ABSTRACT
This study was designed to determine which stage of Gnathostoma spinigerum and which method of the preparation of test antigens are the most suitable for the detection of antibodies in serum of rabbits infected with advanced third stage larvae (AL3) of G. spinigerum by the indirect fluorescent antibody test (IFAT). Antigens from parasite ova and first stage larvae (L1) were obtained from freshly preserved specimens and affixed to glass slides with egg albumin. AL3 antigens consisted of paraffin sections, cryostat sections and pellets of crude worm soluble extract. Slides of adult male and female worms were prepared in cryostat sections. Pellets of crude worm soluble extract (AL3) smeared onto slides gave the best positive reaction followed by AL3 cryostat sections and L1.
Subject(s)
Animals , Antibodies, Helminth/immunology , Antigens, Helminth/diagnosis , Evaluation Studies as Topic , Female , Fluorescent Antibody Technique/standards , Gnathostoma/immunology , Larva/growth & development , Male , Nematode Infections/blood , RabbitsABSTRACT
Pyrantel pamoate at dosage of 10 mg per kilogramme body weight was given to each villager at 4-6 weeks intervals for three times. The infection rates of hookworms among those who received and did not received the drug decreased from 60.5% pre-treatment to 19.6% after the third treatment and then increased to 32.4% one year after the third treatment. Infection rates of A. lumbricoides and Strongyloides spp. among the villagers who received and did not received the drug decreased from 27.5% pre-treatment to 2.2% after the third treatment and then increased to 20.1% one year later for the former, while the latter decreased from 6.5% pre-treatment to 2.3% after the third treatment and 0.4% one year later. The infection rate of T. trichiura was found to increase as pyrantel pamoate had no effect on this nematode. The authors suggested that to obtain more satisfactory results the anthelmintic drug used must likewise be effective against T. trichiura particularly in areas where this nematode is a public health problem. It was also suggested that the duration of treatment should be prolonged.