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1.
Chinese Journal of Epidemiology ; (12): 157-159, 2007.
Article in Chinese | WPRIM | ID: wpr-232330

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the prevalence of Anaplasma phagocytophilum in rodents from forest areas in northeastern China.</p><p><b>METHODS</b>PCR amplification, followed by sequence analysis was carried out. The sequences of 16S rRNA and gltA gene fragment amplified from rodent specimens were compared with corresponding part of the sequences deposited in GenBank.</p><p><b>RESULTS</b>A total number of 276 rodents were tested, including 102 in Jilin province, 61 in Helongjiang province and 113 in Inner Mongolia autonomous region. The positive rates were 8.82%, 1.64% and 0.00%, respectively. The infection rate in rodents infected by ticks was 11.30 times higher than that in rodents without ticks (P = 0.002). The S. A. phagocytophilum 16S rRNA sequences from rodents in Jilin and Heilongjiang were identical and differed in 3-5 bases compared with the corresponding parts of A. phagocytophilum from America, Sweden and Japan. Compared with the sequences registered in GenBank, the nucleotide sequence of gltA varied from 87%-97% and its deduced amino acid sequence changed from 84%-99%.</p><p><b>CONCLUSION</b>A. phagocytophilum infection was presented in rodents from Jilin and Heilongjiang province.</p>


Subject(s)
Animals , Amino Acid Sequence , Anaplasma phagocytophilum , Genetics , Bacterial Proteins , Base Sequence , China , Ehrlichiosis , RNA, Ribosomal, 16S , Rodentia , Microbiology , Ticks , Trees
2.
Chinese Journal of Epidemiology ; (12): 343-345, 2007.
Article in Chinese | WPRIM | ID: wpr-232307

ABSTRACT

<p><b>OBJECTIVE</b>To study the variation of specific antibody among convalescent of severe acute respiratory syndrome (SARS) patients through a three-year program.</p><p><b>METHODS</b>Sera samples were collected from SARS cases in the 5th, 20th and 35th month after onset of the illness. The SARS-CoV specific antibody was detected for all of them by ELISA and neutralized test simultaneously. The titer of neutralizing antibodies was calculated using Reed-Muench method, and the comparison between different time groups was analyzed regarding the variance of data on repeated measures after logarithm conversion.</p><p><b>RESULTS</b>13, 17 and 13 sera samples were collected in the 5th, 20th and 35th month after onset. Results showed that despite the fact that the positive rates of ELISA antibody were 100%, 82.4% and 84.6% respectively,the neutralizing antibody was still positive for all the samples. The average neutralizing antibody titers were 1:43 (1:16-1:203), 1:36 (1:17-1:59) and 1:21 (1:10-1:39) on the 5th, 20th and 35th month after onset, and the differences were statistically significant (F = 60.419, P < 0.001). On the 35th month after the onset, 30.8% (4/13) of the patients were still having the neutralizing antibody level of above 1:36, but the neutralizing antibody level in another 30.8% (4/13) of the patients had decreased to as low as 1:10, when the cut-off level was set as 1:8.</p><p><b>CONCLUSION</b>Results of the study indicated that the neutralizing antibody of SARS cases could last for at least three years, but the sera specific antibody in SARS cases decreased gradually when time went by. However, neutralizing antibody in some of the cases decreased to a lower level on the 35th month. Further follow-up study was worthwhile to observe the long-lasting profile of antibody existence on SARS cases.</p>


Subject(s)
Humans , Antibodies, Neutralizing , Enzyme-Linked Immunosorbent Assay , Follow-Up Studies , Severe Acute Respiratory Syndrome , Allergy and Immunology
3.
Chinese Journal of Epidemiology ; (12): 886-890, 2007.
Article in Chinese | WPRIM | ID: wpr-322903

