Identification of hitherto undefined B-cell epitopes by antibodies in the sera of vitillgo patients using phage-display peptide library

A random 12 mers phage library was used to screen a pool of immunoglobulin fractions obtained from vitiligo patients. Subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. This panel was tested using an ELISA for their reactivity with pooled sera from patients and normal controls. Among the 16 randomly selected clones, two of clones showed distinct positive reactivity with the patient's sera compared with controls. The peptides displayed by these phages expressed the following amino acid sequences: SHMPLANQYQWA and NHVQAWEQFWDS. Thus, screening with phagedisplayed random peptide library of vitiligo sera can reveal peptide sequences that mimic vitiligo-related self-antigen

Clinical evaluation of Zataria multiflora essential oil mouthwash in the management of recurrent aphthous stomatitis

The purpose of this study was to evaluate the efficacy of the topical use of 0.2% oral mouth wash of essential oil of zataria multiflora in the control of recurrent aphthous stomatitis [RAS]. In this double-blind, randomized study each of 60 patients with RAS was assigned to one of the 2 management modalities. Those in group Areceived an oral rinse solution of Zataria multiflora essential oil, 3 times a day for 4 weeks and those in group B received placebo 3 times a day for 4 weeks. The patients were monitored two times weekly. A statistical Chi-Square test was performed to analyze the data. Zataria multiflora oral rinses was shown to be more efficacious than placebo in the treatment of patients with RAS