[Comparison of physical feed restriction methods during rearing on broiler breeder performance [growth and metabolic parameters]]

To compare the effects of four physical feed restriction methods during rearing broiler breeder on growth, metabolic status and blood indices of stress. A 4x2 factorial experiment based on a completely randomized design. On hundred and sixty pullets and 160 roosters of broiler breeder. The pullets and roosters were housed in pens and consumed the same amount of feed in both treatments. The effects of feed restriction methods [every day, six days a week, five days a week and skip-a-day] during 3 to 22 weeks of age on body weight, uniformity [coefficient variation], feed efficiency, serum biochemical parameters [glucose, triglyceride, total protein, cholesterol and uric acid] blood indices of stress [such as heterophil to lymphocyte ratio and basophil, monocyte and eosinophil counts] were determint. Analysis of variance [ANOVA]. While in the skip-a-day method the body weight and the feed efficiency were lower, the plasma cholesterol [P<0/05] and heterophil: lymphocyte ratio were higher in females. Because of higher body weight uniformity in five days a week and six days a week methods, these methods can be recommended

[Serological study on Sarcosystosis in slaughtered buffaloes [Bubalus buba] using IFAT compare with meat inspection finding in Ahvaz abattoir]

Objective: evaluation of IFAT and abattoir methods for identifying and study of buffalo sarcocystosis[Sarcocystis fusijormis]

Samples: a total of 398 serum samples were taken from buffaloes before slaughtering for IFAT studing the rate of sarcocystis infections and the results compared with meat inspection and laboratory finding [macro and micro cyst]

Procedure: before slaughtering, blood samples were taken from jagular vein for serological examination by IFA method. After slaughtering, esophagus, diaphragm, heart and skeletal muscles were examined for macroscopic cyst of sarcocystis .For microscopic cysts, the samples were taken from each one of these tissues for impression smear [Dob smear]. The macro cysts were identified as S.jiusijormis. Bradizoites of this sarcocyst were used as antigen in IFAT and rabbit antibuffalo conjugated serum for this test was prepared in central laboratory of faculty of veterinary medicine, University of Tehran [Dr.Reza Rastegar central laboratory] using standard method

Results: the results showed that macroscopic and microscopic infection rates of animals is 18.6% and 53.5% respectively. In this study, maximum rate of infection include macroscopic and microscopic finding was in eosophagus and minimum in heart muscle. Any significant dfferences were observed in infection rates due to sex. The infection rate in adult group was significantly more than young buEaloes. A significant correlation was observed between antibody titer and the rate of macroscopic and microscopic infection [P<0.05], increasing the antibody titer till 1:640 had positive correlation and more than this titre viceversa. All of slaughtered animals had atleast 1:40 titre and most of them were in 1:640 titer group [25.9%] and the lowest prevalence was in 1:10240 titer [1.5%]

Conclusion: according to the results, the IFAT is a suitable test for studing sarcocystosis in buffaloes and is useful for further studies about b s economically important parasite in Khoozestan province