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the genetic differences of Orientia tsutsugamushi (Ot) Sta56 gene between Shandong isolates and other strains deposited in GenBank.</p><p><b>METHODS</b>PCR and restriction fragment length polymorphism (RFLP) were used to amplify the complete sequence of Ot-Sta56 gene. RFLP profiles of Ot were predicted by a computer program according to their complete sequences of Ot-Sta56 gene. PCR amplicon from XDM2 strain was sequenced and analyzed by Clustal X (1.8) and PHYLIP software.</p><p><b>RESULTS</b>The complete sequences (about 1.6 kbp) of Ot-Sta56 gene were amplified from B16 strain (isolated from patients), FXS2 strain (isolated from A. agrarius) and XDM2 strain. Four species of restriction endonucleases (Hha I, Hinf I, Hae III, Pst I) were used to digest the PCR amplicons from the 3 isolates. When comparing with the RFLP profiles of prototype Ot, the RFLP profiles of PCR amplicons from the 3 isolates were similar to those of Japan Kawasaki strain, but were quite different from the international reference strains Gilliam, Karp, Kato. Results from DNA sequence analysis showed that the complete sequence of Ot-Sta56 gene homology to Japan Kawasaki strain of XDM2 strain was 97%, and deduced amino acid sequence was 92%.</p><p><b>CONCLUSION</b>Data from the complete sequence of Sta56 gene indicated that the genotypes of Ot isolates in Shandong province were similar, but with distinction from the Kawasaki strain.</p>


Subject(s)
Amplified Fragment Length Polymorphism Analysis , Antigens, Bacterial , Genetics , Bacterial Proteins , Genetics , Bacterial Typing Techniques , Classification , DNA, Bacterial , Genetics , Genes, Bacterial , Membrane Proteins , Genetics , Orientia tsutsugamushi , Genetics , Polymorphism, Restriction Fragment Length , Sequence Analysis, DNA
4.
Chinese Journal of Epidemiology ; (12): 482-486, 2007.
Article in Chinese | WPRIM | ID: wpr-294309

ABSTRACT

<p><b>OBJECTIVE</b>To confirm the existence of Amur-like viruses in Apodemus peninsulae in China, and to understand the molecular characteristics of these viruses.</p><p><b>METHODS</b>Total RNA was extracted from lungs of A. peninsulae captured in Jilin of Northeast China with Trizol reagent. Complete S and partial M segments of Amur virus were amplified and sequenced. Phylogenetic analyses on multiple nucleotide sequences were performed with the Clustal method and DNASTAR software.</p><p><b>RESULTS</b>383 bp cDNA of M segment and 1696 bp of S segment of Amur like virus were recovered from lung tissue of A. peninsulae, named JilinAP06. The full-length of its S gene comprised of 1696 nucleotides with ORF including 1287 nucleotides and encoding a protein which comprised 429 amino acids. The phylogenetic analysis of this sample with other hantaviruses revealed that the complete S and partial M segment sequence of JilinAP06 both were closely related to those Amur viruses such as AP63, AP61, AP1371 and AP1168 found in A. peninsulae from Far East region of Russia and B78 strain, Liu strain and H5 strain, which were all from Chinese patients. The complete S and partial M segment sequence of JilinAP06 had only 81.0% identities with the nucleotide sequences of HV prototype 76-118 strain.</p><p><b>CONCLUSION</b>Amur-like viruses did exist in A. peninsulae from Northeasern China while A. peninsulae might be the natural reservoir of Amur-like viruses in China and was the important infectious source to HFRS patients which were caused by Amur-like viruses.</p>


Subject(s)
Animals , Humans , China , Orthohantavirus , Classification , Genetics , Hantavirus Pulmonary Syndrome , Virology , Lung , Virology , Murinae , Virology , Open Reading Frames , Genetics , Phylogeny , Reverse Transcriptase Polymerase Chain Reaction
5.
Chinese Journal of Epidemiology ; (12): 475-478, 2006.
Article in Chinese | WPRIM | ID: wpr-233922

