ABSTRACT
Cardiac remodeling is defined as changes in shape and function of the heart in response to aggression (pressure overload). The sarcoplasmic reticulum calcium ATPase cardiac isoform 2a (SERCA2a) is a known factor that influences function. A wide spectrum of studies report a decrease in SERCA2a in heart failure, but none evaluate it's the role in early isolated diastolic dysfunction in supravalvular aortic stenosis (AoS). Our hypothesis was that SERCA2a participates in such dysfunction. Thirty-day-old male Wistar rats (60-80 g) were divided into AoS and Sham groups, which were submitted to surgery with or without aorta clipping, respectively. After 6 weeks, the animals were submitted to echocardiogram and functional analysis by isolated papillary muscle (IPM) in basal condition, hypoxia, and SERCA2a blockage with cyclopiazonic acid at calcium concentrations of 0.5, 1.5, and 2.5 mM. Western-blot analyses were used for SERCA2a and phospholamban detection. Data analysis was carried out with Student's t-test and ANOVA. AoS enhanced left atrium and E and A wave ratio, with preserved ejection fraction. Basal condition in IPM showed similar increases in developed tension (DT) and resting tension (RT) in AoS, and hypoxia was similar between groups. After cyclopiazonic acid blockage, final DT was equally decreased and RT was similar between groups, but the speed of relaxation was decreased in the AoS group. Western-blot was uniform in all evaluations. The hypothesis was confirmed, since functional parameters regarding SERCA2a were changed in the AoS group.
Subject(s)
Animals , Male , Aortic Stenosis, Supravalvular/complications , Hypertrophy, Left Ventricular/physiopathology , Sarcoplasmic Reticulum Calcium-Transporting ATPases/physiology , Ventricular Dysfunction, Left/physiopathology , Aortic Stenosis, Supravalvular/metabolism , Calcium-Binding Proteins/analysis , Collagen/analysis , Diastole/physiology , Disease Models, Animal , Echocardiography , Heart Ventricles/pathology , Heart Ventricles/physiopathology , Hypertrophy, Left Ventricular/etiology , Hypertrophy, Left Ventricular/metabolism , Hypoxia/metabolism , Hypoxia/physiopathology , Indoles , Myocardial Contraction/physiology , Rats, Wistar , Sarcoplasmic Reticulum Calcium-Transporting ATPases/analysis , Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism , Time Factors , Ventricular Dysfunction, Left/etiology , Ventricular Dysfunction, Left/metabolism , Ventricular Remodeling/physiologyABSTRACT
Provas funcionais respiratorias foram realizadas em 58 pacientes com Paracoccidioidomicose (Pbmicose) pulmonar uni e multifocal em 52; tumoral em 5 e genital feminino interno em 01. A idade oscilou de 20 a 74 anos e a duracao da doenca variou de 3 a 25 anos. Manifestacoes respiratorias, tegumentares e linfaticas foram predominantes. Catorze deles desenvolveram Cor pulmonale, funcao renal alterada em 19, modificacoes eletrocardiograficas em 8 e atividade hiporeatora adrenal em 13 dos 20 casos estudados. A analise radiologica revelou lesoes designadas: leve em 16; moderada em 24 e grave em 18 que a evolucao evidenciaram: manutencao e piora, respectivamente, em 35 e em 23 deles. As provas funcionais respiratorias mostraram: espirografia normal em 17; obstrutivo em 32 e misto em 9 doentes. O espaco morto foi superior a 35 por cento em 25 e a ventilacao alveolar minuto estava elevada em 54. A diferenca alveolo-arterial de Oxigenio estava aumentada em todos. A analise estatistica revelou associacao significativa entre radiologia: evolucao radiologica e a funcao pulmonar. A Pbmicose em doentes tabagistas inveterados conduziu ao enfisema, enquanto que as modificacoes alveolo-arteriais anoxemicas propiciaram a disseminacao da Doenca de Lutz.