ABSTRACT
The methanol extract isolated from hermit crab, D. avarus degenerated ovarion and uterine tissues in cyclic and pregnant mice, treated before and after the implantation. Immunohistochemical staining using CD31 and Factor VIII specific to endothelial cells showed reduction in microvessel density. The hormonal assay showed decrease in the progesterone secretion in all experimental mice.
Subject(s)
Angiogenesis Inhibitors/pharmacology , Animals , Anomura , Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis , Embryo Implantation , Endothelial Cells/metabolism , Endothelium, Vascular/metabolism , Factor VIII/biosynthesis , Female , Fertility , Immunohistochemistry , Male , Methanol/metabolism , Mice , Microcirculation , Microscopy, Electron , Ovary/metabolism , Pregnancy , Pregnancy, Animal , Progesterone/blood , Time Factors , Uterus/metabolismABSTRACT
An organic extract from a marine crustacean D. avarus was examined for antiangiogenic activity by using the chick embryo chorioallantoic membrane (CAM) assay. The methanol extract (HCM) inhibited angiogenesis in a dose dependent manner. The extract was further fractionated by bioactivity-guided separation to purify the active fractions successively. This resulted in three fractions HCM1, HCM2 and HCM3. The 50% inhibition shown by HCM was 600 ng/disc, HCM1 was 100 ng/disc and of HCM3 was 2.7ng/disc. HCM3 which was separated by column chromatography and showed single spot on TLC was analysed by GLC and showed the presence of saturated and unsaturated fatty acids such as lauric, myristic, palmitic, stearic, oleic, linoleic. The antiangiogenic activity of the fatty acids obtained from a marine crustacean is reported for the first time.