ABSTRACT

<p><b>OBJECTIVE</b>To investigate rodents' natural infection of Orientia tsutsugamushi (Ot) in some areas of Inner Mongolia and Xinjiang, China.</p><p><b>METHODS</b>DNAs were extracted from spleens of the captured mice and nested-polymerase chain reaction (nPCR) technique was used to detect the Ot-Sta56 gene. Six positive samples were sequenced and analyzed by Clustal X (5.0) and DNA Club software.</p><p><b>RESULTS</b>A total of 90 rodents were captured in Inner Mongolia, and the overall prevalence of Ot was 6.67%. There was no significant difference in infection rates among the positive rodents species. 20 rodents were captured in Xinjiang, and the prevalence of Ot was 5.00%. The geographical difference in infection rates was not statistically significant between Inner Mongolia and Xinjiang. 9 rodents were captured in farmlands of Inner Mongolia and Xinjiang but there was no positive samples found. 101 rodents were captured in grasslands, and the prevalence of Ot was 6.93%. The Sta56 gene nucleotide sequence homology to Karp strain of N59 (from Microtus maximowiczii), N69 (from Cricetulus barabensis) and X33(from Cricetus cricetus) was 99%. The sequence homology to Taitung-2 strain and TW461 strain of N65 (from C. barabensis) was 94%, and the sequence homology to Taitung-2 strain and TW461 strain of N88(from Apodemus agrarius) was also 94%. The sequence homology to Oishi strain of N90 (from A. agrarius) was 96.00%.</p><p><b>CONCLUSION</b>Our findings indicated that infections of Ot did exist in rodents captured from Inner Mongolia and Xinjiang. The genotypes of Ot in Inner Mongolia and Xinjiang were quite complex, with some of them belonged to Karp type, and the others belonged to Taitung-2, TW461 and Oishi types which providing evidence for further investigation on the scrub typhus fuci in the two areas.</p>


Subject(s)
Animals , China , Geography , Orientia tsutsugamushi , Genetics , Polymerase Chain Reaction , Rodentia , Microbiology , Scrub Typhus
6.
Chinese Journal of Epidemiology ; (12): 681-684, 2006.
Article in Chinese | WPRIM | ID: wpr-233895

ABSTRACT

<p><b>OBJECTIVE</b>To detect and study the types of Borrelia burgdorferi sensu lato in ticks and rodents from Da Xing-An Mountains Forest areas of China.</p><p><b>METHODS</b>Nested PCR was performed to amplify 5S-23S rRNA intergenic spacer of B. burgdorferi. Positive products were analysed by restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP), specimens showing unique RFLP profile were sequenced and analysed.</p><p><b>RESULTS</b>1336 Ixodes persulcatus, 144 Dermacento silvarum, 144 Haemaphysalis concinna and 145 rodents of 9 species were collected from 16 sections of Da Xing-An Mountains Forest areas of China. Specific fragments were amplified from 293 I. persulcatus and 6 D. silvarum and 5 rodents of 4 species. B. burgdorferi was not detected in H. concinna. Among the positively tested I. persulcatus, 209 contained B. garinii genospecies and 45 contained B.afzelii genospecies based on RFLP. Moreover, B.garinii genospecies consisted of B. garinii 20047 and B. garinii NT29. 17 adult I. persulcatus were simultaneously infected with B. garinii 20047 and B. garinii NT29. Nine adult I. persulcatus were simultaneously infected with B. garinii 20047 and B. afzelii. Four adult I. persulcatus were simultaneously infected with B. garinii 20047 and B. garinii NT29 and B. afzelii. Two D. silvarum were infected with B. garinii 20047, 1 D. silvarum with B. garinii 20047, 2 D. silvarum with B. afzelii. 3 rodents were infected with B. garinii 20047 while 2 rodents were infected with B. garinii NT29. Mixed infection was not found in D. silvarum and rodents. In addition, nine I. persulcatus and one D. silvarum specimens showed unique RFLP pattern. Data from sequential analysis showed that they all belonged to B. garinii. PCR-SSCP profiles of 5S-23S rRNA intergenic spacer of B. burgdorferi in the positive specimens exceeded 36 types; B. garinii 20047 showed 16 types while B. garinii NT29 showing 11 types, B. afzelii showing 9 types. SSCP profiles of the specimens coinfected with multiple B. burgdorferi was relatively complex.</p><p><b>CONCLUSION</b>The infection of B. burgdorferi was found in the ticks and rodents in Da Xing-An Mountains Forests areas. The infection rate of I. persulcatus was high. B. garinii was predominant genospecies, and the population of B. burgdorferi was heterogeneous in the area. Mixed infections of different B. burgdorferi genospecies in ticks were found. I. persulcatus and Clethrionomys rufocanus were possibly served as major vector and major host for B. burgdorferi, respectively, suggesting that further study is needed to confirm the coinfection in humans and animals in this region.</p>


Subject(s)
Animals , Humans , Borrelia burgdorferi Group , Genetics , China , Epidemiology , Lyme Disease , Epidemiology , Polymorphism, Restriction Fragment Length , Polymorphism, Single-Stranded Conformational , RNA, Bacterial , Rodentia , Microbiology , Ticks , Microbiology , Trees
7.
Chinese Journal of Epidemiology ; (12): 196-199, 2006.
Article in Chinese | WPRIM | ID: wpr-295579

