ABSTRACT
Introduction: Survivin, an inhibitor of apoptosis [IAP] containing one baculoviral IAP repeat [BIR] domain, has been reported to be able to regulate both cellular proliferation and apoptotic cell death. In the present study, we evaluated the differential expression of different variants of survivin during prenatal and postnatal development of brain in mice
Material and Methods A total of 27 NMRI mice were categorized into 9 age groups and brain specimens were obtained accordingly [n=3 per each group]: 11 and 17 days embryos and newborn from which the remaining brains were collected by intervals of 5 days up to 1 month of age. Total RNA was extracted from each brain and Reverse Transcription was performed by oligo dT and M-MLV enzyme. cDNAs were amplified with primers specific for survivin and beta2 microglobulin [as an internal control] via polymerase chain reaction technique
Results: We demonstrated that survivin is expressed during both fetal and postnatal development of brain in mice. RT-PCR performed on survivin showed two different variants of survivin with different intensities. The expression of the bigger variant [survivin140] during both prenatal development and at birth was significantly higher than its postnatal one
Conclusion: Our data suggests that the expression of survivin140 in brain is developmentally regulated; such a regulation may play a role in homeostasis of brain, and in refinement of synapses
ABSTRACT
Recently, a novel gene was cloned and characterized namedCatSper, which codes for a unique Ca2+ channel that is expressedexcludively in the testis. It has crucial role in sperm motility, spermpenetration into the ovum and ultimately male fertility. In the present study we have evaluated the expression of this gene at different ages of mouseby Semi-quantitative RT-PCR. Material and Total RNA was extracted from testis of mouse usingRNX plus solution. Reverse Transcription was done by oligo dT and MMLVenzyme. cDNAs were amplified in PCR reaction for both CatSper and b2m[as an internal control] genes. After gel electrophoresis, intensity of signalfor each band was quantified by Labimage software. 1] There was no d expression of CatSper gene in mouse testis of1 day, 1 week and 2 weeks [pre-mature group] of age;2] Expression ofCatSper began with the age of three weeks with a gradual increase till twomonths of age and a gradual slight decrease in older age groups;however, the observed pattern of expression appeared not to bestatistically significant [p=0.592]. These results confirm previous data; that is there is a linkbetween expression of CatSper and fertilization in mouse. The resultsfurther reveal a link between CatSper expression profile and the age ofmouse in terms of sexual maturity