ABSTRACT
Objetivos: identificar y determinar la circulación de los serotipos de virus dengue durante el brote producido entre los años 2000 y 2001 en la provincia de Coronel Portillo, departamento de Ucayali, Perú. Materiales y Métodos: Se trabajó con los casos probables de dengue que enviaron sus muestras al Instituto Nacional de Salud para su confirmación diagnóstica, se determinaron los casos de infección reciente a partir de ELISA de captura de IgM (MACELISA), se realizó el aislamiento en cultivos de células y la identificación por inmunofluorescencia indirecta (IFI). Resultados: Se procesaron un total de 742 muestras, se obtuvo que 19,1 por ciento resultaron positivos, 75,1 por ciento indeterminadosy 4,3 por ciento negativos. De las muestras positivas, 52,8 por ciento fueron mujeres y la población entre 11 a 40 años representó 66,9 por ciento de casos. Se realizaron 42 aislamientos en cultivo celular, identificando por IFI a 90,5 por ciento(38) comoserotipo 3 y al restante (4) como serotipo 1. Conclusiones: Durante el brote de dengue ocurrido entre los años 2000 y 2001 en la provincia de Coronel Portillo circularon dengue serotipo 1 y mayoritariamente el serotipo 3.
Objectives: To identify and determine the circulation of dengue fever virus serotypes during the 2000-2001 outbreak in Coronel Portillo province, Ucayali department, Peru. Materials and Methods: Probable dengue fever cases who had their blood samples sent to the Peruvian National Institutes of Health for diagnosis confirmation were studied, and recent infection cases were determined using a capture IgM ELISA test (MAC-ELISA). Viral isolation was performed using cell cultures, and identification was achieved using indirect immunofluorescence (IIF). Results: 742 samples were assessed, 19,1 per cent were found to be positive, 75,1 per cent were undetermined, and 4,3 per cent were negative. Of the positive samples, 52,8 per cent were from women, and 66,9 per cent of all cases were from persons between 11 and 40 years old. 42 viral isolates were obtained in cell culture, and 90,5 per cent (38) of them were identified as serotype 3 whit IIF, and the four remaining were identified as serotype 1. Conclusions: During the dengue outbreak between 2000 and 2001 in Coronel Portillo province, dengue fever virus serotype 1 and mostly serotype 3 were circulating in the affected area.