ABSTRACT

<p><b>OBJECTIVE</b>To further understand the association of hantavirus (HV) harbored and transmitted in wild brown rats.</p><p><b>METHODS</b>Rattus norvegicus (n = 570) were trapped in 10 sites in Beijing. RT-PCR was used to test rodent lung samples for hantavirus infection. Unconditional multivariate logistic regression analysis was performed, with PCR positive as the dependent variable and the characteristics of Rattus norvegicus population as independent variables.</p><p><b>RESULTS</b>The overall HV prevalence in Rattus norvegicus was 9.1% (52/570). Significant association between HV infection in Rattus norvegicus and some biological characteristics of host population was observed. Adult Rattus norvegicus had a higher HV prevalence than juveniles. Males in the reproduction periods and rats with wounds were more likely to be infected with HV than others.</p><p><b>CONCLUSION</b>It was further confirmed that there existed parallel transmission of HV in Rattus norvegicus hosts. Aggression might be the primary mode of HV transmission among male Rattus norvegicus.</p>


Subject(s)
Animals , Female , Male , Rats , Aggression , Animals, Wild , Wounds and Injuries , Virology , China , Epidemiology , Orthohantavirus , Hantavirus Infections , Epidemiology , Virology , Logistic Models , Lung , Virology , Prevalence , Wounds and Injuries , Virology , Reproduction , Reverse Transcriptase Polymerase Chain Reaction , Risk Factors , Rodent Diseases , Epidemiology
8.
Chinese Journal of Epidemiology ; (12): 9-13, 2005.
Article in Chinese | WPRIM | ID: wpr-232145

ABSTRACT

<p><b>OBJECTIVE</b>To study the existence of Ehrluichiosis, lyme disease and tick-borne spotted fever coinfection in some areas in China.</p><p><b>METHODS</b>Using polymerase chain reaction (PCR), B. burgdorferi sensu lato, spotted fever group (SFG) Rickettsiae and human granulocytic ehrlichia (HGE), Ehrlichia chaffeensis (EC) were detected in ticks and mouse samples collected from Inner Mogolia autonomous region, Heilongjiang province, Beijing and Fujian province.</p><p><b>RESULTS</b>408 Ixodes persulcatus collected from Inner Mogolia autonomous region, HGE and B. burgdorferi sensu lato and SFG Rickettsiae were detected positive, with rates as 6.8%, 7.8%, 45.6%, respectively. 5 (5/408) were coinfection with HGE and B. burgdorferi sensu lato while 1 (1/408) was coinfection with HGE and SFG Rickettsiae. 46 Ixodes persulcatus collected from Helongjiang province were determined positive, with rates as 6.5%, 10.8% and 34.8%, respectively including 1 (1/46) coinfected with HGE and B. burgdorferi sensu lato. 2 of 922 ticks collected from Beijing were detected positive with B. burgdorferi sensu lato. Among 283 groups of Haemaphysalis yeni ticks (3/group) and from 38 rodent samples collected from Ninghua county of Fujian province HCE and B. burgdorferi sensu lato and SFG Rickettsiae were detected. Out of them, 25 groups were positive with EC and the minimal positive rate was 3.8% while 21 rodent samples were positive with EC with a positive rate of 56.4%. 2 ticks and 1 rodent sample were detected positive with EC and spotted fever group.</p><p><b>CONCLUSION</b>Coinfection of HGE and B. burgdorferi sensu lato or spotted fever group Richi did exist in Ixodes persulcatus collected from Inner Mogolia autonomous region and Heilongjiang province. Coinfection of EC and spotted fever group Richi was found in the ticks and rodents collected from Fujian province.</p>


Subject(s)
Animals , Humans , Rats , Arachnid Vectors , Borrelia burgdorferi Group , China , Epidemiology , DNA, Bacterial , Disease Vectors , Ehrlichia , Ehrlichiosis , Epidemiology , Ixodes , Microbiology , Lyme Disease , Epidemiology , Polymerase Chain Reaction , Rickettsia , Rickettsia Infections , Epidemiology , Rodentia , Microbiology , Tick-Borne Diseases , Epidemiology , Ticks , Microbiology
9.
Chinese Journal of Experimental and Clinical Virology ; (6): 340-343, 2005.
Article in Chinese | WPRIM | ID: wpr-333010

