on the body even at low level of exposure and it induces a broad range of physiological, biochemical, and behavioral dysfunctions. Studies have been showed that this metal has harmful effects on several tissues such as: nervous system, blood tissues, and cardiovascular system, reproductive and urinary system. Survey the effect of Lead acetate on hepatic tissues in light and Electron microscope. White male rabbits of New Zealand race were used and divided into two groups. Experimental groups [N =10] 6.5 mg/Kg of lead acetate were injected intra peritoneal and control group [N=10] were injected only with demonized water every day to each animal for 7 weeks. After taking biopsy from hepatic tissues of each group, tissue preparation was performed for LM and EM studies as standard method. Quantitave and qualitative [morphometeric and morphologic] studies were carried out on electro micrographs. Data was compared using statistically methods. Morphological and morphometerical findings showed the vacuolated of mitochondria of hepatocyte, degeneration of rough endoplasmic reticulum, heterochromatin of nuclei and reduce in lobular diameter in experimental group as compare with control group [P
Subject(s)
Male , Animals, Laboratory , Liver/drug effects , Liver/ultrastructure , Rabbits , Microscopy, Electron , Microscopy, PolarizationABSTRACT
Lead is one the world wide using metals it has been used since ancient time. It is also a toxin, known to have adverse effects on the body even at low level of exposure and it induces a bread range of physiological, biochemical, and behavioral dysfunctions. Studies have been showed that this metal has harmful effects on several tissues such as: nervous system, blood tissues, and cardiovascular system, reproductive and urinary system. Because it damage human, animal and plants. Nowadays has been attended on this metal. White male rabbits of New Zealand race were used and divided into two groups. Experimental groups [N=10] 6.5 Mg/Kg of lead acetate were injected intraperitoneally every other day to each animal for 7 weeks as chronic dose and control group [N=10] were injected only with demonized water. After taking biopsy from testis tissues of each group, tissue preparation was performed for LM and EM studies as standard method. Morphologic study was carried out on electron micrographs. Data have been compared using statistically methods. Morphological findings showed that testis tissue in experimental group that chronic dose has been sever changed histologically compared with control group. Seminifar tubules diameter showed significant decrease [p<0.05]. Primary Spermatocyte nucleus showed heterochromatin and mitochondria showed vacuelaution. These results [based on present study findings] revealed that lead acetate could have vivid effects on testis tissue during chronic dose
Subject(s)
Male , Animals , Lead/toxicity , Cytotoxins , Testis/drug effects , Testis/ultrastructure , RabbitsABSTRACT
Introduction: Ciprofloxacin is a synthetic antibacterial agent belonging to the family of Fluoroquinolones with a very broad spectrum against of microbial pathogens, especially Gram-negative infectious diseases, that has been approved in more than 100 countries world-wide
Objective: The aim of this study was planed to see determine the histopathological effects of ciprofloxacin after inducement, in rat testis
Materials and Methods: The twenty male wistar rats were selected and randomly divided into two groups control [n=10] and test [n=10]. The test group was received 12.5mg/kg [PO] ciprofloxacin daily for sixty day however the control group just received plate. In sixtieth day the testis tissue of rats in both groups were removed and prepared for electron microscopy
Results: Electron microscopic studies of testis tissue slices of test group showed many changes such as: nuclei of spermatocyt I and sertoli cells and myoid were hyperchromatined. The Mitochondria of spermatogonia and spermatocyts cells in test group were vacuolated when as compared with control group. Thickness of spermatid tail in test group was increased when as compared with control group
Conclusion: There was a marked decrease in fertility index, testicular weight in experimental group as compared with control group. Since in our study ciprofloxacin had side effects on testis tissue in rat. It was suggested that using ciprofloxacin can decreasing fertility rate in human