ABSTRACT
Background: Lemon balm [Melissa officinalis] is one of the most valuable species of Labiateae family. In order to optimize producing secondary metabolisms in balm, the study was conducted to examine producing of dried material and medicinal substances by using methanol and MgSO4 spraying
Objective: According to provide maximum photosynthesis potential through new carbon and Mg sources and reduce loss of metabolic energies, it is probable to have maximum active substance by using the best recommendation of methanol and MgSO4 spraying on Melissa officinalis
Methods: A factorial experiment using the randomized complete design was conducted in the Science and Research Unit of the Islamic Azad University in 2013 with 12 treatments and 3 replications. The factors included 4 levels of methanol [0, 10, 20, and 40%] and 3 levels of manganese sulfate [0, 0.5, and 1 g/l]. After seedling transplantation and establishment, methanol was sprayed three times at 15-intervals at midday [one of these sprays was carried out together with the first methanol spray]. At the start of flowering, plant organs were harvested and foliage extraction was performed. The essential oil in each treatment was analyzed using GC and GC/MS
Results: The table of ANOVA showed the mutual effects of methanol and manganese sulfate on percentage essential oil and on its performance were significant at the 1% probability level
Conclusion: Results of GC and GC/MS indicated the maximum percentages of lemon balm essential oil constituents were those of the treatment in which 40% methanol and 0.5 g/l manganese sulfate were applied
ABSTRACT
Researchers' attention has been focused on Aloe vera for a long time due to the numerous medicinal effects of this plant and its application in various fields of food, cosmetic and hygienic industries. This study was conducted to evaluate the different levels of Zeolite and irrigation conditions on vegetative growth and quantitative and qualitative changes in plant gel. It is based on a factorial randomized complete block design with 4 levels of Zeolite in soil and 3 levels of irrigation, in 3 replications. After 5 months, morphological traits were measured. Also the amount of aloin in leaf and vitamin C in the gel were measured. The best results of morphological traits were obtained with 22 g Zeolite treatment in 5 kg pots of soil and 21 days irrigation. The highest amount of aloin was observed in 44 g Zeolite treatment in 5 kg pots of soil with 7 days irrigation. The highest amount of vitamin C was obtained in 44 g Zeolite treatment in 5 kg pots of soil with 14 days irrigation. The overall results showed that zeolite plays an important role in promoting growth and increasing secondary metabolites in plant Aloe vera under different irrigation