ABSTRACT

<p><b>BACKGROUND</b>To compare the biological characteristics of West Nile virus (WNV) and Japanese encephalitis virus (JEV), including cells sensitivity, pathogenicity, viral morphology, as well as the results of immunological and molecular biological detection.</p><p><b>METHODS</b>Cytopathic effect (CPE) and pathogenicity were observed in C6/36 cells and in suckling mice inoculated intracerebrally with the WNV or JEV, respectively. The sliced tissue samples for electron microscopic examination were prepared for the morphologic observation of the viruses. Serum antibody to WNV or JEV was detected using indirect immunofluorescence assay (IFA), and the viral RNA was analyzed by RT-PCR method.</p><p><b>RESULTS</b>WNV or JEV-caused CPE was characterized by cell fusion and cell shedding, respectively. There was no significant difference in the pathogenicity to suckling mice between WNV and JEV. The morphologic observation showed that the shape and size of the two virions were similar. WNV and JEV were found to have antigenic cross-reactivity. The viral RNA could be detected from both WNV and JEV samples with universal primer set, but only nucleoside fragments of corresponding virus could be amplified when specific primers were used.</p><p><b>CONCLUSION</b>CPE in C6/36 cell and detection of the viral RNA should be useful in discrimination of WNV and JEV, and simultaneously examining the titers of serum antibodies against WNV and JEV may be helpful to diagnosis of infection with these agents.</p>


Subject(s)
Animals , Mice , Brain , Virology , Cell Line , Diagnosis, Differential , Encephalitis Virus, Japanese , Allergy and Immunology , Encephalitis, Japanese , Diagnosis , Virology , Flavivirus Infections , Diagnosis , Virology , Immunoglobulin G , Blood , Mice, Inbred BALB C , West Nile virus , Allergy and Immunology
10.
Chinese Journal of Epidemiology ; (12): 574-577, 2005.
Article in Chinese | WPRIM | ID: wpr-331832

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the association between the genetic polymorphisms of myxovirus resistance 1 (MxA) gene and susceptibility to severe acute respiratory syndromes (SARS).</p><p><b>METHODS</b>A case-control study was conducted and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the T/G polymorphism at position-88 in the mxA gene promoter. Information on related factors of SARS was collected using a pre-testing questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package.</p><p><b>RESULTS</b>Sixty-six cases and sixty-four controls were selected for the study. Comparing with GG genotype, the proportion of GT genotype were significantly higher in the case group (81.3%) than that in the control group (62.5%)) with an OR (95% CI) of 2.700 (1.208-6.037). Multivariate logistic regression analysis revealed that the significant association remained after factors as wearing masks, protection gowns and eye-protection when contacting with SARS patient etc. were adjusted with an OR (95 % CI) of 2.911 (1.027-8.250).</p><p><b>CONCLUSION</b>mxA promoter-88G/T SNP might be confered to host genetic susceptibility to SARS in Chinese Han population.</p>


Subject(s)
Adult , Female , Humans , Male , Case-Control Studies , GTP-Binding Proteins , Genetics , Genetic Predisposition to Disease , Logistic Models , Multivariate Analysis , Myxovirus Resistance Proteins , Polymorphism, Genetic , Severe Acute Respiratory Syndrome , Genetics
11.
Chinese Journal of Epidemiology ; (12): 120-123, 2004.
Article in Chinese | WPRIM | ID: wpr-342373

ABSTRACT

<p><b>OBJECTIVES</b>To study the correlation between positive rates of RNA in clinical confirmed severe acute respiratory syndrome (SARS) patients and its appearance in relation to the development of the disease in order to provide scientific basis for early diagnosis, effective prevention and treatment of the disease.</p><p><b>METHODS</b>One-step reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the SARS RNA in the clinical specimens from different courses of the disease. The representative amplicons were then sequenced. Chi-square for trend test was performed to study the correlation between positive rates of RT-PCR and at different periods after the onset of the disease.</p><p><b>RESULTS</b>The fragments amplified from the sputum specimens of SARS patients were shown to share 100% homology with the published SARS-associated coronavirus. Of the different clinical specimens, positive rate in the stools appeared to be the highest (21.55%). Chi-square for trend test revealed that the positive rates of stools and sputa of SARS patients decreased with the development of the disease (chi(2) for trend = 12.55 and 16.408, P = 0.0004 and P = 0.000 05 respectively).</p><p><b>CONCLUSION</b>One-step RT-PCR proved to be an effective method for the detection of SARS-associated coronavirus from clinical specimens. Data as indicated that the positive rates of SARS coronavirus were decreasing in SARS patients along with the disease progression.</p>


Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Chi-Square Distribution , China , Disease Progression , Feces , Virology , Mucus , Virology , RNA, Viral , Genetics , Metabolism , Reverse Transcriptase Polymerase Chain Reaction , Severe acute respiratory syndrome-related coronavirus , Genetics , Severe Acute Respiratory Syndrome , Pathology , Virology
12.
Chinese Journal of Epidemiology ; (12): 421-424, 2004.
Article in Chinese | WPRIM | ID: wpr-342294

ABSTRACT

<p><b>OBJECTIVE</b>To investigate hantanvirus infection of captured rodents in Haidian district and Changping district of Beijing and to type hantavirus using molecular technique.</p><p><b>METHODS</b>The captured mice were classified and the density of distribution was calculated. Reverse transcription-polymerase chain reaction (RT-PCR) technique was used to amplify the partial M fragnments of hantaviruse. Several representative positive samples were sequenced and analysed by ClustalX (5.0) and DNAClub software.</p><p><b>RESULTS</b>A total of 414 animals were captured, among which Battus norvegicus was the dominant group. In Haidian district, the median infection rates with hantavirus were 13.14% in Battus norvegicus and 0 in Mus musculus Linnaeus. In Changping district, the average infection rates were 17.46% in Battus norvegicus and 3.57% in Mus musculus Linnaeus. Nucleotide sequences analysis showed that the virus detected all belonged to SEO-type. They clustered with Z37 virus and could be branched into 2 different subclades.</p><p><b>CONCLUSION</b>The major hosts of hantavirus in Haidian and Changping district were Battus norvegicus and the epidemic strains in the two districts of Beijing were genotyped as SEO-type. Nucleotide sequence and deduced amino acid sequence from different rodents were highly homologous, while nucleotide mutation had also been observed. Further studies are required to explore the possible virus sequence mutation.</p>


Subject(s)
Animals , Mice , Rats , China , Epidemiology , DNA, Viral , Genetics , Disease Reservoirs , Fluorescent Antibody Technique , Orthohantavirus , Classification , Genetics , Hantavirus Infections , Epidemiology , Virology , Hemorrhagic Fever with Renal Syndrome , Epidemiology , Virology , Molecular Epidemiology , Phylogeny , Reverse Transcriptase Polymerase Chain Reaction , Rodent Diseases , Epidemiology , Virology
13.
Chinese Journal of Epidemiology ; (12): 389-392, 2003.
Article in Chinese | WPRIM | ID: wpr-348860

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the association between the genetic polymorphisms of VDR gene and susceptibility to pulmonary tuberculosis.</p><p><b>METHODS</b>Case-control study was conducted. PCR-RFLP technique was used to detect the C/T polymorphism in VDR gene. Information on related factors of tuberculosis was collected using a pre-tested questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package.</p><p><b>RESULTS</b>A sample of 76 cases and 171 controls was studied. The genotype frequencies of VDR-FF, VDR-Ff and VDR-ff were 38.2%, 44.7%, 17.1% and 52.6%, 40.9%, 6.4% respectively. VDR-ff was significantly overrepresented in case group, the OR (95% CI) was 3.668 (1.483 - 9.071) when comparing with FF genotype. The significant association remained after adjusting BCG immunization and smoking, the OR (95% CI) was 3.036 (1.117 - 8.253).</p><p><b>CONCLUSION</b>The VDR-ff genotype might be associated with the susceptibility to pulmonary tuberculosis in Chinese Han population.</p>


Subject(s)
Adolescent , Adult , Child , Humans , Male , Middle Aged , Case-Control Studies , Genetic Predisposition to Disease , Genetics , Genotype , Logistic Models , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Receptors, Calcitriol , Genetics , Tuberculosis, Pulmonary , Genetics
14.
Chinese Journal of Epidemiology ; (12): 484-486, 2003.
Article in Chinese | WPRIM | ID: wpr-348829

ABSTRACT

<p><b>OBJECTIVE</b>To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.</p><p><b>METHODS</b>Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire.</p><p><b>RESULTS</b>The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%.</p><p><b>CONCLUSION</b>IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.</p>


