Cardioprotective effects of quercetin on doxorubicin induced cardiotoxicity in male rats

Background: Doxorubicin [Dox] is an effective anthracycline anticancer drug, although it's clinical efficacy is restricted because of several acute and chronic side effects such as cardiotoxicity. The aim of this study was to investigate the cardioprotective effects of quercetin on doxorubicin induced toxicity in male rats

Materials and methods: This experimental study was done on 30 Wistar rats, which divided into five groups [6 rats in each group]. One control group was treated with saline [1 mL/kg], while the second control group [quercetin vehicle] received DMSO [1 mL/kg] for 14 days. Experimental groups were orally treated with quercetin every day at a dosage of 20 mg/kg, doxorubicin [25 mg/kg, i.p.] on 12th, 13th and 14th days of the experiment, as well as, with the combination of doxorubicin and Quercetin in stated doses. The treatment period lasted for 14 days. Body weight and histological preparations of heart samples of treated animals were examined on 15th day

Results: Body weight animals treated with doxorubicin significantly decreased compared to other groups [p<0.05]. Quercetin was able to prevent weight loss caused by doxorubicin. Histopathological findings revealed that pretreatment by quercetin had protective efficacy against doxorubicin induced cardiotoxicity

Conclusion: According to pathological results, we confirmed that quercetin possess hepatoprotective effect against doxorubicin induced cardiotoxicity which may be through its antioxidant activity

Preparation of a selective l-phenylalanine imprinted polymer implicated in patients with phenylketonuria

Molecular imprinting is a method for synthesizing polymers with structure-selective adsorption properties with applications such as, selectivity binding, drug delivery systems and anti-bodies. The present study aims at optimizing the preparation of molecularly imprinted polymer [MIP] against 1-phenylalanine, in order to increase phenylalanine-binding in Enzymatic Intestinal Simulated Fluid [ESIF]. The MIP for 1-phenylalanine, as a water-soluble template, was successfully synthesized without derivatization. Synthesization was done by a UV polymerization method in which methacrylic acid [MAA], as a functional monomer, and ethylene glycol dimethacrylate [EGDMA], as a cross-linker, were used in the presence of five different porogenic solvents including; acetonitrile, tetrahydrofuran [THF], chloroform, toluene and dimethyl sulfoxide [DMSO]. The selectivity of the MIP was examined using 19 different amino acids in human serum and was evaluated by HPLC. In addition, morphological studies were conducted using SEM. The results showed that the obtained MIP with acetonitrile had the highest capacity and selectivity compared with other solvents. The data indicated that Phe-binding to MYS was significantly more than the former binding to NIP in EIS1 [p<0.05]. Moreover, in comparison wthNIP and control grouf MIP showed abetter selectivity and binding for Phe. This could be used for the reduction of Phe in human serum samples of Phenylketonuria. Our findings suggest that the MIP against PI prepared with acetonitrile, showed a good selectivity ai binding, which caused a reduction of blood Phe concentrati in enzymatic simulated intestinal fluid and human serum sam] of Phenylketonuria

effect of a hydro-alcoholic extract of olive fruit on reproductive argons in male sprague-dawley rat

Olive [Olea europea], from the Oleaceae family, is known as a phytoestrogen plant compound, containing Lignans and phenolic compounds. Some studies have shown phytoestrogens to have spermatogenesis-decreasing effects. The present study investigated the effects of a hydro-alcoholic extract of olive fruit on reproductive argons in male rats. The hydro-alcoholic olive [Olea europaea] extract was given orally to three experimental groups of rats in 50, 150, and 450 mg/kg in 48 days. The vehicle group was fed with normal saline and nothing was given to the control group [each group with 8 rats]. After 49 days reproductive indicators i.e., sperm count, sperm motility, the weight of prostate, testis, epididymis, and seminal vesicle were measured. The results showed a significant decrease in the weights of the left testicle, seminal vesicle, testosterone hormone, sperm count and sperm motility but there was no significant difference with regard to the weights of prostate and epididymis, and estradiol hormone. This study suggests that olive extract may have deleterious effects on fertility factors; therefore, after further studies, it may be used as a contraceptive in males

Chronic morphine consumption increase allograft rejection rate in rat through inflammatory reactions

Although opioids suppressive effects on immune system function have been reported, this study demonstrates inflammatory reactions, such as production of pro-inflammatory cytokines and suppression of anti-inflammatory cytokines, are the main causes at organ's allotransplantation rejection in chronic morphine-treated recipients. 28 rats were categorized in 4groups through intra-peritoneal administrations: control, sham, morphine treated animals [20 mg/kg injected of morphine daily until biopsy day], morphine and naloxane treated animals [20 mg/kg morphine and 2 mg/kg naloxane daily injected until biopsy day], which their donors were normal rats. The grafts were done at the 14[th] day of the experiment. Plasma interleukins levels [IL-6 and IL-10] in three sampling times were mwasured by ELISA, with almost 80% of macroscopic rejection signs in rats of one group, full thickness skin biopsy has been taken and histological parameters like perivascular infiltrates, epidermal changes, and stromal changes were detected. The statistical significance differences between the control and experimental groups were analyzed using the Kruskal-Wallis, followed by ANOVA post hoc test. Accelerated skin allograft rejection by chronic morphine consumption can be resulted of increased IL-6 concentration and decreased IL-10. The enhancing effects of morphine on the graft inflammation were partially antagonized by naloxane. It can illustrate the complexity of opiates and immune system connections and should be considered during organ transplantation of opiate addicts. Expression of skin cell in recipient with chronic morphine administration history maybe resulted in failure