Subject(s)
Humans , Antibodies, Viral , Blood , Enzyme-Linked Immunosorbent Assay , Fluorescent Antibody Technique, Indirect , Methods , Immunoglobulin G , Blood , Immunoglobulin M , Blood , Severe acute respiratory syndrome-related coronavirus , Allergy and Immunology , Severe Acute Respiratory Syndrome , Diagnosis
15.
Chinese Journal of Epidemiology ; (12): 1126-1128, 2003.
Article in Chinese | WPRIM | ID: wpr-246388

ABSTRACT

<p><b>OBJECTIVE</b>In order to find out the current situation of tick-borne spotted fever in the area of Changbai mountain, Jilin province.</p><p><b>METHODS</b>In this study, a polymerase chain reaction (PCR) method was developed with primers R. rOmpA 190.70p and R. rOmpA 190.701n designed on the basis of rOmpA gene, which is specific for examining spotted fever group Rickttsiaes (SFGR). Six hundred nighty-three ticks were tested and a positive PCR product amplified from D. silvarum specimen (named JL-02) was cloned and sequenced.</p><p><b>RESULTS</b>The SFGR DNA was detected from D. silvarum, Haemaphysalis concinna with the positive rates were 53.81% and 7.41% respectively. Its nucleotide sequence of 587 bp rOmpA and derived amino-acids showed 100.00% similarity with nucleotide sequence of DnS 14 and 99.00% with DnS 28 from the Former Soviet Union according to the result of BLUST and CLUSTAL, which was differential from the DNA sequences of strains previously detected in China.</p><p><b>CONCLUSION</b>The natural focus of tick-borne spotted fever did exist in the area of Changbai mountain. The DNA sequence of SFGR was similar to that of DnS 14, which was first reported in China.</p>


Subject(s)
Humans , Bacterial Outer Membrane Proteins , Genetics , China , DNA, Bacterial , Chemistry , Genetics , Phylogeny , Polymerase Chain Reaction , Rickettsia Infections , Microbiology , Rickettsieae , Classification , Genetics , Sequence Analysis, DNA
16.
Chinese Journal of Preventive Medicine ; (12): 408-411, 2003.
Article in Chinese | WPRIM | ID: wpr-291838

ABSTRACT

<p><b>OBJECTIVE</b>To investigate association between the natural-resistance-associated macrophage protein 1 (NRAMP1) gene polymorphisms and susceptibility to pulmonary tuberculosis (TB) in Chinese Han population.</p><p><b>METHODS</b>Hospital-based case-control study design was adopted. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) technique were used to type three NRAMP1 polymorphisms (INT4, D543N and 3'UTR). Information on related factors of tuberculosis was collected using a pre-tested standard questionnaire. Univariate and multivariate unconditional logistic analyses were conducted using SPSS for window software package. Totally, 110 cases of TB were selected during April 2001 to June 2002, with an average age of (27.7 +/- 12.7) years. Also, 180 cases of healthy control were selected, aged (27.3 +/- 9.2) years in average. Locus of NRAMP1 polymorphism was analysed with univariate method.</p><p><b>RESULTS</b>Univariate analysis demonstrated that the D543N G/A and 3'UTR TGTG+/del genotype occurred more frequently in the cases than in the controls, with crude odds ratios (OR) (95% CI) of 2.22 (1.03 - 4.78) and 1.93 (1.14 - 3.26), respectively. No significant association was observed between TB and INT4 polymorphisms. In multivariate analysis, associations of TB and D543N G/A and 3'UTR TGTG+/del genotypes remained, adjusted for exposure history and bacille Camette-Guérin immunization. Adjusted OR (95% CI) was 3.04 (1.12 - 8.27) and 2.36 (1.20 - 4.64), respectively. Still, no significant association between INT4 polymorphisms and TB was found.</p><p><b>CONCLUSION</b>Polymorphisms of D543N and 3'UTR locus in NRAMP1 gene might affect their susceptibility to TB in Chinese Han population.</p>


Subject(s)
Adolescent , Adult , Humans , Male , Case-Control Studies , Cation Transport Proteins , Genetics , Gene Frequency , Genetic Predisposition to Disease , Genetics , Genotype , Logistic Models , Polymerase Chain Reaction , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , Tuberculosis, Pulmonary , Genetics
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